Quemesa
The Quemesa is a high-performance laboratory equipment designed for scientific applications. It serves as a versatile instrument capable of performing various analytical tasks. The core function of the Quemesa is to provide reliable and accurate measurements, enabling researchers and scientists to conduct their experiments and analyses with precision.
Lab products found in correlation
35 protocols using quemesa
Ultrastructural Analysis of Melanocyte Organelles
TEM Imaging of Hydrophilic Gel Samples
Transmission Electron Microscopy Imaging
Liposomal Vesicles Characterization via TEM
Characterization of Ferrous Sulfate-Loaded Nanoliposomes
Fe-nanoliposomes were also observed by the optical microscope in fluorescence field Axioplan 2- Image Zeiss, Jena, Germany, equipped with software to capture the images. The Rhodamine B dye was used to visualize lipid vesicles with a 100 X oil immersion objective.
Transmission Electron Microscopy of Small Extracellular Vesicles
For transmission electron microscopy (TEM), sEV preparations were loaded on copper formvar/carbon coated grids (Ted Pella). Fixation was performed with 2% paraformaldehyde in 0.1 M phosphate buffer (pH 7.4), followed by a second fixation with PBS 1% glutaraldehyde in PBS. Samples were stained with 4% uranyl acetate in methylcellulose.
For immunolabeling electron microscopy (IEM), sEV preparations were loaded on grids and fixed with 2% paraformaldehyde in 0.1 M phosphate buffer (pH 7.4). Immunodetection was performed with a mouse anti-human CD63 primary antibody (Abcam ab23792). Secondary incubation was next performed with a rabbit anti mouse Fc fragment (Dako Agilent Z0412). Grids were incubated with Protein A-Gold 10 nm (Cell Microscopy Center, Department of Cell Biology, Utrecht University). A second fixation step with 1% glutaraldehyde in PBS was performed. Grids were stained with uranyl acetate in methylcellulose.
All samples were examined with a Tecnai Spirit electron microscope (FEI, Eindhoven, The Netherlands), and digital acquisitions were made with a numeric 4k CCD camera (Quemesa, Olympus, Münster, Germany). Images were analyzed with iTEM software (EMSIS) and statistical studies were done with Prism-GraphPad Prism software (v8).
Immunoelectron Microscopy of Fixed Cells
Immunodetection of Extracellular Vesicles
Phage Visualization by Electron Microscopy
Immunolabeling and TEM Imaging of HTT Aggregates
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