HEK293 cell viability was determined based on quantification of ATP using Cell Titer-Glo® luminescent Assay kit (Promega, Madison, WI, USA), which signals the presence of metabolically-active cells. Briefly, HEK293 cells were seeded into 96-well plates at a cell density of 3.5 × 104 cells per well, the plates were kept at 37 °C with 5% CO2 overnight. On the following day, cardamonin was added to the cells from a top concentration of 90 μΜ. The cells treated in the absence of the test compound were the negative control. After incubated for 24 h, Cell Titer-Glo reagent was added to the cells and Luminescence was acquired on EnVision™ 2100 Multilabel Reader (PerkinElmer, Santa Clara, CA, USA).
Envision 2100 multilabel reader
Manufactured by PerkinElmer
Sourced in United States
The EnVision™ 2100 Multilabel Reader is a microplate reader designed for automated detection and quantification of various assays, including fluorescence, luminescence, and absorbance. It features a compact design, advanced optics, and a user-friendly software interface to facilitate efficient data acquisition and analysis.
Automatically generated - may contain errors
7 protocols using envision 2100 multilabel reader
1
HEK293 Cell Viability Assay with Cardamonin
2
Cytotoxicity Evaluation of Dihydroartemisinin
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HEK293 cells were seeded at 10,000 cells/well into E-Plate and incubated at 37°C and 5% CO2. DH at different concentrations (0.32, 0.63, 1.25, 2.5, 5, 10, 20, 40 μL/mL) was added into each E-Plate well and incubated at 37°C and 5% CO2 for 24 h. Cell viability was detected by the addition of 100 μL CellTiter-Glo reagent (Promega, Madison, Wisconsin, USA) into each well containing cells in 100 μL medium and incubated for 10 minutes. Luminescence was read by Envision 2100 multilabel reader (PerkinElmer, Massachusetts, USA) [48 ]. Each experiment was performed with triplicates (Supplementary Figure 5A ). Furthermore, the viability of hiPS-CMs were evaluated with the cell index by RTCA cardio system (Supplementary Figure 5B ).
3
Caspase-Mediated Apoptosis Detection
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Apoptosis and cell death were detected using the Caspase-Glo Apoptosis Detection Kit according to manufactures’ instructions (Invitrogen), Briefly, Hela cells were seeded in 96-well plates at 10000 cells per well and incubate for 24 h at 37 °C, 5% CO2. Then, the medium was removed and washed with PBS, and followed with an additional incubation for 48 h in the presence of FBS-free medium. 100 μl of Caspase-Glo® 3/7 Reagent was added to each well and incubated for 30 min at RT. Luminescence of each sample was subsequently measured in a plate-reading luminometer (EnVision 2100Multilabel Reader, PerkinElmer).
4
Cell Proliferation Assay with CCK-8
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Cell proliferation was evaluated using a Cell Counting Kit-8 (CCK-8) (Promega, Madison, WI, USA). SKOV3 cells and A2780 cells were seeded in a 96-well culture plates at a density of 2×105 cells/well and incubated with different concentrations of the drugs (oxamate, AG14361 and olaparib) for different times. Cells treated in the absence of a test compound were the negative controls. Detection reagent was added to the cells, and the luminescence signals were determined with an EnVision™ 2100 Multilabel Reader (PerkinElmer, Santa Clara, CA, USA).
5
Fg Binding Inhibition Assay for L. lactis
6
Cell Proliferation Assay with Delfia
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LX-2 cells were seeded at a density of 20,000 cells/well in 96-well plates and then cultured for 48 h. The Dissociation-Enhanced Lanthanide Fluorescent Immunoassay (Delfia) Cell Proliferation Assay was performed following the manufacturer instructions (PerkinElmer, Waltham, MA, USA). The europium-labeled antibody was used to detect incorporated BrdU following Delfia Cell Proliferation Assay protocol. The dissociation of europium ions from the anti-BrdU antibody and the formation of their fluorescent chelates were obtained by DELFIA inducer reagent. The fluorescence, which is proportional to DNA synthesis, was measured by time-resolved fluorometer 2100 Envision™ Multilabel Reader (PerkinElmer).
7
LPS-Induced Cell Proliferation Assay
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LX-2 cells were seeded at a density of 6–8 × 103 in 96-well plates and then cultured for 3 and 24 h with or without LPS (100 and 500 ng/ml). The DELFIA (Dissociation-Enhanced Lanthanide Fluorescent Immunoassay) Cell Proliferation Assay was performed following the manufacturer instructions (PerkinElmer, MA, USA). Specifically, after LPS stimulus LX-2 cells were incubated with BrdU for 3 h. The europium-labeled antibody was used to detect incorporated BrdU following Delfia Cell Proliferation Assay protocol. The dissociation of europium ions from the anti-BrdU antibody and the formation of their fluorescent chelates were obtained by DELFIA inducer reagent. The fluorescence, which is proportional to DNA synthesis, was measured by time-resolved fluorometer 2100 Envision™ Multilabel Reader (PerkinElmer, MA, USA).
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