Guar gum, glutaraldehyde (25%), glycerol, dichloromethane, span-80, tween-20 were bought from Hi-Media Pvt. Ltd., Mumbai, India. A commercial fungicide (mancozeb) was purchased from the local dealer. The Vero cell line (derived from African green monkey kidney cells) used to mimic normal mammalian cells was preserved at National Research Center on Equines, Hisar. The Arun Hisar and Kufri Pukhraj variety of tomato and potato seeds, respectively, were supplied by the CCSHAU, Hisar vegetable division. Pathogenic fungi (ITCC3640, ITCC5431, ITCC5492, ITCC6343) were purchased from the Indian Type Culture Collection (ITCC), Division of Plant Pathology, IARI, New Delhi and were revitalized by purification.
Span 80
Manufactured by HiMedia
Sourced in India
Span 80 is a non-ionic surfactant commonly used as an emulsifier in laboratory settings. It is a clear, viscous liquid with a characteristic odor. Span 80 is often used to facilitate the formation and stabilization of emulsions, dispersions, and suspensions in various experimental protocols.
Automatically generated - may contain errors
Lab products found in correlation
Curcumin, by HiMedia (2 mentions)
Glutaraldehyde, by HiMedia (1 mentions)
Guar gum, by HiMedia (1 mentions)
Tween 20, by HiMedia (1 mentions)
Light liquid paraffin, by Loba Chemie (1 mentions)
Milli q water, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Rhodamine 123, by Merck Group (1 mentions)
Tween 80, by HiMedia (1 mentions)
Ceftazidime, by Merck Group (1 mentions)
Ceftazidime, by HiMedia (1 mentions)
Transcutol p, by HiMedia (1 mentions)
Propylene glycol, by HiMedia (1 mentions)
Hydroxypropyl methylcellulose, by HiMedia (1 mentions)
Soya lecithin, by HiMedia (1 mentions)
6 protocols using span 80
1
Fungal Biocontrol Agents Evaluation
2
Budesonide-Loaded Chitosan Nanoparticles
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Budesonide was obtained as a gift sample from Cipla Pvt. Ltd. Goa. Chitosan (medium molecular weight) and Cellulose acetate phthalate was procured from Yarrow Chemical Products, Mumbai, India. Light liquid paraffin procured from Loba Chemie Pvt. Ltd. Mumbai, India. Span 80 was procured from Hi-Media Laboratories Pvt. Ltd., Mumbai, India. Acetone and n-Hexane was procured from Molychem, Mumbai, India.
3
Preparation and Characterization of Acyclovir Formulations
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Acyclovir (ACV, 99.8% pure) was received as a gift from Cipla Pharmaceuticals (Mumbai, Maharashtra, India). Ethanol, methanol, and acetonitrile were HPLC (high performance liquid chromatography) grade, and these were purchased from Merck, Mumbai, Maharashtra, India. Buffer reagents (potassium dihydrogen phosphate, sodium chloride, and sodium hydroxide) were procured from S.D. Fine Mumbai, India. Carbopol 934 was purchased from Thane, Maharashtra, India. Rhodamine 123 was procured from Merck, Mumbai, India. Cholesterol (extra pure) was purchased from Sigma Aldrich, St. Louis, MO, USA. Phosphatidylcholine (PC) was purchased from Ludwinshafen, Germany. Propylene glycol (PG), tween 80, and span 80 were procured from Himedia Chemicals (Mumbai, India). In-house, installed distilled water was used as an aqueous solvent. For HPLC mobile phase preparation, Milli-Q water was used (Millipore, Billerica, MA, USA).
4
Antibacterial Synergy Evaluation of Ceftazidime and Curcumin
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Culture media reagents (Müeller Hinton Broth—MHB; Müeller Hinton agar—MHA), ceftazidime (CAZ), curcumin from Curcuma longa L. (CUR), and surfactants (Tween 80 and Span 80) were obtained from Himedia® and Sigma-Aldrich®. All solvents (Dimetilsulfoxide—DMSO, saline solutions) were purchased from Merck®.The products are as the following:
ceftazidime: Formula—C22H22N6O7S2, Molecular weight—636.65 g/mol, CAS number—78,439–06-2, concentration—100%). curcumin: Formula—C21H20O6, Molecular weight—368.38 g/mol, CAS number—458–37-7, Purity (HPLC)— ≥ 65%.
ceftazidime: Formula—C22H22N6O7S2, Molecular weight—636.65 g/mol, CAS number—78,439–06-2, concentration—100%). curcumin: Formula—C21H20O6, Molecular weight—368.38 g/mol, CAS number—458–37-7, Purity (HPLC)— ≥ 65%.
5
Primaquine-Loaded Transdermal Drug Delivery
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Primaquine was procured as a gift sample from Indian Pharmaceutical Combine Association Limited laboratories (IPCA laboratories, Ratlam, India). Castor oil I.P. (Indian Pharmacopeia) was purchased from a chemist shop (manufactured by Search Creation Company). Transcutol P (diethylene glycol monoethyl ether), propylene glycol, Span 80, and hydroxypropyl methylcellulose (HPMC) were procured from Himedia Laboratories Pvt. Ltd., Delhi, India. Tween 80 was procured from Ramagundam Fertilizers And Chemicals Limited (RFCL Ltd.) Delhi, India. Solvents, water, and other chemicals were used of analytical grade (chemicals that meet specifications of American Chemical Society (ACS) are called reagent or analytical grade). The animal experimental studies were approved by IAEC (Institutional Animal Ethical Committee) as per the regulation committee for CPCSEA (Committee for Purpose of Control and Supervision of Experiments on Animals) Government of India and registration number of approval was IAEC/JU/47.
6
Curcumin-Loaded Nanoparticle Formulation
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Curcumin was purchased on himedia lab, Mumbai, Soya Lecithin and span 80 purchase from HIMEDIA Lab Mumbai. Carbopol and PEG purchased from Loba Chem. Pvt. Ltd., Mumbai Tween 80 was purchase from HIMEDIA Laboratories Pvt. Ltd, Mumbai, All other chemicals were locally procured and solvents of merck grade were used.
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