MKN28, AGS, MGC-803, SGC-7901, BGC-823, and MKN45 human gastric cancer cell lines and a normal gastric cell line GES-1 were obtained from Life Technologies (Gaithersburg, MD, USA) and were maintained in a humidified incubator at 37°C and 5% CO2. MGC-803, MKN45, and AGS cells were cultivated in RPMI-1640 medium (Life Technologies), and BGC-823, MKN28, and SGC-7901 cells were cultivated in DMEM medium (Life Technologies) plus 10 mM glucose, containing 10% fetal bovine serum, 100 μg/ml streptomycin, and 100 U/ml penicillin (all from Gibco, Grand Island, NY, USA).
Sgc 7901
Manufactured by Thermo Fisher Scientific
Sourced in United States, China
The SGC-7901 is a laboratory equipment designed for cell culture applications. It is a type of incubator that provides a controlled environment for the growth and maintenance of cells. The SGC-7901 is capable of maintaining precise temperature, humidity, and atmospheric conditions required for cell cultures.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 medium, by Thermo Fisher Scientific (84 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (69 mentions)
Bgc 823, by Thermo Fisher Scientific (64 mentions)
Mgc 803, by Thermo Fisher Scientific (49 mentions)
Ges 1, by Thermo Fisher Scientific (49 mentions)
Mkn45, by Thermo Fisher Scientific (35 mentions)
Hgc 27, by Thermo Fisher Scientific (33 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (31 mentions)
Rpmi 1640, by Thermo Fisher Scientific (31 mentions)
Penicillin, by Thermo Fisher Scientific (23 mentions)
Sgc 7901, by American Type Culture Collection (23 mentions)
Mkn28, by Thermo Fisher Scientific (22 mentions)
Streptomycin, by Thermo Fisher Scientific (20 mentions)
Sgc 7901, by National Collection of Authenticated Cell Cultures (19 mentions)
Bgc823, by National Collection of Authenticated Cell Cultures (13 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (11 mentions)
Mkn45, by American Type Culture Collection (11 mentions)
High glucose dmem, by Thermo Fisher Scientific (9 mentions)
Mgc 803, by National Collection of Authenticated Cell Cultures (9 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (9 mentions)
141 protocols using sgc 7901
1
Cultivation of Gastric Cancer Cell Lines
2
Modulating RASSF1A and miR-711 in Gastric Cancer
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The human gastric cancer cell line SGC-7901 was obtained from Shanghai Cell Bank, Chinese Academy of Sciences (Shanghai, China), and was cultured in RPMI 1640 medium (Life Technologies Inc., Grand Island, NY, USA) supplemented with 10% foetal bovine serum plus penicillin (50 IU/ml) and streptomycin (50 μg/ml) in a humidified incubator containing 5 ml/L CO2 at 37°C. The growth medium was refreshed every 2 days.
To manipulate RASSF1A expression in gastric cancer cells, we first cloned RASSF1A cDNA into a pCDA3.1 vector at the BamHI and XhoI sites. After DNA sequence confirmation, the pcDNA3.1-RASSF1A and control pcDNA3.1-vector were stably transfected into the SGC-7901 human gastric carcinoma cell line using Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA), according to the manufacturer's instructions, and SGC-7901 cells stably expressing the RASSF1A gene were established by G418 selection.
To determine the role of miR-711 in regulating the growth of SGC-7901 cells, we transfected SGC-7901 cells with the miR-711 overexpression vector (miR-711-mimics), miR-711 expression-inhibiting vector (miR-711-inhibitors) or empty vector (NC) (GenePharma Co., Ltd, Shanghai, China) and then assayed the viability of SGC-7901 cells by MTT 24 h, 48 h and 72 h after transfection. Transfection efficiency was tested at 24 h with GFP-labelled vector (Figure5 Bottom).
To manipulate RASSF1A expression in gastric cancer cells, we first cloned RASSF1A cDNA into a pCDA3.1 vector at the BamHI and XhoI sites. After DNA sequence confirmation, the pcDNA3.1-RASSF1A and control pcDNA3.1-vector were stably transfected into the SGC-7901 human gastric carcinoma cell line using Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA), according to the manufacturer's instructions, and SGC-7901 cells stably expressing the RASSF1A gene were established by G418 selection.
To determine the role of miR-711 in regulating the growth of SGC-7901 cells, we transfected SGC-7901 cells with the miR-711 overexpression vector (miR-711-mimics), miR-711 expression-inhibiting vector (miR-711-inhibitors) or empty vector (NC) (GenePharma Co., Ltd, Shanghai, China) and then assayed the viability of SGC-7901 cells by MTT 24 h, 48 h and 72 h after transfection. Transfection efficiency was tested at 24 h with GFP-labelled vector (Figure
3
Establishing Gastric Cancer Cell Lines
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GC cell line SGC7901 was ordered from Cell Bank of China Academy of Sciences (Shanghai, China). ADR-resistant SGC7901 cells (SGC7901/ADR) and VCR-resistant SGC7901 cells (SGC7901/VCR) were purchased from Shanghai MEIXUAN Biological Science and Technology Co., ltd (Shanghai, China). SGC7901, SGC7901/ADR and SGC7901/VCR cells were cultured in RPMI-1640 medium (Thermo Scientific, Rockford, IL, USA) supplemented with 10% fetal bovine serum (FBS, Thermo Scientific).
4
Establishing DDP-resistant Gastric Cancer Cells
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Human gastric cancer cell SGC7901 was provided by the Shanghai Cell Collection (Shanghai, China). Cells were cultured in RPMI-1640 medium (Gibco Laboratories, USA) containing 10% (v/v) fetal bovine serum, 1% penicillin–streptomycin 100 U/ml penicillin, and 100 μg/ml streptomycin in a humidified atmosphere of 5% CO2 in air at 37°C.
DDP-resistant SGC7901/DDP cells were induced from SGC7901 cells, using a concentration gradient method to increase the half maximal inhibitory concentration (IC50) of DDP (as previously described) [7 (link)].
DDP-resistant SGC7901/DDP cells were induced from SGC7901 cells, using a concentration gradient method to increase the half maximal inhibitory concentration (IC50) of DDP (as previously described) [7 (link)].
5
Gastric Cancer Cell Lines Cultivation
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The human gastric cancer cell lines, AGS, BGC-823, Hs746T, N87, KATOIII, SGC-7901 and SGC-7901/DDP (a cisplatin-resistant cell line), were obtained from the Molecular Pathology Laboratory at Mount Sinai Medical Center (New York, NY, USA). The BGC-823, SGC-7901 and SGC-7901/DDP cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS; Gibco, Grand Island, NY, USA). The AGS cells were grown in Ham's F12 medium. The Hs746T cells were cultured in DMEM containing 10% FBS. The KATOIII cells were maintained in IMDM mixed with 20% FBS. All media contained penicillin (100 U/ml) and streptomycin (100 U/ml), and all cells were cultured at 37°C in a humidified incubator at 5% CO2.
6
Gastric Cancer Cell Line Culture
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Human GC cell lines AGS, NCI-N87, BGC-823, HGC-27, KATO III, SGC-7901, SNU-1, SNU-5, and SNU-16 were purchased from American Type Culture Collection (ATCC). AGS, BGC-823, HGC-27, KATO III, and SNU-5 cells were cultured in DMEM-GlutaMAX medium (Life Technologies) supplemented with 10% fetal bovine serum (FBS; Life Technologies), penicillin (100 U/ml), and streptomycin (100 μg/ml; Life technologies). NCI-N87, SGC-7901, SNU-1, and SNU-16 cells were cultured in RPMI 1640-GlutaMAX medium (Life Technologies) supplemented with 10% FBS, penicillin (100 U/ml), and streptomycin (100 μg/ml). All the cells were maintained in a 5% CO2 humidified atmosphere at 37°C. The ATG5 and ATG7 siRNAs were purchased from Life Technologies. CQ, cobalt chloride (CoCl2) and Sp600125 were purchased from Sigma. Anisomycin was purchased from Cell Signaling Technology. Recombinant CXCL10 protein, CXCL10 antibody and mouse IgG1 isotype control were purchased from R&D systems. The plasmid pIREShyg3 was purchased from GenScript and the coding sequence (CDS) of CXCL10 gene was cloned in pIREShyg3 using Nhel / BamHI to obtain the pIREShyg3-CXCL10 plasmid.
7
Culturing Human Cell Lines
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Human gastric epithelial cell line, GES-1, was obtained from Cancer Institute and Hospital, Chinese Academy of Medical Sciences (Beijing, China). Human gastric cancer cell lines, AGS, MGC-803 and SGC-7901, were obtained from Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (Shanghai, China). Human embryonic kidney cell line, HEK 293T, was obtained from GeneChem Co., Ltd. (Shanghai, China). GES-1, AGS, MGC-803 and SGC-7901 were grown in RPMI Medium 1640 (Life Technologies) plus 10% fetal bovine serum (FBS). HEK 293T was grown in DMEM (Life Technologies) plus 10% FBS. All cell lines were grown at 37°C in a humidified atmosphere with 5% CO2. Cells were counted using a TC10 Automated Cell Counter (Bio-Rad).
8
Cell Line Cultivation and Reagent Use
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The BGC-823 and SGC-7901 cells were purchased from the Cell Bank of the Chinese Academy of Sciences, Shanghai, China, and the multi-drug-resistant signet ring cell GCSR1 [33 (link)] was a general gift of professor Li-song Teng. Cell morphology of GCSR1 in culture maintained in vitro for over 90 passages, is similar to the cells from the patient. The BGC-823 and GCSR1 cells were cultured in RPMI 1640 medium (Life Technologies, USA) supplemented with 10% FBS, and the SGC-7901 cells were supplemented with 20% FBS. African green monkey kidney Vero cells were purchased from the American Type Culture Collection (ATCC, USA), and cultured in DMEM (Life Technologies, USA) supplemented with 10% FBS. All cells were cultured at 37 °C with 5% CO2 and saturated moisture. Reagents: cisplatin (Sigma, P4394), Z-VAD-FMK (ApexBio, A1902), Methyl-β-cyclodextrin (Sigma, 332615), imipramine (ApexBio, C4117).
9
Gastric Epithelial Cell Culture Protocols
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Human normal gastric mucosa epithelial cell line GES-1 was obtained from Gefan Biological Technology (Shanghai, China). Human gastric cancer cell lines HGC-27, AGS, SGC-7901 were purchased from the Institutes for Biological Sciences at the Chinese Academy of Sciences (Shanghai, China). Human gastric cancer cell line MGC-803 was obtained from the Cell Resource Center, Institute of Basic Medicine, Chinese Academy of Medical Sciences (Beijing, China). GES-1, HGC-27 and SGC-7901 cells were cultured in RPMI 1640 medium (Life Technologies, Carlsbad, CA, USA) with 10% fetal bovine serum (FBS; Life Technologies) at 37 °C in humidified air with 5% CO2. The other cell lines were cultured in high-glucose DMEM (Life Technologies) supplemented with 10% FBS. Cells have been regularly tested for Mycoplasma and are free of contamination.
10
Gastric Cancer Cell Line Culture
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Human gastric cancer cell lines HGC-27 and SGC-7901 were purchased from Cell Bank,Type Culture Collection Committee,Chinese Academy of Sciences (Shanghai, China). HGC-27 cells and SGC-7901 cells were maintained in high-glucose DMEM (H-DMEM, Life technologies, USA) with 10 % FBS. HucMSCs were obtained and identified as previously described [27 (link)]. HucMSCs were maintained in low-glucose DMEM (L-DMEM, Life technologies) with 10 % FBS. Cells were all incubated at 37 °C in humidified cell culture incubator with 5 % CO2 and the medium was changed every 3 days after the initial plating.
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