2-Methoxyestradiol (2ME2) was purchased from Selleck (Houston, TX, USA). RPMI 1640, Ham's F-12K and fetal bovine serum were purchased from Gibco/BRL Life Technologies (Grand Island, NY, USA). Anti-HIF-1α primary antibodies were purchased from BioWorld (St Louis Park, MN, USA). Lamin B1 antibodies were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Anti-β-actin primary antibodies were obtained from Cell Signaling Technology (Boston, MA, USA). Their respective horseradish peroxidase (HRP)-conjugated secondary antibodies were purchased from Beyotime (Shanghai, China). PVDF membranes were purchased from Bio-Rad (Richmond, CA, USA). The enhanced chemiluminescence (ECL) agent was obtained from Thermo Fisher Scientific (Waltham, MA, USA). An Immunol Fluorescence Staining Kit with Alexa Fluor 555-Labeled Donkey Anti-Rabbit IgG and 2-(4-amidinophenyl)-6-indolecarbamidine (DAPI) were obtained from Beyotime (Shanghai, China). TRIzol was purchased from Invitrogen (Grand Island, NY, USA), and One Step PrimeScript RT-PCR Kit was obtained from TaKaRa (Dalian, Liaoning, China).
2 methoxyestradiol 2me2
Manufactured by Selleck Chemicals
Sourced in United States
2-Methoxyestradiol (2ME2) is a chemical compound used in research applications. It is a naturally occurring metabolite of the hormone estradiol. 2ME2 functions as an inhibitor of angiogenesis, the process of new blood vessel formation, which is relevant in certain research areas.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
One step primescript rt pcr kit, by Takara Bio (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Horseradish peroxidase hrp conjugated secondary antibody, by Beyotime (1 mentions)
Anti β actin primary antibody, by Cell Signaling Technology (1 mentions)
Pvdf membrane, by Bio-Rad (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Ham s f 12k, by Thermo Fisher Scientific (1 mentions)
2 4 amidinophenyl 6 indolecarbamidine dapi, by Beyotime (1 mentions)
Bicinchoninic acid bca protein assay kit, by Beyotime (1 mentions)
3 protocols using 2 methoxyestradiol 2me2
1
Molecular Mechanisms of 2ME2 Inhibition
2
Neuroprotective Effects of Dexamethasone and Rapamycin in Ischemic Stroke
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DEX (Jiangsu Hengrui Medicine Co., Ltd., China; Gu et al., 2011 (link)), and rapamycin (RAPA, Sigma) were diluted or dissolved with 0.9% saline, then intraperitoneally injected into mice at dosages of DEX (25 μg/kg) and RAPA (80 μg/kg). 3-MA, Sigma-was dissolved in 0.9% saline and was injected intracerebroventricular into mice with 3 μl of a 20 mg/ml (Wang et al., 2014 (link)). At the onset of the reperfusion, the treatment groups, including tMCAO+DEX, tMCAO+3-MA, tMCAO+DEX+3-MA, tMCAO+RAPA and tMCAO+DEX+RAPA were administrated with DEX, 3-MA, DEX combined with 3-MA and RAPA and DEX combined with RAPA respectively. 2-Methoxyestradiol (2ME2, Selleck) was dissolved in 0.5% dimethylsulfoxide (DMSO) and intraperitoneally injected 30 min after tMCAO (Schaible et al., 2014 (link)). Sham and tMCAO groups without drug treatment were injected with the same volume of 0.9% saline or DMSO.
3
Hypoxia-Induced Autophagy Regulation
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High glucose Dulbecco's modified Eagle's medium (DMEM/H-G) was purchased from Hyclone Laboratories; GE Healthcare Life Sciences (Logan, UT, USA). Fetal bovine serum (FBS) was purchased from Gibco; Thermo Fisher Scientific, Inc. (Waltham, MA, USA). The 4′,6-diamidino-2-phenylindole (DAPI) 0.25% trypsin-EDTA, Cell Counting Kit (CCK-8), protein extraction kit including radioimmunoprecipitation assay buffer (RIPA), and bicinchoninic acid (BCA) protein assay kit were purchased from Beyotime Institute of Biotechnology (Haimen, China). Dimethyl sulfoxide (DMSO) and bovine serum albumin (BSA) were purchased from Biosharp Biotechnology Co. (Hefei, China). 2-Methoxyestradiol (2ME2) was purchased from Selleck Chemicals (Houston, TX, USA). Antibodies against HIF-1α (cat. no. ab179483), microtubule-associated protein 1A/1B-light chain 3 (LC-3) phosphatidylethanolamine conjugate (LC3-II) (cat. no. ab51520), and Beclin-1 (cat. no. ab207612) were obtained from Abcam (Cambridge, UK). Antibodies against β-actin (cat. no. GB13001-3), and tetramethylrhodamine (TRITC)-conjugated secondary antibody (cat. no. 111-095-003) were obtained from Sevicebio Technology (Wuhan, China).
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