One color low input quick amp labeling kit

Manufactured by Agilent Technologies

Sourced in United States

The One-color Low Input Quick Amp Labeling Kit is a laboratory product designed for the amplification and labeling of low-input RNA samples. The kit provides a streamlined workflow for preparing samples for microarray analysis.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Low Input Quick Amp Labeling Kit (367 citations) Quick Amp Labeling Kit (196 citations) One-Color Quick Amp Labeling kit (28 citations) Low Input Quick Amp kit (9 citations) Low Input Quick Amp Labeling (7 citations) One-Color Microarray-Based Gene Expression Analysis (Low Input Quick Amp Labeling) kit (3 citations) One-Color Low Input Quick Amp kit (2 citations)

54 protocols using one color low input quick amp labeling kit

MSCs-TCs Interaction Gene Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

To define alterations of gene expression profiles during MSCs‐TCs interaction, gene expression profiles were evaluated in co‐culture of MSCs and TCs at the concentration of 10⁵, as compared with MSCs or TCs alone. We also investigated potential effects of activated TCs or MSCs stimulated with LPS on MSCs or TCs, as compared with MSCs or TCs alone, or co‐culture of TCs and MSCs without activation. Gene expression profiles were measured as previously described.⁷ Briefly, total RNAs were isolated, amplified, labeled by One‐Color Low Input Quick Amp Labeling Kit (Agilent technologies, Santa Clara, CA, US), and purified using RNeasy mini kit (QIAGEN, GmBH, Germany). Each slide was hybridized using Gene Expression Hybridization Kit (Agilent) in Hybridization Oven (Agilent), washed in dishes with Gene Expression Wash Buffer Kit (Agilent) for 16 h, and then scanned using Agilent Microarray Scanner equipped with default settings. Data were measured with Feature Extraction software 10.7 and raw data were normalized with Quantile algorithm, Gene Spring Software 11.0 (Agilent).

Zhang D., Song D., Shi L., Sun X., Zheng Y., Zeng Y, & Wang X. (2020). Mechanisms of interactions between lung‐origin telocytes and mesenchymal stem cells to treat experimental acute lung injury. Clinical and Translational Medicine, 10(8), e231.

+ Open protocol

+ Expand

Marigold SFE Transcriptome Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

MiaPaca-2 cells were plated (2 × 10⁶) in p100 plates and 12 h later were treated for 48 h with 30 and 70 μg/ml of marigold SFE. Non-treated cells were kept as controls. Total RNA was isolated with the RNeasy Mini Kit (Qiagen Iberica). The microarray gene expression analysis between control and treated cells was performed by the Genomic Service of the National Center of Biotechnology (CNB-Madrid, Spain). After RNA integrity validation, RNAs were reverse transcribed and fluorescently tagged with the one-color Low Input Quick Amp Labeling Kit (Agilent Technologies). The microarray gene expression platform used was the Agilent Sure Print G3 Human 8 × 60 K (Whole Human Genome Microarray Kit).

Gómez de Cedrón M., Mouhid L., García-Carrascosa E., Fornari T., Reglero G, & Ramírez de Molina A. (2020). Marigold Supercritical Extract as Potential Co-adjuvant in Pancreatic Cancer: The Energetic Catastrophe Induced via BMP8B Ends Up With Autophagy-Induced Cell Death. Frontiers in Bioengineering and Biotechnology, 7, 455.

+ Open protocol

+ Expand

Genome-wide Microarray Analysis of Cell Differentiation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total RNA was extracted from frozen cell samples using ISOGEN (NIPPON GENE) according to the manufacturer’s instructions. Samples were analyzed using an Agilent SurePrint G3 Human GE 8 × 60K Microarray Kit (G4851A) and a one-color Low Input Quick Amp Labeling Kit (Agilent). Arrays were scanned using a G2505C Microarray Scanner System (Agilent). Raw microarray data were submitted to the Gene Expression Omnibus (GEO) microarray data archive (http://www.ncbi.nlm.nih.gov/geo/) at NCBI (accession number GSE55428).
Data were analyzed using Gene-Spring GX12.0 software (Agilent) after applying two normalization procedures. First, signal intensities of less than 1 were set to 1, and then each chip was normalized to the 75th percentile of all measurements from that chip. Baseline transformation of these data was not performed. Genes with a flag value of “detected” or “not detected” in at least one sample were analyzed. Differentially expressed genes were selected if the average values of each sample were altered by at least 2.0-fold. Early differentiation marker genes for the heat map were selected as previously described [21 (link)]. GO terms enriched with a p-value cut-off of 0.01 were extracted.

Onuma Y., Higuchi K., Aiki Y., Shu Y., Asada M., Asashima M., Suzuki M., Imamura T, & Ito Y. (2015). A Stable Chimeric Fibroblast Growth Factor (FGF) Can Successfully Replace Basic FGF in Human Pluripotent Stem Cell Culture. PLoS ONE, 10(4), e0118931.

+ Open protocol

+ Expand

Microarray Transcriptional Profiling of Mouse Colon

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total RNA was isolated using the protocol described above. RNA integrity was verified using a Bioanalyser 2100 with RNA 6000 Nano chips (Agilent Technologies, Palo Alto, CA, USA). Transcriptional profiling was performed on mouse colon samples using the SurePrint G3 Mouse GE 8 × 60 K Microarray kit (design ID: 028005, Agilent Technologies). Cyanine-3 (Cy3)-labeled cRNAs were prepared with 100 ng of total RNA using a One-Color Low Input Quick Amp Labeling kit (Agilent Technologies), following the recommended protocol. The specific activities and cRNA yields were determined by using a NanoDrop ND-1000 (Thermo Fisher Scientific, Waltham, MA, USA). For each sample, 600 ng of Cy3-labeled cRNA (specific activity > 11.0 pmol Cy3/μg of cRNA) were fragmented at 60 °C for 30 min and hybridized to the microarrays for 17 h at 65 °C in a rotating hybridization oven (Agilent Technologies). After hybridization, the microarrays were washed and then immediately dried. After washing, the slides were scanned using a G2565CA Scanner System (Agilent Technologies) at a resolution of 3 μm and a dynamic range of 20 bits. The resulting TIFF images were analyzed using the Feature Extraction Software v10.7.3.1 (Agilent Technologies) according to the GE1_107_Sep09 protocol. The microarray data were submitted to GEO under accession number GSE67577.

Lamas B., Richard M.L., Leducq V., Pham H.P., Michel M.L., Da Costa G., Bridonneau C., Jegou S., Hoffmann T.W., Natividad J.M., Brot L., Taleb S., Couturier-Maillard A., Nion-Larmurier I., Merabtene F., Seksik P., Bourrier A., Cosnes J., Ryffel B., Beaugerie L., Launay J.M., Langella P., Xavier R.J, & Sokol H. (2016). CARD9 impacts colitis by altering gut microbiota metabolism of tryptophan into aryl hydrocarbon receptor ligands. Nature medicine, 22(6), 598-605.

+ Open protocol

+ Expand

Gene Expression Profiling of Tumor Samples

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Purified total RNA for GEP was then amplified and fluorescently labeled using a One-Color Low Input Quick Amp Labeling Kit (Agilent Technologies). Cy3-labeled cRNAs were hybridized to a SurePrint G3 Human Gene Expression 8 × 60K v2 Microarray (Agilent Technologies). A signature analysis based on the gene expression was performed using the expression ratio of the tumor and the corresponding normal tissue (T/N). The expression signature/score was calculated from the average of genes in unique gene sets corresponding to each individual signature (TP53 inactivation^{28 (link)}, chromosomal instability [CIN]^{49 (link)}).

Imamura T., Ashida R., Ohshima K., Uesaka K., Sugiura T., Ohgi K., Yamada M., Otsuka S., Hatakeyama K., Nagashima T., Sugino T., Urakami K., Akiyama Y, & Yamaguchi K. (2023). Characterization of pancreatic cancer with ultra-low tumor mutational burden. Scientific Reports, 13, 4359.

+ Open protocol

+ Expand

Microarray Analysis of Tumor Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Eight samples (tumour n = 4, nontumour n = 4) were considered to have acceptable RNA quality for microarray analysis (RIN > 6·5, 260/280 and 260/230 > 1·8). From each sample, 25 ng of total RNA was used for the generation of amplified, fluorescent complementary RNA (cRNA), labelled with cyanine dye (Cy3) with the one‐color Low Input Quick Amp Labeling Kit (Agilent Technologies). The cRNA was purified using the RNeasy Mini Kit (Qiagen), and hybridized to SurePrint G3 Human Gene Expression 8x60K v2 Microarrays (Agilent Technologies) for 17 h at 65 °C before the fluorescent pattern was read with a G2565CA Microarray Scanner (Agilent Technologies). Analysis of the raw microarray images was performed using Agilent Feature Extraction software v10·7·3·1 (Agilent Technologies).

Tina E., Prosén S., Lennholm S., Gasparyan G., Lindberg M, & Göthlin Eremo A. (2018). Expression profile of the amino acid transporters SLC7A5, SLC7A7, SLC7A8 and the enzyme TDO2 in basal cell carcinoma. The British Journal of Dermatology, 180(1), 130-140.

+ Open protocol

+ Expand

Assessing Gene Expression in Radiation Exposure

Check if the same lab product or an alternative is used in the 5 most similar protocols

The effect of mixed neutron-photon exposure was studied on gene expression levels in peripheral blood of healthy individuals using Human Whole Genome Microarrays (4×44K V2; Agilent Technologies Inc., Santa Clara, CA). Total cellular RNA was isolated from blood samples 24 h postirradiation using QIAamp^® Blood RNA Mini Kit (QIAGEN^®, Valencia, CA). Globin-specific RNA was depleted from total RNA using a GLOBINclear Kit (Thermo Fisher Scientific™ Inc., Waltham, MA). RNA was quantified using the ND-1000 spectrophotometer (NanoDrop, Thermo Fisher Scientific) and quality was assessed using the Agilent Bioanalyzer. Good-quality RNA (100 ng; RIN > 8) was used for Cy3 labeling and amplification using the One-color Low Input Quick Amp Labeling Kit (Agilent Technologies). The labeled RNA were hybridized onto microarrays at 65°C in a hybridization oven (Agilent Technologies) and scanned using the Agilent DNA microarray scanner. The microarray data are available through the NCBI Gene Expression Omnibus (series no. GSE113611; https://bit.ly/2HLiQI8).

Mukherjee S., Grilj V., Broustas C.G., Ghandhi S.A., Harken A.D., Garty G, & Amundson S.A. (2019). Human Transcriptomic Response to Mixed Neutron-Photon Exposures Relevant to an Improvised Nuclear Device. Radiation research, 192(2), 189-199.

+ Open protocol

+ Expand

Transcriptomic Analysis of Human Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Transcriptomic analysis was performed using the SurePrint G3 Human GE v2 8×60K Microarray (Agilent Technologies, ID: 039494) according to the manufacturer’s protocol and to previously published [17 (link)]. Cyanine-3 labeled cRNAs were prepared using the One-Color Low Input Quick Amp Labeling kit (Agilent Technologies).
The slides were scanned using a G2565CA Scanner System (Agilent Technologies), and using a scan protocol with a resolution of 3 μm and a dynamic range of 20 bit. The resulting .tiff images were analyzed with the Feature Extraction Software v10.7.3.1 (Agilent Technologies) and using the GE2_107_Sep09 protocol. Original dataset was normalized using quantile method implemented in Limma’s package. Probes with bad detection p-values (<0.01) in half of the samples of a biological group were discarded. Ranked gene lists were obtained using statistical score of paired eBayes implemented in Limma’s package. Gene set enrichment analyses were performed using the Blood Transcription Modules [45 (link)] added with a selection of molecular signatures from C2 database provided by GSEA software [46 (link), 47 (link)]. Network analyses were performed using Cytoscape 2.8.3 and the plugin Enrichment Map dedicated to gene set enrichment analyses.
All datasets can be accessed in the ArrayExpress database (www.ebi.ac.uk/arrayexpress) under accession number E-MTAB-2858.

Rosenzwajg M., Churlaud G., Mallone R., Six A., Derian N., Chaara W., Lorenzon R., Long A., Buckner J., Afonso G., Pham H.P., Hartemann A., Yu A., Pugliese A., Malek T.R, & Klatzmann D. (2015). Low-dose interleukin-2 fosters a dose-dependent regulatory T cell tuned milieu in T1D patients. Journal of autoimmunity, 58, 48-58.

+ Open protocol

+ Expand

Comprehensive lncRNA and mRNA Profiling using Microarray

Check if the same lab product or an alternative is used in the 5 most similar protocols

A lncRNA microarray assay (Agilent-045142 Human SBC LncRNA v4 4 × 180K) was performed by Biotechnology Corporation (Shanghai, China). LncRNA and mRNA microarray analysis was performed as described previously [22 (link)]. Briefly, the total RNA content was amplified and transcribed into fluorescent cRNA using a one-color Low Input Quick Amp Labeling Kit (Agilent Technologies, Santa Clara, USA), and the labeled cRNA was purified using an RNeasy Mini Kit (QIAGEN) and hybridized to the Agilent Human SBC LncRNA Microarray (4 × 180K, platform: GPL18180, Agilent Technologies).
Approximately 30,000 lncRNAs were collected from authoritative data sources, including NCBI_refseq, NCBI_other, Ensembl, UCSC, LNCRNA-DB, Agilent, ncRNA-SCAN, and published papers. Moreover, the correlations between approximately 31,000 mRNAs and lncRNAs were analyzed using this array. After 17 hours of hybridization, slides were washed in staining dishes using a Gene Expression Wash Buffer Kit (Agilent Technologies), and all slides were scanned on an Agilent Microarray Scanner with the following default settings: 1) channel: green, 2) scan resolution: 3 μm, 20 bit. All data were extracted using Feature Extraction Software 10.7 (Agilent Technologies), and dates were normalized using a quantile algorithm via Gene Spring Software 11.0 (Agilent Technologies).

Min W., Dai D., Wang J., Zhang D., Zhang Y., Han G., Zhang L., Chen C., Li X., Li Y, & Yue Z. (2016). Long Noncoding RNA miR210HG as a Potential Biomarker for the Diagnosis of Glioma. PLoS ONE, 11(9), e0160451.

+ Open protocol

+ Expand

Transcriptomic Analysis of P. tricornutum

Check if the same lab product or an alternative is used in the 5 most similar protocols

200 ng of total RNA from three replicates randomly chosen from each treatment was reverse transcribed, amplified and labelled according to the One-Color Low Input Quick Amp Labeling Kit (Agilent; 5190–2305), using Cy3 as labelling dye. A total of 1,650 ng of cRNA from each sample was fragmented and hybridized with Gene Expression Hybridization Kit (Agilent; 5188–5242) on 4×44K P. tricornutum whole-genome 60-mer oligonucleotide microarrays (Agilent Technologies, Waldbronn, Germany) in an Agilent G2545A Hybridization Rotary Oven at 10 rpm, 65°C for 17.5 hours. Slides were washed with washing buffer 1 and 2 using Gene Expression Wash Buffer Kit (Agilent; 5188–5327) and directly scanned using a laser scanner (G2505 B; Agilent Technologies, Waldbronn, Germany) based on the “dynamic range expander” option in the scanner software. Images were processed by Agilent Feature Extraction software version 9.5.

Alipanah L., Winge P., Rohloff J., Najafi J., Brembu T, & Bones A.M. (2018). Molecular adaptations to phosphorus deprivation and comparison with nitrogen deprivation responses in the diatom Phaeodactylum tricornutum. PLoS ONE, 13(2), e0193335.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!