The following Abs were used in this study: anti‐Girdin (R&D Systems), anti‐Girdin (IBL), anti‐Girdin phospho S1647 (ECM Biosciences), anti‐histone H3 (1B1B2) (Cell Signaling Technology), anti‐histone H3 phospho S10 (Abcam), anti‐histone H3 phospho S28 (Abcam), anti‐cleaved PARP1 (Abcam), anti‐cleaved PARP1 (Cell Signaling Technology), anti‐Rb phospho Ser795 (New England BioLabs), anti‐Rb (4H1) (Cell Signaling Technology), anti‐p53 (Cell Signaling Technology), anti‐p53 phospho S15 (Cell Signaling Technology), anti‐p53 phospho S46 (Cell Signaling Technology), anti‐Mad2 (C‐10) (Santa Cruz Biotechnology), anti‐α‐tubulin (Sigma‐Aldrich), anti‐γ‐tubulin (Sigma‐Aldrich), Alexa Fluor 488 goat anti‐mouse IgG (Thermo Fisher Scientific), Alexa Fluor 488 goat anti‐rabbit IgG (Thermo Fisher Scientific), rabbit anti‐sheep IgG (H + L), Human SP ads‐HRP (Southern Biotech), and rabbit anti‐rat IgG H&L (HRP) (Abcam) Abs.
Anti cleaved parp 1
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-cleaved PARP-1 is a primary antibody that detects the cleaved form of Poly(ADP-ribose) Polymerase-1 (PARP-1) protein. PARP-1 is a key enzyme involved in the cell's DNA repair process. Cleavage of PARP-1 is a hallmark of apoptosis, or programmed cell death. This antibody can be used to monitor apoptosis in various experimental systems.
Automatically generated - may contain errors
Lab products found in correlation
Anti cleaved caspase 3, by Cell Signaling Technology (14 mentions)
Anti cleaved caspase 9, by Cell Signaling Technology (8 mentions)
Anti bcl 2, by Cell Signaling Technology (5 mentions)
Anti β actin, by Santa Cruz Biotechnology (5 mentions)
Heparin, by Merck Group (4 mentions)
Crystal violet, by Merck Group (4 mentions)
Endothelial cell growth supplement (ecgs), by Merck Group (4 mentions)
Calcein am, by Cayman Chemical (4 mentions)
Cm dcfda, by Cayman Chemical (4 mentions)
Anti caspase 3, by Enzo Life Sciences (4 mentions)
Liteablot turbo extra sensitive chemiluminescent substrate, by Euroclone (4 mentions)
Anti actin, by BD (4 mentions)
Anti akt, by Cell Signaling Technology (4 mentions)
Anti gapdh, by Santa Cruz Biotechnology (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Horseradish peroxidase hrp conjugated secondary antibody, by Agilent Technologies (3 mentions)
Anti cleaved caspase 7, by Cell Signaling Technology (3 mentions)
Anti caspase 7, by Cell Signaling Technology (3 mentions)
Anti caspase 3, by Cell Signaling Technology (3 mentions)
Anti p jak2, by Cell Signaling Technology (3 mentions)
28 protocols using anti cleaved parp 1
1
Comprehensive Protein Analysis Protocol
2
Western Blotting Protocol for Protein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Western blotting was performed as described previously [62 (link)]. The primary antibodies used in this study include anti-γH2A.X (1:1000, 2577S, Cell Signal Technology, Danvers, MA, USA), anti-p53 (1:500, sc-6243, Santa Cruz Biotechnology, Dallas, TX, USA), anti-p53ser15 (1:1000, 9286S, Cell Signal Technology, Danvers, MA, USA), anti-cleaved PARP1 (1:1000, 5625S, Cell Signal Technology, Danvers, MA, USA), anti-MCL1 (1:1000, 39224S, Cell Signal Technology, Danvers, MA, USA), anti-BCL2 (1:1000, 2872S, Cell Signal Technology, Danvers, MA, USA), anti-ACTIN (1:150,000, MAB1501, Millipore, Burlington, MA, USA). The secondary antibodies used were HRP-linked anti-rabbit IgG (1:4000, 7074S, Cell Signaling Technology, Danvers, MA, USA) and HRP-linked anti-mouse IgG (1:4000, 7076S, Cell Signaling Technology, Danvers, MA, USA).
3
Cell Apoptosis Assays and Western Blots
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All tissue culture reagents were from Euroclone, except ECGS and heparin (Sigma Aldrich). Crystal violet was from Sigma Aldrich and CM-DCFDA and calcein-AM from Cayman Chemicals. Primary antibodies used were mouse monoclonals anti-caspase-3 (Enzo Life Sciences, ALX-804-305) and anti-actin (BD Biosciences, 612656) and rabbit monoclonal anti-cleaved PARP-1 (Cell Signaling, #5625). Horseradish peroxidase (HRP)-conjugated secondary antibodies were from Dako (#P0260 and #P0399, for rabbit anti-mouse and swine anti-rabbit antibodies, respectively). Proteins in Western blot were detected by the LiteAblot Turbo Extra-Sensitive Chemiluminescent Substrate (Euroclone).
4
Western Blot Analysis of Apoptosis and Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We performed Western blotting according to the protocol described in a previous study [51 (link)]. The following antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA) for Western blotting: anti-cleaved Caspase3 (Cat#9661), anti-cleaved Caspase9 (Cat#9509), anti-cleaved PARP-1 (Cat#9541), anti-JAK1 (Cat# 3344), anti-p-JAK1 (Cat#74129), anti-STAT3 (Cat#9139), anti-p-STAT3 (Cat#9145), anti-p-PI3K (Cat#4228), anti-p-RAF1 (Cat#9427), anti-p-ERK (Cat#4370), anti-AKT (Cat#4691), and anti-p-AKT (Cat#4060). The anti-14-3-3-θ (Cat# ab183075) antibody was purchased from Abcam (Cambridge, UK). The anti-GAPDH (Cat#AC002) antibody was purchased from ABclonal Technology (Wuhan, China). HRP-conjugated anti-rabbit IgG (Cat#AS014) and HRP-conjugated anti-mouse IgG (Cat#AS003) (ABclonal Technology, Wuhan, China) were used as secondary antibodies, and enhanced chemiluminescence reagent (Vazyme, Nanjing, China) was used for detection of signals after exposure of the membrane in an image analyzer (Bio-Tanon, Shanghai, China).
5
Western Blot Analysis of EMT Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were lysed in lysis buffer. Equal amounts of total proteins were loaded onto 4-12% SDS–PAGE gels and transferred to PVDF membranes (GE Healthcare Life Sciences, NJ, USA). The membranes were blocked with 5% milk dissolved in TBS containing 0.02% Tween 20 and incubated overnight at 4°C with specific primary antibodies. The membranes were subsequently incubated with specific horseradish peroxidase-conjugated secondary antibodies. Protein bands were visualized using a Fusion FX5 system ((Vilber Lourmat, France). The following primary antibodies were used: anti-cleaved caspase 3, anti-E-cadherin, anti-cleaved caspase 9, anti-cleaved PARP1 (Cell Signaling Technology), anti-SNAIL1, anti-Vimentin, anti-Twist, anti-Slug, anti-Zeb1, anti-Nanog, anti-Sox2, anti-CD44 (Santa Cruz), anti-N-cadherin and anti-Oct4 (BD Biosciences), anti-Survivin, anti-CD133 (Abcam), anti-ALDH (Avivasysbio), and anti-β-actin (Sigma).
6
Antibody-based Protein Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Anti-phospho-DNA-PK, anti-cleaved PARP1, anti-cleaved caspase-3, anti-phospho-AMPK, anti-AMPK, anti-phospho-mTOR, anti-mTOR, anti-phospho-AKT and anti-AKT were all purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-vimentin and anti-b-Actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-γ H2AX was obtained from Millipore (Billerica, MA, USA). Metformin (1-(diaminomethylidene)-3, 3-dimethylguanidine) was purchased from Sigma-Aldrich Chemical Corp (St. Louis, MO, USA).
7
Western Blot Analysis of Cellular Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Western blot analyses were performed as previously described 28 (link). In brief, cells were lysed in whole-cell lysate buffer containing 1% phosphatase inhibitor cocktail and NPC tumor tissues were lysed in RIPA buffer (Beyotime, Jiangsu, China) with 1% phosphatase inhibitor cocktail. Lysates containing 20-30 µg protein were loaded onto 8% or 12% sodium dodecyl sulfate-polyacrylamide gels for electrophoresis (SDS-PAGE) and the separated proteins transferred to poly vinylidene fluoride (PVDF) membranes (Pall, NY, USA). After blocking with 5% fat free milk for 1 h in Tris-buffered saline (TBS), the membranes were incubated with the primary antibody overnight at 4°C and then with the peroxidase labelled secondary antibody (agilent, CA, USA) for 1 h on the next day. WB bands were visualized with an enhanced chemiluminescence kit (EMD Millipore, MA, USA). The primary antibodies used were γH2AX (1:2000 dilution, Epitomics, Burlingame, CA, USA) and monoclonal anti-cleaved PARP-1, β-actin, p-AKT, p-mTOR, SQSTM1/p62, LC-3, P-P65 (1:2000 dilution, Cell Signaling Technology, Danvers, MA, USA).
8
Western Blot Analysis of Apoptosis Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tumor tissue samples (100 mg) were homogenized in lysis buffer plus protease inhibitor tablet (Sigma-Aldrich). Protein concentration was determined using Bradford assay [9 (link)]. Sample proteins (45 μg) were loaded onto a range from 8–15 % Polyacrylamide Gel Electrophoresis and transferred onto nitrocellulose membrane by electro blotting under wet conditions (Mini Trans blot Bio-Rad). The membranes were incubated overnight at 4o C individually with the following antibodies: anti-p53, anti-Bcl-2, anti-Bax, anti-β-actin (Santa Cruz Biotechnology), anti-caspase-7, anti-cleaved caspase-7, anti-caspase-3, anti-cleaved caspase-3, anti-PARP-1, anti-cleaved PARP-1 (Cell Signaling Technology) at 1:1000 dilution. Then, they were incubated with their secondary antibody conjugated horseradish peroxidase (Santa Cruz Biotechnology) for two hours at room temperature at 1:6000 dilution. Then they were exposed to Kodak® film with chemiluminescent substrate (Super Signal System Pierce) and the resulting bands were analyzed and quantified by Image J® (National Institute of Health). β-actin was used as housekeeping.
9
Antibody-based Protein Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The antibodies used in the study were: anti-PLK1, anti-cleaved caspase-3, anti-cleaved PARP-1, anti-phospho-Histone H3 (Ser10) (Cell Signaling Technology); anti-vinculin (Sigma); anti-Cdc25A, anti-cyclin-B1 and -cyclin-D (Santa Cruz Biotechnology); anti-vinculin, anti-actin and anti-tubulin (Sigma); anti-RPA-2 (Neomarker, Union City, CS, USA), anti-γH2AX (Upstate Biotechnology, Lake Placid, NY, USA), anti-ubiquitin (Abcam, Cambridge, United Kingdom).
10
AZD1480 Cytotoxicity Evaluation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
AZD1480 was from MedChemExpress (Monmouth Junction, NJ, USA) and was dissolved in dimethyl sulfoxide (Sigma, St. Louis, MO, USA). The stock solution (40 mM) was stored at −20 °C. The 35~40% formaldehyde and Triton X-100 were from Sigma. Muse Cell Analyzer was from Merck Millipore (Billerica, MA, USA). FlowJo software to analyze raw data from Muse Cell Analyzer was from FlowJo LCC (version 10.5.3, Ashland, OR, USA). Primary antibodies in this study were as follows: anti-β-actin, anti-GAPDH, anti-Lamin A/C, anti-IL4Rα, and anti-IL13Rα1 were from Santa Cruz Biotechnology, and anti-cleaved PARP-1, anti-cleaved caspase-3, anti-FOXO3, anti-p27, anti-Bax, anti-Bcl-2, anti-JAK2, and anti-pJAK2 were from Cell Signaling Technology (Danvers, MA, USA). Horseradish peroxidase (HRP)-conjugated anti-rabbit and anti-mouse secondary antibodies were from Santa Cruz Biotechnology.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!