Milli q water from a

Manufactured by Merck Group

Sourced in United Kingdom, United States

Milli-Q water is a high-purity water system produced by Merck Group. It is designed to generate ultrapure water by removing impurities through a multi-stage purification process, including reverse osmosis, ion exchange, and ultrafiltration. The core function of the Milli-Q water system is to provide a consistent supply of highly purified water for various laboratory applications.

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3 protocols using milli q water from a

Anthocyanins, Phenolics, and Antioxidant Quantification

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In the present study, different chemicals for the MAE as well as for the quantification of anthocyanins, total phenolic compounds, and antioxidant activity have been employed as follows: methanol of HPLC purity (Fischer Chemical, Loughborough, UK), Milli-Q water from a Milli-Q water purification system (Millipore, Bedford, MA, USA), hydrochloric acid (Panreac, Barcelona, Spain), sodium hydroxide (Panreac, Barcelona, Spain), formic acid (Scharlau, Barcelona, Spain), anhydrous sodium carbonate (Panreac Química, Castellar del Valles, Barcelona, Spain), Folin–Ciocalteu reagent (Merck KGaA, EMD Millipore Corporation, Darmstadt, Germany), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) (Sigma-Aldrich, San Luis, MO, USA). Furthermore, for the quantification of anthocyanins, total phenolic compounds, and antioxidant activity, three standards were employed respectively: cyanidin chloride, gallic acid, and 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), all of them supplied by Sigma-Aldrich Chemical Co. (St. Louis, MO, USA).

González-de-Peredo A.V., Vázquez-Espinosa M., Espada-Bellido E., Ferreiro-González M., Carrera C., Barbero G.F, & Palma M. (2022). Extraction of Antioxidant Compounds from Onion Bulb (Allium cepa L.) Using Individual and Simultaneous Microwave-Assisted Extraction Methods. Antioxidants, 11(5), 846.

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Fabrication of Stimuli-Responsive Hydrogel Coatings

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Sodium salt of carboxymethylcellulose (CMC, M_w = 90 kDa, DS_COOH: 0.7) and poly(ethylene oxide) (PEG, M_w: 600 kDa) were purchased from Acros Organics (USA). 1,2,3,4-Butanetetracarboxylic acid (BTCA) was purchased from Merck (USA). Sodium hypophosphite monohydrate (SHP, NaPO₂H₂·H₂O) and diclofenac sodium salt (DCF) were purchased from Sigma-Aldrich (Germany). Quartz crystal microbalance (QCM) crystals coated with gold layer (QSX301) were purchased from LOT-Oriel, Germany. Milli-Q water from a Millipore water purification system (Millipore, USA; resistivity = 18.2 MΩ cm at 25 °C) was used for all sample preparation.

Kurečič M., Mohan T., Virant N., Maver U., Stergar J., Gradišnik L., Kleinschek K.S, & Hribernik S. (2019). A green approach to obtain stable and hydrophilic cellulose-based electrospun nanofibrous substrates for sustained release of therapeutic molecules. RSC Advances, 9(37), 21288-21301.

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Analytical Methods for Biomolecule Quantification

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All chemicals used in the experiment were of analytical reagent (AR) grade.
Creatinine, formate dehydrogenase, 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT), nicotinamide adenine dinucleotide (NAD + ), oxalate decarboxylase, 1-methoxy-5-methylphenazinium methyl sulfate (PMS), and urea [Co(NH2)2] were obtained from Sigma-Aldrich (St. Louis, MO). Monobasic sodium phosphate (NaH2PO4•2H2O), dibasic sodium phosphate (Na2HPO4), and magnesium sulfate (MgSO4•7H2O) were bought from Panreac (Barcelona, Spain). Ammonium chloride (NH4Cl), calcium chloride (CaCl2•2H2O), citric acid (C6H8O7•H2O), potassium chloride (KCl), sodium chloride (NaCl), sodium oxalate (Na2C2O4), tri-sodium citrate (Na3C6H5•2H2O) and uric acid were bought from Fisher Scientific (Pittsburgh, PA).
Sodium hydrogen carbonate (NaHCO3) was purchased from Merck (Darmstadt, Germany). Whatman No. 1 filter paper was bought from Cole-Parmer (Vernon Hills, IL). All glassware was thoroughly cleaned with freshly prepared 3:1 HCl/HNO3 and rinsed with Milli-Q water prior to use. NAD + was dissolved in phosphate buffer, pH 7.4, whereas sodium oxalate (Na2C2O4) was prepared using a citratephosphate buffer, pH 5.0. Milli-Q water from a Millipore system (R ≥ 18.2 MΩ cm -1 ) was used throughout the experiments.

Worramongkona P., Seeda K., Phansomboon P., Ratnarathorn N., Chailapakul O, & Dungchai W. (2018). A Simple Paper-based Colorimetric Device for Rapid and Sensitive Urinary Oxalate Determinations. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 34(1).

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