Egf 236 eg 200

Manufactured by R&D Systems

Sourced in Switzerland, United States, Norway

EGF (236-EG-200) is a recombinant human epidermal growth factor (EGF) product. EGF is a small protein involved in the regulation of cell growth and differentiation.

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Lab products found in correlation

Epidermal growth factor (egf), by Merck Group (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) Hepatocyte growth factor (hgf), by Merck Group (1 mentions) Insulin transferrin sodium selenite supplement, by Roche (1 mentions) C11330500bt, by Thermo Fisher Scientific (1 mentions) Facsaria 2, by BD (1 mentions) Operetta cls high content imaging system, by PerkinElmer (1 mentions) Afatinib, by Selleck Chemicals (1 mentions) Plx4032, by Selleck Chemicals (1 mentions) Afatinib, by R&D Systems (1 mentions) Epidermal growth factor (egf), by Selleck Chemicals (1 mentions) Pcs 400 010, by American Type Culture Collection (1 mentions) P egfr y1068, by Abcam (1 mentions) Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions) Insulin, by Merck Group (1 mentions) Gentamycin, by Merck Group (1 mentions) P met y1234 1235, by Cell Signaling Technology (1 mentions) P 4e bp1 t37 46, by Cell Signaling Technology (1 mentions) Byl719, by Selleck Chemicals (1 mentions) Gdc 0941, by Selleck Chemicals (1 mentions)

Spelling variants of this product

EGF (236-EG) (13 citations)

6 protocols using egf 236 eg 200

Hepatic Differentiation of hADSCs and hUCMSCs

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hADSCs and hUCMSCs were seeded in 10-cm dish plates at a density of 5×10
⁴ cells/mL. Then, 100 ng/mL Activin A (CB031-0033; Shanghai ExCell Biology, Shanghai, China) was added to complete medium when the cells reached 50% confluence. The next day, cells were induced by the liver differentiation medium for 1 week. The hepatic differentiation medium contained 100 ng/mL HGF (10463-HNAS; Sino Biological), 20 ng/mL EGF (236-EG-200; R&D), 20 ng/mL IGF (291-G1-200; R&D), 20 ng/mL FGF-4 (CB055-0343; Shanghai ExCell Biology), 10 μg/mL Insulin-Transferrin-Sodium Selenite Supplement (11074547001; Roche, Basel, Switzerland), 1 μM dexamethasone (D4902-25MG; Sigma-Aldrich, St Louis, USA), 2 mg/mL fetal bovine serum (FBS 126575-10GM; Millipore, Billerica, USA), 1% penicillin-streptomycin (15070-063; Gibco) and DMEM-F12 medium (C11330500BT; Gibco). They were then transferred to a hepatic maturation DMEM-F12 medium that contained 40 ng/mL HGF, 20 ng/ml EGF, 20 ng/ml IGF, 20 ng/mL FGF-4, 10 μg/mL ITS, 1 μM dexamethasone, 1 mM nicotinamide (N0636-100G; Sigma-Aldrich), 20 ng/mL OSM (0452-HNAH; Sino Biological), 2 mg/mL FBS and 1% penicillin-streptomycin.

Jin Y., Shi R., Qi T., Li Q., Chen C., Gao S., Gao F., Yang D., Sun G., Xu J., Fu Q., Xu J, & Zhang X. (2023). Adipose-derived stem cells show hepatic differentiation potential and therapeutic effect in rats with acute liver failure: Adipose-derived stem cells for acute liver failure. Acta Biochimica et Biophysica Sinica, 55(4), 601-612.

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Culturing and Analyzing Pancreatic Cancer Cells

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Originally, PANC-1 and MIA-Paca-2 cells were cultured in DMEM supplemented with 10% FBS, then dissociated with trypsin solution. Dissociated single cells were transported to Falcon 5 mL polystyrene test tubes and washed twice with phosphate buffer solution (PBS). The cells were then analyzed on a flow cytometer (FACS Aria II, Becton Dickinson, San Diego, CA, USA). Then, PANC-1 and MIA-Paca-2 cells were separately sorted into ultra-low cluster 96-well plate with 200 μL sphere formation medium (SFM) added per well at the concentration of 100 cells/well. The SFM consisted of DMEM/F12 medium (11330-032, Gibco) supplied with 20 ng/mL epidermal growth factor (EGF, 236-EG-200, R&D Systems, Minneapolis, MN, USA), 20 ng/mL basic fibroblast growth factor (bFGF, 233-FB-025, R&D Systems), B27 supplement (17504044, Gibco) and N2 supplement (17502048, Gibco). Subsequently, cells were cultured at 37 °C in a 5% CO₂ humidified environment and imaged with an Operetta CLS high-content imaging system (PerkinElmer) every other day.

Shen Y., Pu K., Zheng K., Ma X., Qin J., Jiang L, & Li J. (2019). Differentially Expressed microRNAs in MIA PaCa-2 and PANC-1 Pancreas Ductal Adenocarcinoma Cell Lines are Involved in Cancer Stem Cell Regulation. International Journal of Molecular Sciences, 20(18), 4473.

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Targeted Inhibition of EGFR and MAPK Pathways in Thyroid Cancer Cells

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For the Afatinib assay, BCPAP and KTC-1 Cells (4×10⁵) were incubated with 100 ng/ml EGF (236-EG-200; R&D Systems) for 20 min, and then incubated with or without 1 μmol/L Afatinib (S1011; Selleckchem) for 2 h. For the PLX4032 assay, BCPAP and KTC-1 Cells (4×10⁵) were also incubated with 100 ng/ml EGF for 20 min and then incubated with or without 2 μmol/L PLX4032 (S1267; Selleckchem) for 4 h. After Afatinib and PLX4032 treatment, BCPAP and KTC-1 Cells were washed with ice-cold PBS three times, and whole-cell lysates were subjected to SDS–PAGE and incubated with p-EGFR^Y845, p-ERK1/2, t-ERK1/2, SLUG, ZEB1, ZEB2, and β-actin antibodies, respectively.

Zhan S., Wang T., Li J., Zhu H., Ge W, & Li J. (2022). Asporin Interacts With HER2 to Promote Thyroid Cancer Metastasis via the MAPK/EMT Signaling Pathway. Frontiers in Oncology, 12, 762180.

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Differentiating d-USC into RPTEC

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To evaluate the plasticity and differentiation capability of d-USC, we induced these cells to differentiate into human renal tubular epithelial cells (RPTEC). Both d-USC and USC (1,000 cells/cm², at p5) were induced to differentiate into RPTEC for 14 days, respectively. Renal tubule epithelial differentiation media consisted of a mixture of conditioned medium collected from cultured RPTEC and USC culture medium containing 30 ng/mL epidermal growth factor (EGF, 236-EG-200, R&D Systems) in a 1:1 ratio. The differentiation medium was replaced every third day. Cell morphology was observed before and after induction. Human RPTEC (ATCC® PCS-400-010™) were cultured as a positive control.

Xiong G., Tang W., Zhang D., He D., Wei G., Atala A., Liang X.J., Bleyer A.J., Bleyer M.E., Yu J., Aloi J.A., Ma J.X., Furdui C.M, & Zhang Y. (2019). Impaired Regeneration Potential in Urinary Stem Cells Diagnosed from the Patients with Diabetic Nephropathy. Theranostics, 9(14), 4221-4232.

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Investigating Receptor Tyrosine Kinase Signaling

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Reagents were: Insulin (Sigma, I0516), NEAA (Gibco, 11140050), gentamycin (Sigma, G1272), G418 (OZ Biosciences, GS21000).
Inhibitors were: BYL719 (Selleckchem, S2814), SHP099 (Selleckchem, S8278), GDC-0941 (Selleckchem, S1065), GS-493 (Millipore, 538099). All inhibitors were solved in DMSO.
Growth factors were: NRG1 (396-HB-050), EGF (236-EG-200) and FGF10 (345-FG-025), purchased from R&D Systems.
Antibodies: AKT (Cell Signaling, 9272), p-AKT S473 (Cell Signaling, 4060), p-4E-BP1 T37/46 (Cell Signaling, 2855), ERK (Cell Signaling, 9102), p-HER3 Y1289 (Cell Signaling, 4791), p-MET Y1234/1235 (Cell Signaling, 3077), p-FGFR Y653/654 (Cell Signaling 3471), p-S6 ribosomal protein S240/244 (Cell Signaling, 5364), p-ERK Y204 (Santa Cruz, sc-7383), HSP90 (Santa Cruz, sc-13119), SHP2 (Santa Cruz, sc-280), p-SHP2 Y542 antibody (AbCam, 62322), p-EGFR Y1068 (AbCam, 5644). Secondary rabbit (#111-035-144) and mouse (#115-035-062) antibodies were from Dianova.

Heynen G.J., Lisek K., Vogel R., Wulf-Goldenberg A., Alcaniz J., Montaudon E., Marangoni E, & Birchmeier W. (2022). Targeting SHP2 phosphatase in breast cancer overcomes RTK-mediated resistance to PI3K inhibitors. Breast Cancer Research : BCR, 24, 23.

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Propagation of Human Glioblastoma Cell Lines

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Human GBM cell lines (U251, T98, U87, and A172) were obtained from the Chinese Academy of Sciences Cell Bank (Shanghai, China). P3, the in vivo propagated primary GBM tumor cell line and the GFPluciferase stable U251 (GFP + U251) glioma cell line was kindly provided by Prof. Rolf Bjerkvig, University of Bergen. U251, T98, U87, and A172 Cells were grown in Dulbecco's modi ed Eagle's medium (DMEM; SH30022.01B, Gibco; Gaithersburg, MD, USA) supplemented with 10% fetal bovine serum (10082147 Hyclone; GE Healthcare Life Sciences; Pittsburgh, PA, USA), whereas P3 cells were grown in Neurobasal Medium (#21103-049, NBM; ThermoFisher Scienti c; Waltham, MA,) supplemented with 2% B27 (#A3653401, ThermoFisher Scienti c), 1% L-glutamine (#BE17-605E, BioNordika; Oslo, Norway), 1% penicillin/streptomycin (#17-603E, BioNordika) and 20 ng/mL EGF (#236-eg-200, R&D Systems; Minneapolis, MN) in 5% CO 2 in a humidi ed incubator at 37°C.

Hu Y., Xue Z., Qiu C., Feng Z., Qi Q., Wang J., Jin W., Zhong Z., Liu X., Li W., Zhang Q., Huang B., Chen A., Wang J., Yang N., & Zhou W. (2020). Knockdown of NUSAP1 Inhibits Cell Proliferation and Invasion Through Down-Regulation of TOP2A in Human Glioblastoma.

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