Tumor tissue was extracted, fixed in 10% formalin, embedded in paraffin, and sectioned (5 µm). Slides were stained with peroxidase-labeled antibodies to the CD3e (Santa Cruz Biotechnology), CD11b, Gr1 or CD34 cell surface markers (Novus Biologicals, Littleton, CO). The number of cells or tumor blood vessels identified by peroxidase substrate staining was counted using a Nikon Eclipse E600 with a 40×/1.0 NA Plan Apochromat oil objective (Nikon Instruments, Tokyo, Japan) and with Olympus DP-11 digital camera (Olympus America, Melville, NY). Images were processed using Meta-Morph 5.0.7 (Universal Imaging, West Chester, PA) and ImageJ (National Institutes of Health, Bethesda, MD) software.
Dp 11 digital camera
Manufactured by Olympus
Sourced in Germany
The DP-11 digital camera is a compact and lightweight device designed for laboratory and research applications. It features a high-resolution sensor that captures detailed images and supports various file formats. The camera is capable of delivering clear and accurate visual documentation for a wide range of scientific and analytical purposes.
Automatically generated - may contain errors
Lab products found in correlation
Cd11b, by Novus Biologicals (1 mentions)
Eclipse e600, by Nikon (1 mentions)
Ck40 inverted microscope, by Olympus (1 mentions)
Szx12 stereomicroscope, by Olympus (1 mentions)
Provis microscope, by Olympus (1 mentions)
Ccd camera, by Olympus (1 mentions)
Bx60 microscope, by Olympus (1 mentions)
Image pro software, by Media Cybernetics (1 mentions)
Alexa 488 conjugated anti mouse igg, by Thermo Fisher Scientific (1 mentions)
10 protocols using dp 11 digital camera
1
Quantification of Tumor Immune Cells
2
Pollen Characteristics in Rice Conditions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After seven days of treatments, all plants of each condition were in a flowering phenological stage. Plant height, number of inflorescences, and fresh weight of anthers of six plants (n = 6) per each condition were recorded. Plant height of each condition was recorded at 0 and day 7. Number of anthers were randomly counted from four plants (n = 4) per each condition. Pollen-containing anthers were manually removed from inflorescences undergoing anthesis using 75% (v/v) ethanol-sterilized forceps, and placed in 1.5 ml microcentrifuge tubes. The samples were stored at −80°C for further analyses.
Pollen grain counting, pollen morphology study, and pollen size measurement were performed on fresh pollen on the day of pollen collection. One milliliter of Calberla's solution as mounting media was added to stain pollen grains. The sample was placed on a glass slide and the microscopic observations were performed using the Olympus BX43 (Olympus Corp., PA, USA) compound microscope attached with the Olympus DP11 digital camera (Olympus Corp., PA, USA) at the magnification of 40x. To count pollen grains, an individual anther from four selected anther (n = 4) per each treatment was observed. Pollen morphology and pollen size were studied in rice only for the photographs. Length and width of 20 rice pollen grains (n = 20) per each sample group was measured using Image J (NIH Image, MD, USA).
Pollen grain counting, pollen morphology study, and pollen size measurement were performed on fresh pollen on the day of pollen collection. One milliliter of Calberla's solution as mounting media was added to stain pollen grains. The sample was placed on a glass slide and the microscopic observations were performed using the Olympus BX43 (Olympus Corp., PA, USA) compound microscope attached with the Olympus DP11 digital camera (Olympus Corp., PA, USA) at the magnification of 40x. To count pollen grains, an individual anther from four selected anther (n = 4) per each treatment was observed. Pollen morphology and pollen size were studied in rice only for the photographs. Length and width of 20 rice pollen grains (n = 20) per each sample group was measured using Image J (NIH Image, MD, USA).
3
Follicle Development Assessment Protocol
4
Skeletal Analysis of E18.5 Embryos
5
Fibrosis Evaluation in Hindlimb Muscles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Hindlimb muscle samples were fixed with 4% (w/v) paraformaldehyde in PBS at 4°C overnight, dehydrated and embedded in paraffin as described previously [25] . Sections (5 µm) were prepared, deparaffinized and stained with Sirius red for the fibrosis evaluation.
Microscopic observation and image acquisition were performed with a fluorescence microscope equipped with a DP-11 digital camera (Olympus, Hamburg, Germany). The percentage of muscle tissue occupied by fibrosis out of the total muscle tissue was calculated using FIJI (ImageJ) software (8 evenly distributed fields in 3 muscle sections were imaged per treatment).
Microscopic observation and image acquisition were performed with a fluorescence microscope equipped with a DP-11 digital camera (Olympus, Hamburg, Germany). The percentage of muscle tissue occupied by fibrosis out of the total muscle tissue was calculated using FIJI (ImageJ) software (8 evenly distributed fields in 3 muscle sections were imaged per treatment).
6
Histopathological Examination of Tumor Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The tumor masses were excised at the end of the experiment, cleaned with saline, and sectioned for histopathological examination. These sections were directly set in 10% neutral buffered formalin for 24 h and then subjected to a series of alcohol and xylene treatments. After that, the sections were fixed in paraffin wax and serially sectioned before being stained with hematoxylin and eosin. The inspection was performed using a light microscope (Olympus BX 51, Olympus America, Melville, NY) and photographed with Olympus DP11 digital camera connected to the microscope.55 (link)
7
Quantifying Hepatic Fibrosis and Lipid Deposition
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mouse liver tissues were fixed in 10% paraformaldehyde, embedded in paraffin wax, and thin sectioned (4 μm thickness) before staining. To quantify the hepatic fibrosis area, the prepared sections were stained with picro-sirius red and observed using an Olympus Provis microscope equipped with a CCD camera (Tokyo, Japan), as described previously [35 (link), 36 (link)]. For Oil Red O staining, liver sections were stained with 0.3% Oil Red O in triethyl phosphate in water (60% v/v) for 30 min [37 (link)]. The sections were then observed using a BX-60 microscope with a DP-11 digital camera (Olympus, GmbH, Hamburg, Germany). To ensure data accuracy and reliability, a minimum of 5 chicks was selected for analysis per group, at least 5 sections were studied per chick, and at least 7 randomly selected regions of interest (ROIs) per section were analyzed.
8
Myofiber Diameter Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Muscle sections were stained with H&E and the myofiber diameter was determined by analyzing the lesser diameters of the myofibers, as described by Dubowitz [47 ]. At least 10 arbitrary fields in two to three serial sections of each muscle sample from each mouse were photographed under a light microscope with a DP-11 digital camera (Olympus). Myofiber diameter was then determined with Adobe Photoshop software. In each muscle sample, the lesser myofiber diameter was measured for individual myofibers, analyzing between 4300 and 600 0 myofibers from four mice per treatment. Counted myofibers per section were averaged per animal and statistically analysed (n = 4).
9
Histological Assessment of Muscle Fibrosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Muscle samples were fixed with 4% paraformaldehyde in PBS at 4 °C overnight. They were then dehydrated and embedded in paraffin as previously described [22 (link)]. For fibrosis evaluation, the sections (5 μm) were stained with Sirius red as described in Turgeman et al. [22 (link)] and analyzed with ImagePro software (Media Cybernetics Inc., Silver Spring, MD, USA). Photographs (n = 20, i.e., 20 pictures from randomly selected sections from at least three different mice from each group of mice) were taken for analysis at 20× magnification under a light microscope (Olympus, Hamburg, Germany) with a DP-11 digital camera (Olympus). The results were calculated as red area divided by the total area (red + green) and presented as the proportion of fibrotic muscle area (mean ± SE). Blank areas, assumed to be artefactual, were excluded. There were no statistically significant differences between the mice in the same group. Therefore, all data of the same group were pooled.
10
Quantifying Immune Protein Expression in Spleen
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Paraffin-embedded spleen sections (CA, Hi-1, Hi-2) were treated with xylene, fixed in ethanol, washed, blocked with 5% goat serum or 2% BSA, and incubated at 4°C overnight with mouse-specific primary Abs for CDK9, IAP-1 and ISG-20. The slides were washed and further incubated for 2 h with secondary Abs conjugated to Alexa 555-, Alexa 350- and Alexa 488-conjugated anti-mouse IgG isotypes (1:100) (Invitrogen). After washing in PBS, the sections were air-dried and photographed with an Olympus IX-70 microscope (10X, 20X) equipped with a DP-11 digital camera (33 (link)). Fluorescence deposits from 10 microscopic fields of the tissue sections from 3 samples per group were counted. Mean numbers from each group were plotted for relative quantitation of the proteins.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!