miR-15b mimics (dsRNA oligonucleotides), miR-15b inhibitors (single-stranded oligonucleotides), and control oligonucleotides were ordered from GenePharma (Shanghai, China). The sequences are as follows: miR-15b mimics were 5′-CGAACCAUUAUUUGCUGCUUUA-3′ (sense), 5′-AAGCAGCAAAUAAUGGUUCGUU-3′ (antisense); negative control mimics were 5′-UUCUCCGAACGUGUCACGUTT-3′ (sense), 5′-ACGUGACACGUUCGGAGAATT-3′ (antisense); miR-15b inhibitors were 5′-TAAAGCAGCAAATAATGGTTCG-3′ (chemically modified by 2′-Ome); negative control inhibitors were 5′-CAGUACUUUUGUGUAGUACAA-3′.
Mir 15b mimic
Manufactured by GenePharma
Sourced in China
MiR-15b mimic is a synthetic double-stranded RNA molecule that mimics the function of the naturally occurring microRNA-15b. MicroRNAs are small, non-coding RNA molecules that play a role in the regulation of gene expression.
Automatically generated - may contain errors
Lab products found in correlation
Mir 15b inhibitor, by GenePharma (4 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Control oligonucleotide, by GenePharma (1 mentions)
Gsk 3β, by Santa Cruz Biotechnology (1 mentions)
Pcdna3.1 vector, by Thermo Fisher Scientific (1 mentions)
Mcl 1 sirna, by Santa Cruz Biotechnology (1 mentions)
Anti mir 15b, by GenePharma (1 mentions)
Caski, by American Type Culture Collection (1 mentions)
Mrc 5, by American Type Culture Collection (1 mentions)
Sirna, by Santa Cruz Biotechnology (1 mentions)
Oe nc, by RiboBio (1 mentions)
Inhibitor nc, by GenePharma (1 mentions)
Mimic nc, by GenePharma (1 mentions)
5 protocols using mir 15b mimic
1
Modulation of miR-15b Expression
2
Targeted Knockdown and Overexpression of GSK-3β and MCL-1 in NSCLC
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To knockdown the GSK-3β and MCL-1 directly, GSK-3β and MCL-1 siRNA were purchased from Santa Cruz Biotechnology (Santa Cruz, USA). To overexpress the GSK-3β and MCL-1 directly, the open reading frame of GSK-3β and MCL-1 without the 3'-UTR was amplified and cloned into the pcDNA3.1 vector (Life Technologies), respectively. miR-15b mimics (5'-UAGCAGCACAU-CAUGGUUUACA-3'), anti-miR-15b (5'-UGUAAAC-CAUGAUGUGCUGCUA-3') and negative control oligonucleotide (NCO, 5'-UGCACAGUUUAACCAGGAUUCA-3') were purchased from Genepharma Company (Shanghai, China). For transfection, plasmid (2 μg/mL) and RNA oligonucleotides (50 pmol/mL) were transfected into the NSCLC cells using Lipofectamine 2000 (Life Technologies) according to the manufacturer's instruction. Cells were collected and used for the following experiments 24 h after transfection.
3
Regulation of Cervical Cancer Cells
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A normal cervix epithelial Ect1/E6E7 cell and three cervical cancer cell lines HeLa, SiHa, and CaSki were obtained from American Tissue Culture Collection (ATCC, USA). All cells were cultured in RPMI-1640 (Hyclone, USA) containing 10% fetal bovine serum (FBS) in a humidified atmosphere with 5% CO2 at 37 °C.
MAGI2-AS3 overexpression vector (pEX-MAGI2-AS3) and the control vector were purchased from Ribobio (Guangzhou, China). The miR-15b mimic, miR-15b inhibitor, and the corresponding controls (NC) were synthesized by GenePharma (Shanghai, China). Lipofectamine 2000 (Invitrogen Life Technology) was used to transfect pEX-MAGI2-AS3, miR-15b mimic, and miR-15b inhibitor into HeLa cells. All cells were harvested at 48 h after transfection.
MAGI2-AS3 overexpression vector (pEX-MAGI2-AS3) and the control vector were purchased from Ribobio (Guangzhou, China). The miR-15b mimic, miR-15b inhibitor, and the corresponding controls (NC) were synthesized by GenePharma (Shanghai, China). Lipofectamine 2000 (Invitrogen Life Technology) was used to transfect pEX-MAGI2-AS3, miR-15b mimic, and miR-15b inhibitor into HeLa cells. All cells were harvested at 48 h after transfection.
4
Modulating miR-15b and BCL2 in LUAD
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The human LUAD cells PC9/ZD and SPC‐A1 cells, and bronchial epithelial cells MRC‐5 were purchased from the American Type Culture Collection (ATCC) (Manassas, VA, USA). All the cells were cultured in Dulbecco's modified Eagle's medium (DMEM: Gibco; Thermo Fisher Scientific, Waltham, MA, USA) and supplemented with 10% FBS (Gibco; Thermo Fisher Scientific) at 37°C in 5% CO2 with a humidified atmosphere of 95% air.
The miR‐15b mimic and miR‐15b inhibitor were purchased from GenePharma (Shanghai, China). BCL2 overexpressed plasmids (pcDNA3.1‐BCL2) obtained from the Chinese Academy of Sciences (Changchun, China). SPC‐A1 cells were utilized to perform the transfection using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA).
The miR‐15b mimic and miR‐15b inhibitor were purchased from GenePharma (Shanghai, China). BCL2 overexpressed plasmids (pcDNA3.1‐BCL2) obtained from the Chinese Academy of Sciences (Changchun, China). SPC‐A1 cells were utilized to perform the transfection using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA).
5
Targeting WWP1 and miR-15b in BMSCs
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Small interfering RNA (siRNA) targeting WWP1 and negative control (NC) of siRNA (both from Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA) were transfected into BMSCs under the mediation of DharmaFECT one transfection reagent (Thermo Scientific, Lafayette, CO, USA, www.dharmacon.com ).
Based on the manuals provided by Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA), lentiviral vectors carrying short hairpin RNA (sh)-NC, sh-KLF2, overexpression (oe)-NC, oe-KLF2, and oe-WWP1 (Ribobio, Guangzhou, Guangdong, China) were transduced into BMSCs. Following 6 h, the medium was renewed, and cell culture was further conducted for 48 h.
Mimic-NC (100 nM), miR-15b mimic (100 nM), inhibitor-NC (100 nM) and, miR-15b inhibitor (100 nM) (Shanghai GenePharma Co., Ltd., Shanghai, China) were transfected into BMSCs by using Lipofectamine 2000 (Invitrogen). After 48 h of transfection, reverse transcription quantitative polymerase chain reaction (RT-qPCR) was performed to assess the transfection efficiency.
Based on the manuals provided by Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA), lentiviral vectors carrying short hairpin RNA (sh)-NC, sh-KLF2, overexpression (oe)-NC, oe-KLF2, and oe-WWP1 (Ribobio, Guangzhou, Guangdong, China) were transduced into BMSCs. Following 6 h, the medium was renewed, and cell culture was further conducted for 48 h.
Mimic-NC (100 nM), miR-15b mimic (100 nM), inhibitor-NC (100 nM) and, miR-15b inhibitor (100 nM) (Shanghai GenePharma Co., Ltd., Shanghai, China) were transfected into BMSCs by using Lipofectamine 2000 (Invitrogen). After 48 h of transfection, reverse transcription quantitative polymerase chain reaction (RT-qPCR) was performed to assess the transfection efficiency.
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