Magana merip m6a kit

Manufactured by Merck Group

The Magana MeRIP m6A Kit is a laboratory equipment product designed for the detection and analysis of N6-methyladenosine (m6A) modifications in RNA samples. It provides a reliable and efficient method for the enrichment and identification of m6A-containing RNA fragments.

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Lab products found in correlation

Polya spin mrna isolation kit, by New England Biolabs (1 mentions) Anti m6a antibody, by Synaptic Systems (1 mentions) Direct zol rna miniprep kit, by Zymo Research (1 mentions) Rna clean concentrator 5, by Zymo Research (1 mentions)

2 protocols using magana merip m6a kit

Measuring m6A Modifications in mRNA

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mRNA was purified from total RNA by using a polyA Spin^TM mRNA Isolation Kit (NEB, Ipswich, MA USA). m6A modifications on target genes were detected using a Magana MeRIP m6A Kit (Millipore) according to the manufacturer’s instructions. In brief, 18 μg of purified mRNA was sheared into ~100 nt oligonucleotides in a fragmentation buffer and then incubated with anti-m6A antibody (Synaptic Systems)-conjugated or normal mouse IgG-conjugated beads at 4 °C overnight. Eluted RNA was then prepared for MeRIP-qPCR analysis.

Yang J., Qian X., Qiu Q., Xu L., Pan M., Li J., Ren J., Lu B., Qiu T., Chen E., Ying K., Zhang H., Lu Y, & Liu P. (2022). LCAT1 is an oncogenic LncRNA by stabilizing the IGF2BP2-CDC6 axis. Cell Death & Disease, 13(10), 877.

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Quantifying m6A in pre-miR-126 using meRIP-qPCR

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To determine m⁶A modification in pre-miR-126, meRIP-qPCR was performed using Magana meRIP m⁶A kit (17–10499, Millipore) according to the manufacture's instructions and previous reports.¹⁸ (link)^,¹⁹ (link)^,⁴⁵ (link)^,⁷⁸ (link) Briefly, total RNA was extracted using TRIzol and Direct-zol RNA miniprep kits (R2050, Zymo) following the manufacturer's instructions. The 300 ug total RNA was used for each IP-sample and one tenth of RNA was saved as input control. RNA was incubated with anti-m⁶A antibody or control mouse IgG antibody conjugated beads with rotation for 2 h at 4 °C. After washing 3 times with 1 × IP buffer, samples were eluted twice with m⁶A elution buffer. The eluted samples were purified using RNA Clean & Concentrator-5 (R1013 Zymo). Input RNA and MeRIP-ed RNA were further analyzed by qPCR. The enrichment of m⁶A in each sample was calculated by normalizing Ct values of the IP samples to the Ct values of the corresponding input portion.

Zhang Z., Zhou K., Han L., Small A., Xue J., Huang H., Weng H., Su R., Tan B., Shen C., Li W., Zhao Z., Qing Y., Qin X., Wang K., Leung K., Boldin M., Chen C.W., Ann D., Qian Z., Deng X., Chen J, & Chen Z. (2023). RNA m6A reader YTHDF2 facilitates precursor miR-126 maturation to promote acute myeloid leukemia progression. Genes & Diseases, 11(1), 382-396.

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