ASA (Yoshida Pharmaceutical Co., Ltd., Tokyo, Japan) or celecoxib (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan) was suspended in 5% gum arabic (Wako, Japan). After fasting for 10–16 h, rats were orally administrated by gavage ASA solution (75 mg/kg B.W.), or celecoxib solution (30 mg/kg B.W.), or vehicle solution (5% gum arabic, 5 mL/kg), and paw withdrawal threshold was evaluated 1 h after administration of the drug.
Celecoxib
Manufactured by Tokyo Chemical Industry
Sourced in Japan
Celecoxib is a chemical compound that is commonly used as a pharmaceutical ingredient. It functions as a selective cyclooxygenase-2 (COX-2) inhibitor. Celecoxib is often used in the development and production of various drug formulations.
Automatically generated - may contain errors
Lab products found in correlation
Gum arabic, by Fujifilm (1 mentions)
Ethanol, by Thermo Fisher Scientific (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Polyethylene glycol 400, by Merck Group (1 mentions)
Pooled plasma from male sprague dawley rats, by Innovative Research (1 mentions)
Nicardipine hydrochloride, by Tokyo Chemical Industry (1 mentions)
Cp690550, by Selleck Chemicals (1 mentions)
Ebastine, by Tokyo Chemical Industry (1 mentions)
Lansoprazole, by Tokyo Chemical Industry (1 mentions)
Sofalcone, by Fujifilm (1 mentions)
Mouse il 6, by R&D Systems (1 mentions)
Tramadol, by Merck Group (1 mentions)
Duloxetine, by Tokyo Chemical Industry (1 mentions)
Venlafaxine, by Merck Group (1 mentions)
Methyl cellulose 400 solution, by Fujifilm (1 mentions)
Flurbiprofen, by Merck Group (1 mentions)
Bay11 7082, by Fujifilm (1 mentions)
5 fluorouracil 5 fu, by Fujifilm (1 mentions)
Abt 263, by Selleck Chemicals (1 mentions)
Hepatocyte growth factor (hgf), by Thermo Fisher Scientific (1 mentions)
8 protocols using celecoxib
1
Oral Administration of ASA and Celecoxib in Rats
2
Simultaneous Quantification of Dutasteride and Ketoprofen
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Dutasteride (purity ≥ 99%) was purchased from Carbosynth Korea (Seoul, Korea). H-DUT (purity > 98%) was purchased from Toronto Research Chemicals Inc. (North York, ON, Canada). KET (purity > 98%) and celecoxib (purity > 98%; as an internal standard; Figure 1 ) were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Pooled plasma from male Sprague–Dawley rats was purchased from Innovative Research, Inc. (Novi, MI, USA). Nicotinamide adenine dinucleotide phosphate (NADPH), rat liver microsomes (RLM; from male Sprague–Dawley rats), and human liver microsomes (HLM) were purchased from BD-Genetech (Woburn, MA, USA). HPLC-grade acetonitrile (ACN), dimethyl sulfoxide, methanol, and ethanol were purchased from Thermo Fisher Scientific, Inc. (Waltham, MA, USA). Polyethylene glycol 400 was purchased from Sigma-Aldrich Co. (St. Louis, MO, USA).
3
Screening of Small Molecule Inhibitors
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CP-690550 was purchased from Selleck Chemicals (Houston, TX, USA). Human oncostatin M (OSM), mouse IL-6 and mouse sIL-6Ra were purchased from R&D systems (Minneapolis, MN, USA). Ebastine, cetirizine hydorochloride, meloxicam, loxoprofen sodium hydrate, nicardipine hydrochloride, amlodipine besylate, flavoxate hydrochloride, lansoprazole, indometacin and celecoxib were purchased from Tokyo Chemical Industry (Tokyo, Japan). Sofalcone and benidipine hydrochloride were purchased from Wako Pure Chemical Industries (Osaka, Japan).
4
Analgesic Drug Evaluation in SNL Rat Model
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Five drugs were tested in this study: duloxetine, celecoxib (Tokyo Chemical Industry Co., Ltd, Tokyo, Japan), pregabalin (ChemFaces, Hubei, China), venlafaxine, and tramadol (Sigma-Aldrich, St. Louis, MO, USA). All drugs were dissolved in distilled water (Otsuka Pharmaceutical Factory Inc, Tokushima, Japan) containing 0.5% w/v methylcellulose 400 solution (Fujifilm Wako Pure Chemical Corporation, Osaka, Japan) and administered orally (5 mL/kg) on Day 7 after SNL.
5
Oral Administration of NSAIDs
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CDDP was purchased from FUJIFILM Wako Pure Chemical Corp. (Osaka, Japan).
Celecoxib was purchased from the Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan).
Flurbiprofen was purchased from Sigma Aldrich (St. Louis, MO, USA). All other chemicals and reagents were commercially available and of the highest purity possible. NSAIDs were chosen for the following reasons: (1) having a dosage form for oral administration; (2) they were not a prodrug; (3) easily available.
Celecoxib was purchased from the Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan).
Flurbiprofen was purchased from Sigma Aldrich (St. Louis, MO, USA). All other chemicals and reagents were commercially available and of the highest purity possible. NSAIDs were chosen for the following reasons: (1) having a dosage form for oral administration; (2) they were not a prodrug; (3) easily available.
6
Investigating Combination Anti-Cancer Treatments
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5-Fluorouracil (5-FU) and BAY11-7082 were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Celecoxib was obtained from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). ABT-263 was obtained from Selleck Chemicals (Houston, TX).
7
Hepatocyte Differentiation from iPSCs
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Human iPSCs (on-feeder culture) were differentiated into hepatocytes based on our study [14 (link)]. Briefly, human iPSCs were treated with activin A for 5 days. Then, differentiated cells (1 × 105 cells/cm2) were subcultured onto Matrigel-coated 24- or 96-well plates. Human iPSCs cultured without feeder cells were differentiated into endodermal cells using the ACP protocol. Endodermal cells (2 × 105 cells/cm2) were passaged onto Matrigel-coated 24- or 96-well plates. The subcultured cells were first cultured in the presence of 1% DMSO for 7 days, and then Cosmedium 004 (Cosmo Bio Co.) supplemented with 10 ng/mL hepatocyte growth factor (PeproTech, Inc.), 20 ng/mL oncostatin M (Wako Pure Chemical Industries), 100 nM dexamethasone (Wako Pure Chemical Industries), and 2 mM valproic acid (Wako Pure Chemical Industries) for 7 days. During the last 3 days, 25 μM celecoxib (Tokyo Chemical Industry Co. Ltd., Tokyo, Japan) was added to the culture. The medium was then changed to Cosmedium 004 supplemented with 10 ng/mL hepatocyte growth factor, 20 ng/mL oncostatin M, 100 nM dexamethasone, and 25 μM celecoxib for 3 days. Finally, cells were cultured in Cosmedium 004 containing 25 μM celecoxib for 4 days.
The catalog number of reagents used for cell differentiation were summarized inTable S1 .
The catalog number of reagents used for cell differentiation were summarized in
8
Celecoxib and CCCP-Induced Apoptosis Signaling
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Celecoxib was purchased from Tokyo Chemical Industry (Tokyo, Japan). Carbonyl cyanide mchlorophenylhydrazone (CCCP) and anti-β-actin (2F3) antibody were purchased from FUJIFILM Wako Pure Chemical Corporation (Tokyo, Japan). Anti-TMEM117 antibody was purchased from Abgent (San Diego, CA, USA). Anti-C/EBP homologous protein (CHOP, L63F7), anti-cleaved caspase-3 (5A1E), and anticleaved caspase-8 (18C8) antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA).
Anti-phospho PERK (poly6494), anti-XBP-1s, and anti-BCL-2 antibodies were obtained from BioLegend (San Diego, CA, USA). Anti-Mouse IgG, HRP-Linked Whole Ab Sheep and anti-Rabbit IgG, and HRP-Linked Whole Ab Donkey antibodies were purchased from GE Healthcare (Tokyo, Japan). Z-VAD-FMK was purchased from Peptide Institute, Inc. (Osaka, Japan).
Anti-phospho PERK (poly6494), anti-XBP-1s, and anti-BCL-2 antibodies were obtained from BioLegend (San Diego, CA, USA). Anti-Mouse IgG, HRP-Linked Whole Ab Sheep and anti-Rabbit IgG, and HRP-Linked Whole Ab Donkey antibodies were purchased from GE Healthcare (Tokyo, Japan). Z-VAD-FMK was purchased from Peptide Institute, Inc. (Osaka, Japan).
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