Dionex ase 350 system

Manufactured by Thermo Fisher Scientific

Sourced in United States, Germany

The Dionex ASE 350 system is an automated solvent extraction instrument used for the efficient extraction of analytes from solid and semi-solid samples. The system employs accelerated solvent extraction technology to rapidly extract compounds from a variety of sample matrices using small volumes of organic solvents or water.

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Lab products found in correlation

Centrifuge 5810r, by Eppendorf (1 mentions) Diatomaceous earth, by Thermo Fisher Scientific (1 mentions) Gc chemstation software, by Agilent Technologies (1 mentions) Omegawax capillary column, by Merck Group (1 mentions)

5 protocols using dionex ase 350 system

Pea Extract Isolation Protocol

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To extract different parts of the pea, a Dionex ASE 350 system (Thermo
Scientific, Waltham, MA, USA) was used, equipped with an autosampler
carousel and a collecting tray for the sequential extraction of 24
samples. A 10 mL stainless steel extraction cell containing 1 g of
the test material and glass beads was used, and 96% ethanol was used
as the solvent for extraction. The extraction was carried out at 50
°C, 10 MPa pressure, for a static time of 10 min, with 3 static
cycles. The extracts were centrifuged (4000× g) (Eppendorf TM Centrifuge 5810R, Hamburg, Germany) and stored at
−20 °C until further analysis.

Walczak-Skierska J., Krakowska-Sieprawska A., Monedeiro F., Złoch M., Pomastowski P., Cichorek M., Olszewski J., Głowacka K., Gużewska G, & Szultka-Młyńska M. (2024). Silicon’s Influence on Polyphenol and Flavonoid Profiles in Pea (Pisum sativum L.) under Cadmium Exposure in Hydroponics: A Study of Metabolomics, Extraction Efficacy, and Antimicrobial Properties of Extracts. ACS Omega, 9(13), 14899-14910.

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Optimized Herb Extraction Using ASE

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Extraction of aerial parts
of herbs was accomplished using a Dionex ASE 350 system (Thermo Scientific,
Waltham, MA, USA) equipped with an auto-sampler carousel and a collection
tray. The sample (15 g) of ground dried herbs was mixed with diatomaceous
earth (Thermo Scientific) in 100 mL of extraction cell. The following
conditions were used for the extraction: solvent—50% ethanol,
temperature—80 °C, pressure—1500 psi, static time—10
min, and static cycle—3.

Paun G., Neagu E., Albu C., Savin S, & Radu G.L. (2020). In Vitro Evaluation of Antidiabetic and Anti-Inflammatory Activities of Polyphenolic-Rich Extracts from Anchusa officinalis and Melilotus officinalis. ACS Omega, 5(22), 13014-13022.

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Pressurized Ethanol Extraction of Samples

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The method was adopted from Arapitsas and Turner [24] (link). Briefly, approximately 1 g of freeze-dried sample was weighed. The samples were extracted using a Dionex ASE-350 system (Thermo Fisher, Germering, Germany). The solvent used was water/ethanol/formic acid (94/5/1v/v/v) pressurized at 103 bar. The extraction was carried out with two cycles at a temperature of 99 °C with a preheating time of 6 min and extraction time of 10 min.

Gondo T.F., Huang F., Marungruang N., Heyman-Lindén L, & Turner C. (2024). Investigating the quality of extraction and quantification of bioactive compounds in berries through liquid chromatography and multivariate curve resolution. Analytical and bioanalytical chemistry.

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Accelerated Solvent Extraction of Medicinal Plants

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Accelerate solvent extraction of dried and grounded M. sativa and S. virgaurea was realized by Dionex ASE 350 System (Thermo Fisher Scientific Inc., Waltham, MA USA). Each stainless steel cell (100 mL) equipped with a cellulose filter was filled with 15 g of dried and fine-milled plant and diatomaceous earth and the ASE conditions were set as solvent—ethanol/water (50/50, v/v), temperature—60 °C, static time—10 min, number of cycles—3. The extracts were collected in a 250 mL flask and stored at 4 °C before concentration. According to the ASE extract volume, the concentration of the extracts was 9% (w/v).

Paun G., Neagu E., Alecu A., Albu C., Seciu-Grama A.M, & Radu G.L. (2024). Evaluating the Antioxidant and Antidiabetic Properties of Medicago sativa and Solidago virgaurea Polyphenolic-Rich Extracts. Molecules, 29(2), 326.

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Milk Lipid Extraction and Fatty Acid Analysis

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Milk lipids were determined with accelerated solvent extraction method using Dionex ASE 350 system (Thermo Scientific, Dreieich, Germany) with petroleum ether in isopropanol (2:1) as solvent. Methyl esterification of FA was carried out according to Palmquist and Jenkins (2003) with a basic or acid reaction. Fatty acid separation and quantification were performed by a Agilent 7820A GC System equipped with an automatic sampler G4567A (Agilent Technologies, Santa Clara, CA) and flame ionization detector. The column used was a Supelco Omegawax capillary column (30 m in length, 0.25-mm inner diameter and a film thickness of 0.25 μm; Supelco, Bellefonte, PA). Temperatures of injector and flame ionization detector were set at 250°C. Oven temperature was initially 50°C for 2 min, increased at 4°C/min to 220°C, and held for 18 min. Hydrogen was the carrier gas and its flow was set at 1 mL/min with average speed of 21 cm/s. Fatty acid standard Supelco FAME mixC4-C24 #18919-1AMP (Sigma-Aldrich, Castle Hill, Australia) was analyzed before GC analysis for FA identification. Determination of FA values was obtained using GC ChemStation software (Agilent Technologies, Santa Clara, CA) and were expressed both as total identified FA and on a milk basis. Individual FA were C4:0, C6:0, C8:0, C12:0, C14:0, C16:0, C16:1n-7, C18:0, and C18:1n-9, and groups of FA were SFA, UFA, MUFA, and PUFA.

Gottardo P., Penasa M., Lopez-Villalobos N, & De Marchi M. (2016). Variable selection procedures before partial least squares regression enhance the accuracy of milk fatty acid composition predicted by mid-infrared spectroscopy. Journal of dairy science, 99(10).

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