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Human hprt1 4326321e

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The Human HPRT1 (4326321E) is a real-time PCR assay designed to detect the presence of the hypoxanthine phosphoribosyltransferase 1 (HPRT1) gene, which is a housekeeping gene expressed in human cells. This assay is intended for use in genetic research applications.

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Lab products found in correlation

Steponeplus real time pcr system, by Thermo Fisher Scientific (3 mentions) Stepone software v2, by Thermo Fisher Scientific (3 mentions) Trizol, by Thermo Fisher Scientific (3 mentions) Superscript 3 platinum one step qrt pcr kit, by Thermo Fisher Scientific (3 mentions)

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HPRT1 (47 citations)

3 protocols using human hprt1 4326321e

1

Quantitative Analysis of PTHLH and CASR

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RNA was isolated using TRIzol (Invitrogen). Quantitative RT-PCR was performed with the SuperScript III Platinum One-Step qRT-PCR Kit (Invitrogen) using a Step One Plus Real-Time PCR System (Applied Biosystems) and the following TaqMan primer sets: PTHLH, Hs00174969_m1; CASR, Hs01047795_m1; Pthlh, Mm00436057_m1; Casr, Mm00443375_m1. Human HPRT1 (4326321E) and mouse Actb (4352341E) were used as reference genes (Invitrogen). Relative mRNA expression was determined using the Step One Software v2.2.2 (Applied Biosystems).
Kim W., Takyar F.M., Swan K., Jeong J., VanHouten J., Sullivan C., Dann P., Yu H., Fiaschi-Taesch N., Chang W, & Wysolmerski J. (2016). Calcium-Sensing Receptor (CaSR) Promotes Breast Cancer by Stimulating Intracrine Actions of Parathyroid Hormone-Related Protein. Cancer research, 76(18), 5348-5360.
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2

Quantitative Expression Analysis of Membrane Proteins

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RNA was isolated using TRIzol (Invitrogen). Quantitative RT-PCR was performed with the SuperScript III Platinum One-Step qRT-PCR Kit (Invitrogen) using a Step One Plus Real-Time PCR System (Applied Biosystems) and the following TaqMan primer sets: flotillin1, Hs00195134_m1; MAL, Hs00707014_s1; MAL2, Hs01043579_u1; PMCA2, Hs01090447_m1; Ezrin, Hs00931646_m1; NHERF1, Hs00188594_m1. Human HPRT1 (4326321E) (Invitrogen) was used as a reference gene. Relative mRNA expression was determined using the Step One Software v2.2.2 (Applied Biosystems).
Jeong J., Shin J.H., Li W., Hong J.Y., Lim J., Hwang J.Y., Chung J.J., Yan Q., Liu Y., Choi J, & Wysolmerski J. (2021). MAL2 mediates the formation of stable HER2 signaling complexes within lipid raft-rich membrane protrusions in breast cancer cells. Cell reports, 37(13), 110160.
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3

Quantitative Expression Analysis of Membrane Proteins

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RNA was isolated using TRIzol (Invitrogen). Quantitative RT-PCR was performed with the SuperScript III Platinum One-Step qRT-PCR Kit (Invitrogen) using a Step One Plus Real-Time PCR System (Applied Biosystems) and the following TaqMan primer sets: flotillin1, Hs00195134_m1; MAL, Hs00707014_s1; MAL2, Hs01043579_u1; PMCA2, Hs01090447_m1; Ezrin, Hs00931646_m1; NHERF1, Hs00188594_m1. Human HPRT1 (4326321E) (Invitrogen) was used as a reference gene. Relative mRNA expression was determined using the Step One Software v2.2.2 (Applied Biosystems).
Jeong J., Shin J.H., Li W., Hong J.Y., Lim J., Hwang J.Y., Chung J.J., Yan Q., Liu Y., Choi J, & Wysolmerski J. (2021). MAL2 mediates the formation of stable HER2 signaling complexes within lipid raft-rich membrane protrusions in breast cancer cells. Cell reports, 37(13), 110160.
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