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Slideviewer

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The SlideViewer is a high-performance digital slide scanner designed for use in pathology and research laboratories. It captures high-resolution digital images of microscope slides with a focus on speed, precision, and image quality.

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Lab products found in correlation

Dm500, by Leica (1 mentions) Alexa fluor 488 anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Alexa fluor 568 anti mouse igg, by Thermo Fisher Scientific (1 mentions) Zen lite software, by Zeiss (1 mentions) Anti cd3, by Agilent Technologies (1 mentions) Lsm 780 confocal microscope, by Zeiss (1 mentions) Mounting medium with dapi, by Abcam (1 mentions) Anti ki67 clone d2h10, by Cell Signaling Technology (1 mentions) Anti cd3 clone cd3 12, by Abcam (1 mentions) Mowiol, by Merck Group (1 mentions) Pannoramic p1000, by 3DHISTECH (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Wuhan Servicebio Technology (1 mentions) Masson s trichrome, by StatLab (1 mentions)

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SlideViewer software (10 citations) SlideViewer 2.6 (10 citations) SlideViewer Image software (2 citations)

9 protocols using slideviewer

1

Cardiomyocyte Morphometric Analysis

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To perform morphometric analyses, we measured the width and length of cardiomyocytes in µm. The latter parameter was obtained by measuring the distance between two intercalated discs. The analyses were performed with the hematoxylin-eosin (H-E) heart sections using a Slide Viewer application (SlideViewer, 3D Histech, Hungary). Measurements were performed under 40× objective magnification. The length of cardiomyocytes was measured, as demonstrated in Figure 1.
Wilk A., Król M., Kiełbowski K., Bakinowska E., Szumilas K., Surówka A, & Kędzierska-Kapuza K. (2024). Immunolocalization of Matrix Metalloproteinases 2 and 9 and Their Inhibitors in the Hearts of Rats Treated with Immunosuppressive Drugs—An Artificial Intelligence-Based Digital Analysis. Biomedicines, 12(4), 769.
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2

Histological Analysis of Fish Liver Tissue

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Six fish were selected at random from each category, and liver tissue samples measuring 0.5 cm × 0.5 cm × 0.5 cm were rinsed with 0.6% saline and then immersed in a 4% paraformaldehyde solution for 48 h. The liver tissue samples were then fixed, dehydrated, embedded, and stained with hematoxylin and eosin (HE), periodic acid Schiff (PAS), or oil red O (ORO) before being examined under light microscopy (DM500, Leica, Leica Microsystems (Schweiz) AG, Heerbrugg, Switzerland). Liver micrographs were captured at 20 × and 40 × magnification and recorded using a digital camera following the procedures outlined in our laboratory’s previous publication [39 (link)]. K-Viewer (https://kv.kintoneapp.com/en/user/, accessed 31 March 2023) 1.0 software (1.0.4) (Konfoong Bioinf Tech Co., Ltd, Ningbo, China) and Slide Viewer (accessed 21 April 2023) 2.5 software (2.5.0) (3DHISTECH Ltd., Budapest, Hungary) were utilized for image analysis conducted by Wu et al. [39 (link)]. Each analysis was conducted with 3 duplicates.
Xie Y., Shao X., Zhang P., Zhang H., Yu J., Yao X., Fu Y., Wei J, & Wu C. (2024). High Starch Induces Hematological Variations, Metabolic Changes, Oxidative Stress, Inflammatory Responses, and Histopathological Lesions in Largemouth Bass (Micropterus salmoides). Metabolites, 14(4), 236.
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3

Multiparametric Immunofluorescence Staining of Synovial Tissue

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Formalin Fixed Paraffin sections were made from synovial tissue explants from right and left knees of an ICI-arthritis patient. The staining shown in Fig. 1 and Fig. S1 was performed at the Molecular Cytology Core Facility at Memorial Sloan Kettering Cancer Research Center. Antibodies used included anti-CD8A (clone SP57, Ventana), anti-CD3 (polyclonal, Dako), anti-Ki67 (clone D2H10, Cell Signaling Technology) and anti-PD-1 (clone NAT105, Ventana). mIF slides were analyzed using SlideViewer (3DHISTECH v2.5) and the final images were rendered using ImageJ (v2.1.0). The staining shown in Fig. S8 included antibodies anti-CD8 (clone SP16, Invitrogen), anti-CD3 (clone CD3–12, Abcam) and anti-CD38 (clone 38C03 (SPC32), Invitrogen). Secondary antibodies were raised in goat and included anti-rabbit IgG AlexaFluor 488 (Invitrogen) for detection of CD8, anti-rat IgG AlexaFluor 647 (Invitrogen) for detection of CD3 and anti-mouse IgG AlexaFluor 568 (Invitrogen) for detection of CD38. Mounting Medium with DAPI (Abcam) was used for nuclei identification and mounting. The slides for Fig. S8 were imaged using a Zeiss LSM 780 confocal microscope and analyzed using Zen Lite software (Zeiss).
Wang R., Singaraju A., Marks K.E., Shakib L., Dunlap G., Adejoorin I., Greisen S.R., Chen L., Tirpack A.K., Aude C., Fein M.R., Todd D.J., MacFarlane L., Goodman S.M., DiCarlo E.F., Massarotti E.M., Sparks J.A., Jonsson A.H., Brenner M.B., Postow M.A., Chan K.K., Bass A.R., Donlin L.T, & Rao D.A. (2023). Clonally expanded CD38hi cytotoxic CD8 T cells define the T cell infiltrate in checkpoint inhibitor-associated arthritis. Science immunology, 8(85), eadd1591.
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4

Immunofluorescence Imaging of Tissue Sections

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Frozen 8-µm-thick tissue sections (n = 4) were fixed in 4% paraformaldehyde solution for 10 min and washed with PBS (pH 7.4) for 10 min. Sections were permeabilized with 0.3% Triton X-100 in PBS for 5 min and blocked for 1 h with 0.03% Triton X-100 in PBS containing 1% bovine serum albumin. Sections were incubated overnight at 4 °C with primary antibodies followed by 10 min washing with PBS. The sections were incubated with the corresponding fluorescent secondary antibodies for 2 h at 37 °C, counterstained with 2-(4-amidinophenyl)-1H-indole-6-carboxamidine (DAPI, Vectashield, Vector Laboratories, Newark, USA) to visualize the nuclei, and coverslips were mounted with Mowiol® (Sigma-Aldrich). The slides were scanned with a digital slide scanner (Pannoramic P1000, 3DHistech Ltd., Budapest, Hungary), and the images were viewed with Slideviewer (3DHistech Ltd., Budapest, Hungary).
Dillemuth P., Karskela T., Ayo A., Ponkamo J., Kunnas J., Rajander J., Tynninen O., Roivainen A., Laakkonen P., Airaksinen A.J, & Li X.G. (2024). Radiosynthesis, structural identification and in vitro tissue binding study of [18F]FNA-S-ACooP, a novel radiopeptide for targeted PET imaging of fatty acid binding protein 3. EJNMMI Radiopharmacy and Chemistry, 9, 16.
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5

Immunohistochemical Analysis of 3xTg-AD Mice

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The brain samples were embedded in paraffin wax and sliced. For immunohistochemistry analysis, formalin-fixed paraffin-embedded brain sections (ultrathin sections of 60–80 nm) of 3×Tg-AD mice were incubated overnight at 4°C with 4′,6-diamidino-2-phenylindole (DAPI) (Cat# G1012, Servicebio, Wuhan, China). Primary antibodies including anti-Aβ1–42 (CT) rabbit antibody (Cat# bs-23379R, Bioss, Beijing, China), NeuN (D4G4O) XP rabbit mAb (Cat# 24307S, CST), LC3A/B antibody (Cat# 4108S, CST), SQSTM1/p62 (D6M5X) rabbit mAb (Cat# 23214S) were used for specific target detection respectively. The horseradish peroxidase-labeled secondary antibodies including goat anti-mouse (1:200) or goat anti-rabbit (1:200) were adopted. The images were captured by SlideViewer (3DHISTECH, Budapest, Hungary), and the intensity of staining was quantified by using ImageJ software.
Tang Y., Wei J., Wang X.F., Long T., Xiang X., Qu L., Wang X., Yu C., Xiao X., Hu X., Zeng J., Xu Q., Wu A., Wu J., Qin D., Zhou X, & Law B.Y. (2024). Activation of autophagy by Citri Reticulatae Semen extract ameliorates amyloid-beta-induced cell death and cognition deficits in Alzheimer’s disease. Neural Regeneration Research, 19(11), 2467-2479.
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6

Histological Analysis of Broiler Ileum

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The broiler ileal tissues were fixed with 4% paraformaldehyde, embedded in paraffin and cut into 5 μm sections. Tissues were stained with hematoxylin and eosin (HE) after deparaffinization (Changchun Xavier Biotechnology Co. Changchun, China). Under a light microscope (X 40 magnification) with the Slide Viewer (version 2.5.0; 3DHISTECH Ltd., Budapest, Hungary) image-analyzing system, villus height (μm) and crypt depth (μm) were measured, and V/C was calculated61 (link).
Dong L., Li Y., Zhang Y., Zhang Y., Ren J., Zheng J., Diao J., Ni H., Yin Y., Sun R., Liang F., Li P., Zhou C, & Yang Y. (2023). Effects of organic zinc on production performance, meat quality, apparent nutrient digestibility and gut microbiota of broilers fed low-protein diets. Scientific Reports, 13, 10803.
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7

Histological Evaluation of Mouse Lungs

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The mouse lungs were isolated and fixed using 4% paraformaldehyde overnight. Additionally, then, the lungs were embedded in paraffin and cut into 5 μm sections. For histological assessments, the sections were stained with hematoxylin and eosin (H&E) and Masson’s trichrome (American MasterTech, Lodi, CA, USA). Staining was prepared according to the manufacturer’s protocol. All stained slides were filmed by OCUS40 Microscope Scanner (Grundium, Tampere, Finland) under the same conditions and pictured using SlideViewer (3DHISTECH, Budapest, Hungary).
Park J., Jang J., Cha S.R., Baek H., Lee J., Hong S.H., Lee H.A., Lee T.J, & Yang S.R. (2022). L-carnosine Attenuates Bleomycin-Induced Oxidative Stress via NFκB Pathway in the Pathogenesis of Pulmonary Fibrosis. Antioxidants, 11(12), 2462.
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8

Liver Histopathological Assessment

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Then, 4% paraformaldehyde fixative was used to immerse part of the hepatic lobes cut from C57BL/6 J mice for 48 h. Then, paraffin was used to embed the samples. Next, 5-μm thick sections were subjected to haematoxylin and eosin (H&E) and immunohistochemical (IHC) staining of PDPK1. Livers embedded in optimum cutting temperature compound were used for oil red O staining for the assessment of hepatic steatosis. The sections were finally scanned under SlideViewer (3DHISTECH Ltd.).
Zhang Y., Ding Y., Zhao H., Wang Z., Zeng F., Qian Z., Li J., Ma T, & Huang C. (2022). Downregulating PDPK1 and taking phillyrin as PDPK1-targeting drug protect hepatocytes from alcoholic steatohepatitis by promoting autophagy. Cell Death & Disease, 13(11), 991.
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9

Histological Analysis of Goose Intestine

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Samples were embedded in paraffin wax, sectioned at 7 μm, and stained with haematoxylin and eosin (Changchun Xavier Biotechnology Co., Changchun, China). The villus height and crypt depth were measured in three replicates per goose under a light microscope (X 40 magnification) with Slide Viewer (version 2.5.0; 3DHISTECH Ltd., Budapest, Hungary)image-analyzing system and calculated the ratio of villus height to crypt depth. The measurement standards and methods were referred to Abolfathi et al. [20 (link)].
Zheng J., Liang S., Zhang Y., Sun X., Li Y., Diao J., Dong L., Ni H., Yin Y., Ren J., Yang Y, & Zhang Y. (2022). Effects of Compound Chinese Herbal Medicine Additive on Growth Performance and Gut Microbiota Diversity of Zi Goose. Animals : an Open Access Journal from MDPI, 12(21), 2942.
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