Routine urine analysis was done on an Urysis 2400 analyzer (Roche diagnostics) in the hospital core laboratory, using multi-parameter test cassette that measures pH, protein (albumin), glucose, ketones, bilirubin, urobilinogen, nitrite, erythrocyte, leucocyte esterase, and specific gravity. Only urine protein, erythrocyte, leucocyte esterase results that reflecte possible pre-existing nephropathy were recorded for data analysis in this study. The following cutoffs were used to classify results into test-positives. Urine protein positive: trace (0.25 g/L), 1+ (0.75 g/L), 2+ (1.5 g/L) and 3+ (5 g/L). Erythrocyte positive: trace (10Ery/μL), 1+ (25Ery/μL), 2+ (50Ery/μL), 3+ (150Ery/μL) and 4+ (250Ery/μL). Leucocyte esterase positive: trace (25Leu/μL), 1+ (100Leu/μL), 2+ (500Leu/μL). Urinary KIM-1 results were normalized to urine creatinine concentrations determined by analyzing all urine samples within 4 h of collection using an enzymatic assay on the Architect Ci8200 chemistry analyzer (Abbott Laboratories).
Architect ci8200 chemistry analyzer
Manufactured by Abbott
Sourced in United States
The Architect Ci8200 is a chemistry analyzer designed for clinical laboratory testing. It is capable of performing a wide range of clinical chemistry and immunoassay tests. The analyzer is equipped with advanced features to ensure accurate and reliable results.
Automatically generated - may contain errors
4 protocols using architect ci8200 chemistry analyzer
1
Routine Urinalysis and KIM-1 Measurement
2
Biochemical Assessment of Fasting Blood
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Venous blood samples were obtained from each subject after overnight fasting prior to and after the supplementation period. Blood samples were drawn in Vacutainer-type tubes containing a clot activator to determine biochemical parameters and then centrifuged for 30 min. The recovered sera were stored at −40 °C until further analysis for various biochemical parameters, including lactate dehydrogenase (LDH), creatine kinase (CPK), alkaline phosphatase (ALP), γ-glutamyl transferase (γ-GT), urea, creatinine, glutamic-oxaloacetic transaminase (SGOT), and glutamic-pyruvic transaminase (SGPT). All samples were analyzed by an external laboratory (Abbot Laboratories Architect ci8200 Chemistry Analyzer; P. Mantouvalou Diagnostic Laboratory AE, Athens, Greece).
3
Hematological and Echocardiographic Evaluation
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In all patients, RDW and all other hematological indices were measured, as part of the automated complete blood count (CBC), using a Cell-Dyn 3700 Hematology Analyzer (Abbott Diagnostics, Santa Clara, CA, USA). The reference range for RDW was 10–15 %. Biochemical analyzes were performed with the Architect ci8200 Chemistry Analyzer (Abbott Diagnostics, Santa Clara, CA, USA).
Transthoracic echocardiography was performed for each patient before surgery using a Vivid S3 (GE Healthcare, Milwaukee, WI, USA) with a 1.5–3.6 MHz phased array transducer. The left ventricular ejection fraction (LVEF) was measured using the modified Simpson’s rule [15 (link)].
Transthoracic echocardiography was performed for each patient before surgery using a Vivid S3 (GE Healthcare, Milwaukee, WI, USA) with a 1.5–3.6 MHz phased array transducer. The left ventricular ejection fraction (LVEF) was measured using the modified Simpson’s rule [15 (link)].
4
Comprehensive Blood and Cardiac Analysis
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Haematological indices were measured, as part of the automated complete blood count (CBC), using a Cell-Dyn 3700 haematology analyzer (Abbott Diagnostics, Santa Clara, CA, USA). Biochemical analyses were performed with the Architect ci8200 chemistry analyzer (Abbott Diagnostics, Santa Clara, CA, USA).
Transthoracic echocardiography was performed for each patient using a Vivid S3 (GE Healthcare, Milwaukee, WI, USA) with a 1.5-3.6 MHz phased array transducer.
Transthoracic echocardiography was performed for each patient using a Vivid S3 (GE Healthcare, Milwaukee, WI, USA) with a 1.5-3.6 MHz phased array transducer.
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