129S2 mice were obtained from Charles River and C57BL/6.J (B6, CD45.2) and B6.SJL-Ptprca.Pep3b/BoyJ (CD45.1) were obtained from Taconic. Wnt5a+/− (Yamaguchi et al., 1999 (link)) mice obtained from The Jackson Laboratory were backcrossed to the (129S2 x C57BL/6.J)F1 (129B6, CD45.2) background for at least six generations. Age- and gender-matched Wnt5a+/+ (WT) littermates were used as controls in all experiments. In transplantation experiments, first-generation crosses of 129S2 and B6.SJL-Ptprca Pepcb/BoyJ (129Ly5.1, CD45.1xCD45.2) were used as recipients in intrinsic transplantation assays and as donors in extrinsic transplantations. All animal experiments were approved by the section Consumer Protection, Veterinary Services, and Food Hygiene of the Government of Upper Bavaria (Regierung Oberbayern, Munich, Germany). All animals were housed for at least a week before experimental use in microisolators under specific pathogen–free conditions, according to the Federation of Laboratory Animal Science Associations and institutional recommendations.
B6 sjl ptprca pep3b boyj
Manufactured by Charles River Laboratories
B6.SJL-Ptprca.Pep3b/BoyJ is a laboratory mouse strain that carries the Ptprca (CD45.1) allele. This allele is a useful marker for distinguishing between different cell populations in experimental settings.
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2 protocols using b6 sjl ptprca pep3b boyj
1
Wnt5a Heterozygous Mice Transplantation
2
Lineage Tracing in Mouse Embryos
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CD1, C57BL/6 J wild-type, B6.SJL-Ptprca Pep3b/BoyJ, SCID-Beige mice (Charles River Laboratories), Jag1−/− (ref. 30 (link)), β-actin-GFP (ref. 31 (link)) and ROSAeYFP (ref. 32 (link)) N1IP::CreLO (ref. 17 (link)) and N1IP::CreHI (ref. 19 (link)) strains were used. Animals were kept under pathogen-free conditions, and all procedures were approved by the Animal Care Committee of the Parc de Recerca Biomedica de Barcelona (regulation of Generalitat de Catalunya). Embryos were obtained from timed pregnant females and staged by somite counting: E10.5 (31–40 sp) E11.5 (43–48 sp). The detection of the vaginal plug was designated as day 0.5. Mice and embryos were genotyped using PCR when justified.
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