CT26 cells were obtained from Cell Resource Center, Chineses Academy of Medical Sciences, Beijing, China. Cells were cultured in RPMI 1640 medium (Sigma-Aldrich) supplemented with 10 % fetal bovine serum (FBS, Hyclone, USA), 100 U/ml penicillin, 100 μg/ml streptomycin, and 1 mM L-glutamine. Cultures were maintained at 37 °C with humidity and 5 % CO2.
BALB/c mice (female, 18-20 g body weight) were supplied by the Experimental Animal Center of the Fourth Military Medical University (FMMU) (Xi'an, China). In the experiment, 1 × 106 CT26 cells in 0.1 ml physiological saline were subcutaneously injected into the left oxter region of BALB/c mice. When the tumors grew to about 5 mm in diameter, mice were ready for experiments. All experiments using live animals were carried out with approval from the university's Institutional Animal Care and Use Committee.
BALB/c mice (female, 18-20 g body weight) were supplied by the Experimental Animal Center of the Fourth Military Medical University (FMMU) (Xi'an, China). In the experiment, 1 × 106 CT26 cells in 0.1 ml physiological saline were subcutaneously injected into the left oxter region of BALB/c mice. When the tumors grew to about 5 mm in diameter, mice were ready for experiments. All experiments using live animals were carried out with approval from the university's Institutional Animal Care and Use Committee.