Trace 1310 gas chromatograph

Utilizing the cold percolation method, 200 g of the above sample of the plant was subjected to extraction. Subsequently, the extract was exposed to three separate applications of 500 mL of 70% methanol for 72 h at 25 °C. The methanol extract was filtered using a Buchner funnel. Then, the remaining methanol was entirely removed from the methanol extract using a rotary evaporator and concentration at low pressure at 40 °C. The sediment was dried in a desiccator to produce a dry weight yield of 20.68 g/100 g of T. purpurea subsp. apollinea, and GC-MS analysis was employed to identify the bioactive components [7 (link)].
A TRACE 1310 gas chromatograph connected to an ISQLT MS single quadrupole mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) was used. The data were obtained from the GC-MS at 70 eV ionization voltage, EI ionization mode, DB5-MS column with an internal diameter of 0.25 mm (J & W Scientific, Folsom, CA, USA), and the temperature was programmed in this manner: 3 min at 40 °C, 5 min at 280 °C, 1 min at 290 °C and constant at 7.5 °C/min. The detector and injector temperatures were adjusted to be 300 and 200 °C, respectively. The flow rate of the carrying gas (helium) was constant at 1 mL/min. The WILEY and NIST Mass Spectral Data Base was used as a search library [7 (link)].

The residual dichloromethane in DPH-loaded microparticles was measured using the headspace technique with a TRACE1310 gas chromatograph (GC; Thermo Fisher Scientific, Waltham, MA, USA) equipped with a TriPlus RSH autosampler and a TSQ9000 mass spectrometer (Thermo Fisher Scientific, USA). The sample was placed in a vial and heated before being injected into Rtx-624 column (30 m × 0.32 mm, 1.80 m; Restek Corporation, Bellefonte, PA, USA). The GC oven was initially set at 35 °C for 10 min, then increased to 200 °C for 2 min at a ramp rate of 10 °C/min.

The extracted oil was analyzed by a Thermos Scientific TRACE 1310 Gas Chromatograph (Waltham, MA, USA) attached to an ISQ LT single quadrupole mass spectrometer equipped with a capillary DB-1 column 15 m × 0.25 mm (J & W Scientific, Folsom, CA, USA). The injection port temperature was maintained at 200 °C, and the column oven temperature program was set from 115 °C (1 min) to 280 °C (3 min) (7.5 °C/min). The carrier gas was helium (1.5 mL/min), and the mass spectra were recorded at 70 eV. The chemical components were identified by comparing their mass fragmentation patterns with those of the standard reference data of the WILEY MASS SPECTRAL DATABASE.