Mouse tnf alpha elisa kit

Vitamin C (Tianjin Kingyork, H12020392), Vitamin D3 (Beijing Solarbio Science & Technology, V8070), Mouse TNF-alpha ELISA Kit (RayBiotech, P06804), Mouse IL-6 ELISA Kit (RayBiotech, P08505), Mouse IL-1β ELISA Kit (Shanghai Tongwei Biotechnology, TW56961), Mouse insulin ELISA Kit (Shanghai Tongwei Biotechnology, TW085566), Mouse endotoxin (ET) ELISA Kit (Jiangsu Meimian, MM-0369M1), Total Cholestenone Content Assay Kit (Beijing Solarbio Science & Technology, BC 1985), Triglyceride Content Assay Kit (Beijing Solarbio Science & Technology, BC0625), Total SOD activity detection kit (Shanghai Beyotime Biotechnology, S0101S), Lipid oxidation detection kit (Shanghai Beyotime Biotechnology, S0131S), BCA protein concentration assay kit (Shanghai Beyotime Biotechnology, P0010), Oil red O (Sigma, O0625), Anti-FXR1 antibody (Abcam, ab155124), BSEP Monoclonal antibody (Proteintech Group, 67512-1-Ig), Anti-Fas antibody (Abcam, ab271016), Anti-ZO1 tight junction protein antibody (Abcam, ab221547), Anti-Occludin antibody (Abcam, ab222691), Anti-SLC10A2/ASBT antibody (Abcam, ab203205), TRIzol Reagent (Thermo Fisher, 15596026), cDNA synthesis kit (Beijing Tiangen Biotech, KR118), and SuperReal PreMix Plus (SYBR Green) (Beijing Tiangen Biotech, FP205) were used.

The serum levels of cytokines (IL-1β and TNF-α) were determined using the ELISA method. The amounts of IL-1β and TNF-α in the serum were determined using a mouse IL-1 beta ELISA Kit (RayBiotech, ELM-IL 1b, American) and a mouse TNF-alpha ELISA Kit (RayBiotech, ELM-TNFa, American), following the manufacturer’s instructions.

Cytokine quantification was measured following the manufacturer’s instructions, IFN-γ (RayBio® Mouse IFN-gamma ELISA Kit, Catalog #: ELM-IFNg); TNF-α (RayBio® Mouse TNF-alpha ELISA Kit, Catalog #: ELM-TNFa); IL-6 (RayBio® Mouse IL-6 ELISA Kit Catalog #: ELM-IL-6); IL-10 (ELISA assay using Ray Bio® ELISA Kits, Catalog #: ELM-IL-10). The concentration of the cytokines was determined by measuring color intensity at 450 nm using a microplate reader.