Gas chromatographic mass spectrometry

Manufactured by Thermo Fisher Scientific

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Gas chromatographic mass spectrometry (GC-MS) is an analytical technique that combines gas chromatography and mass spectrometry to identify and quantify chemical compounds in a sample. The gas chromatograph separates the sample components, while the mass spectrometer detects and measures the molecular weight of each component.

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Lab products found in correlation

Radioimmunoassay, by Merck Group (2 mentions) Access assay, by Beckman Coulter (2 mentions)

2 protocols using gas chromatographic mass spectrometry

Measuring Plasma Glucose Metabolism

Cited 1 time

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Plasma samples were placed on ice, centrifuged at 4°C, separated, and stored at −20°C until assayed. Glucose concentrations were measured using a glucose oxidase method (Yellow Springs Instruments, Yellow Springs, OH, USA). Plasma insulin was measured using a chemiluminescence assay (Access Assay; Beckman Coulter, Chaska, MN, USA). Plasma glucagon and C-peptide were measured by Radio-Immunoassay (EMD Millipore, Billerica, MA, USA). Plasma [6,6-²H₂] glucose and [1-¹³C] glucose enrichments were measured using gas chromatographic mass spectrometry (Thermoquest, San Jose, CA, USA) to simultaneously monitor the C-1 and C-2 and C-3 to C-6 fragments, as described by Beylot et al.23 (link) In addition, [6-³H] glucose specific activity was measured by liquid scintillation counting following deproteinization and passage over anion and cation exchange columns.22 (link)

Sathananthan M., Ikramuddin S., Swain J.M., Shah M., Piccinini F., Dalla Man C., Cobelli C., Rizza R.A., Camilleri M, & Vella A. (2014). The effect of vagal nerve blockade using electrical impulses on glucose metabolism in nondiabetic subjects. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 7, 305-312.

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Measurement of Glucose Metabolism Markers

Cited 1 time

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Plasma samples were placed on ice, centrifuged at 4°C, separated, and stored at −20°C until assayed. Glucose concentrations were measured using a glucose oxidase method (Yellow Springs Instruments, Yellow Springs, OH). Plasma insulin was measured using a chemiluminescence assay (Access Assay; Beckman, Chaska, MN). Plasma glucagon and C-peptide were measured by Radio-Immunoassay (EMD Millipore, Billerica, MA). Collection tubes for GLP-1 had 100 μM of DPP-4 inhibitor added. Total GLP-1 concentrations were measured using a C-terminal assay (EMD Millipore, Billerica, MA). Plasma [6,6-²H₂] glucose and [1-¹³C] glucose enrichments were measured using gas chromatographic mass spectrometry (Thermoquest, San Jose, CA) to simultaneously monitor the C-1 and C-2 and C-3 to C-6 fragments, as described previously [19 (link)]. In addition, [6-³H] glucose specific activity was measured by liquid scintillation counting following deproteinization and passage over anion and cation exchange columns [18 (link)].

Shah M., Laurenti M.C., Man C.D., Ma J., Cobelli C., Rizza R.A, & Vella A. (2018). Contribution of Endogenous Glucagon-Like Peptide-1 to Changes in Glucose Metabolism and Islet Function in People with Type 2 Diabetes Four Weeks after Roux-en-Y Gastric Bypass (RYGB). Metabolism: clinical and experimental, 93, 10-17.

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