Doxorubicin (Dox) hydrochloride was purchased from Hisun Pharmaceutical Co. Ltd., (Zhejiang, China). Chlorin e6 (Ce6) was obtained from J&K Scientific Ltd., (Shanghai, China). Monodisperse Mesoporous Silica Nanosphere-Stellate MSNs (MSNs) was purchased from XFNano (Nanjing, China). Trypsine-EDTA, fetal bovine serum (FBS) and phosphate buffered solution (PBS) were purchased from Gibco-BRL (Burlington, Canada). The RPMI 1640 medium and hoechst 33342 were obtained from Yeasen (Shanghai, China). Singlet oxygen sensor green reagent, propidium iodide (PI), Reactive oxygen species assay kit and LIVE/DEAD Viability/Cytotoxicity Kit were purchased from Invitrogen (Oregon, USA). Protease inhibitor cocktail (EDTA-Free, 100× in DMSO) was obtained from SelleckChem (Houston, USA). Other chemicals if not mentioned were analytical grade and obtained from Sinopharm Group Chemical Reagent Co., Ltd. (Shanghai, China). Ultrapure water was produced with a Milli-Q water purification system. All glassware was rinsed extensively with ultrapure water and dried before use.
Chlorin e6 ce6
Manufactured by J&K Scientific
Sourced in China
Chlorin e6 (Ce6) is a photosensitizing agent used in various scientific applications. It is a naturally occurring chlorophyll derivative with the chemical formula C₃₃H₃₆N₄O₆. The core function of Chlorin e6 is to absorb light and generate reactive oxygen species, which can be utilized in various research and experimental settings.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Hoechst 33342, by Yeasen (2 mentions)
Propidium iodide, by Thermo Fisher Scientific (1 mentions)
Singlet oxygen sensor green reagent, by Thermo Fisher Scientific (1 mentions)
Trypsine edta, by Thermo Fisher Scientific (1 mentions)
Live dead viability cytotoxicity kit, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Yeasen (1 mentions)
Griess reagent, by Beyotime (1 mentions)
Atp assay kit, by Beyotime (1 mentions)
Jc 1 assay kit, by Beyotime (1 mentions)
Singlet oxygen sensor green (sosg), by Thermo Fisher Scientific (1 mentions)
Polyvinyl alcohol (pva), by Merck Group (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions)
Hypoxia detection kit, by Enzo Life Sciences (1 mentions)
Calcein am, by Dojindo Laboratories (1 mentions)
2 7 dichlorodihydrofluorescein diacetate dcfh da, by Beyotime (1 mentions)
3 amino 4 aminomethyl 2 7 difluorescein diacetate daf fm da, by Beyotime (1 mentions)
Sodium dithionite na2s2o4, by Merck Group (1 mentions)
Cell counting kit 8 cck 8, by Keygen Biotech (1 mentions)
Hydrogen peroxide h2o2, by Merck Group (1 mentions)
7 protocols using chlorin e6 ce6
1
Optimized Photodynamic Therapy Using MSNs
2
PLGA-Based Photodynamic Therapy for Hypoxic Tumors
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Biocompatible poly-lactic-co-glycolic acid (50:50, PLGA, MW: 12,000 Da) was obtained from Shandong Key Laboratory of Medical Polymer Materials (Shandong, China). Chlorin e6 (Ce6) was from J&K (Beijing, China) and L-Arginine from Aladdin-Reagent Ltd., Co. (Shanghai, China). Polyvinyl alcohol (PVA) was purchased from Sigma-Aldrich Chemical Co. (St Louis, USA). Other reagents kits were: chloroform (CHCl3) and isopropyl alcohol (Chongqing East Chemical Industry Ltd., Co., Chongqing, China), Singlet Oxygen Sensor Green (SOSG) (Invitrogen, Massachusetts, USA), hypoxia detection kit (Enzo Life Sciences Inc., Farmingdale, USA), Complex IV activity assay kit (Solarbio Science &Technology Ltd., Co., Beijing, China). Calcein-AM, propidium iodide (PI) and CCK-8 assays were from Dojindo Laboratories (Kumamoto, Japan). 3-Amino,4-aminomethyl-2',7'-difluorescein, diacetate (DAF-FM DA), Griess Reagent, JC-1 assay kit, ATP assay kit, 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA), 4,6-diamidino-2-phenylindole (DAPI) were all obtained from Beyotime Biotechnology Ltd., Co. (Shanghai, China).
3
Photosensitizer-Loaded Nanoparticle Synthesis
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Human hemoglobin (Hb), gadolinium chloride hexahydrate (GdCl3·6H2O), and sodium dithionite (Na2S2O4) were obtained from Sigma Aldrich (USA). Chlorin e6 (Ce6) was acquired from J&K Scientific Ltd. (China). N-Hydroxylsuccinimide-derivatized methoxy-poly (ethylene glycol) (mPEG-NHS, MW = 2000 Da) was purchased from Thermo Fisher Scientific (USA). Sodium hydroxide (NaOH), borate saline buffer (BBS, pH 8.2) and phosphate-buffered saline (PBS, pH 7.4) were obtained from Sinopharm. Cell Counting Kit-8 (CCK-8) was purchased from KeyGEN bioTECH (Nanjing, China).
4
Chitosan-Catalase-Photodynamic Therapy
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Chitosan (low molecular weight, 75.0% deacetylation), Catalase, hydrogen peroxide (H2O2), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT), and formaldehyde were obtained from Sigma-Aldrich (St. Louis, MO, USA). Chlorin e6 (Ce6) (MW = 596.68, 93-98%) was purchased from J&K Scientific Co (Shanghai, China). 9,10-Diphenylanthracene (DPA) was supplied by Alfa Aesar Co. (Shanghai, China). Dye Hoechst nuclear agent, AnnexinV-FITC apoptosis kit, and 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) were purchased from Life Technologies Co. DMEM high glucose medium (HyClone), fetal bovine serum (HyClone), Penicillin-Streptomycin Solution (HyClone), 0.25% trypsin (containing 0.02% EDTA), and phosphate-buffer saline (PBS) were purchased from Corning Costar Co. (Shanghai, China). Ultrapure water was prepared with a Millipore Simplicity System (Millipore, Bedford, USA).
5
Comprehensive Cellular Assay Protocol
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Hemoglobin (Hb), 9,10-Anthracenediyl-bis(methylene) dimalonic acid (ABDA), and bis(2,4,5-trichloro-6-carbopentoxyphenyl) oxalate (CPPO) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Lactate oxidase (LOX) was purchased from Shanghai Yuanye Bio-Technology (Shanghai, China). Chlorin e6 (Ce6) was purchased from J&K Scientific Ltd (Beijing, China). RPMI 1640 medium, Dulbecco’s modified Eagle’s medium (DMEM), and fetal bovine serum (FBS) were obtained from Gibco Life Technologies (AG, Switzerland). Rhodamine phalloidin was obtained from Invitrogen (USA). Trypsin-EDTA and penicillin-streptomycin, were purchased from Corning life sciences (Wujiang, China). Hoechst 33342, calcein-AM/propidium iodide (PI), Apoptosis Analysis Kit, and 2′,7′-dichlorofluorescin diacetate (DCFH-DA) were purchased from Solarbio® life sciences (Beijing, China). Cell cycle Analysis Kit was obtained from Beyotime (Beijing, China). The antibodies and corresponding dilution ratio were listed in Supplementary Table 2 . The siRNA sequences (Sangon Biotech, Shanghai) were listed in Supplementary Table 3 . pcDNA3.1-MEF2C (KWC20220217AZGYQ-PC01) and pcDNA3.1-NAMPT (KWC20220217AZGYQ-PC02) were constructed by Hanbio Biotech (Shanghai, China).
6
Synthesis and Characterization of Photosensitizer-loaded Nanoparticles
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Methoxy polyethylene glycols (mPEG, 2 kDa), 2,2′-dipyridyl disulfide (DPDS), 2-(2-hydroxyethyl) pyridine, methacryloyl chloride, and chlorin e6 (Ce6) were purchased from J&K Scientific Ltd (Beijing, China). Carbon disulfide (CS2), 1-dodecanethiol (C12H26S), 18-crown 6-ether (C12H24O6) and triethylamine (TEA) were purchased from Aladdin Co. (China). 2-Mercaptoethanol (C2H6OS) and 2,2′-azobisisobutyronitrile (AIBN) were purchased from 3A Chemicals Co. (China). Glacial acetic acid (AcOH), acetone, methanol (MeOH), dichloromethane (DCM), dimethyl ether (Et2O), isopropyl alcohol (IPA), 1,4-dioxane (C4H8O2), N,N-dimethylformamide (DMF) and other frequently-used chemical reagents were purchased from Guangzhou Chemical Reagent Company (Guangzhou, China). Human nasopharyngeal carcinoma cells (CNE-2) were provided from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Cell culture medium RPMI-1640, phosphate-buffered saline (PBS), fetal bovine serum (FBS), and 0.25% trypsin were obtained from Gibco BRL (Carlsbad, CA, USA). Hoechst 33342, 1,1′-dioctadecyl-3,3,3′,3′-tetramethyl indotricarbocyanine iodide (DiR), dichlorofluorescein (DCF), annexin V-FITC, propidium iodide (PI) and other fluorescent staining agents were supplied by Yeasen Biotechnology Co. Ltd (Shanghai, China).
7
Multifunctional Nanoparticle Synthesis and Characterization
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2.1 Chemicals and Characterization. 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), 2-methylimidazole (linker) and 1, 3-diphenylisobenzofuran (DPBF) were purchased from Damas-beta. Zinc nitrate hexahydrate (Zn(NO3)2•6H2O) and poly(acrylic acid, sodium salt) (PAAS) were from Strem and Sigma-Aldrich, respectively. Methyl alcohol, ethyl alcohol, isopropyl alcohol and absolute ethyl alcohol, were purchased from General-Reagent, China. (3aminopropyl) triethoxysilane (APTES) and Chlorin e6 (Ce6) were ordered from J&K and Frontier Scientific, respectively. Transmission electron microscopy (TEM) images were taken on a Tecnai G2 F20 S-TWIN. The UV-VIS absorption spectrum was determined with a UV-1900PC UV-visible spectrophotometer. Powder X-ray diffraction (XRD) patterns, Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS) were recorded using a BRUKER D8 instrument with Cu Kα radiation, Nicolet IS10 and Thermo escalab 250Xi, respectively. The L13152 Invitrogen TM Molecular Probes TM LIVE/DEAD® BacLight Bacterial Viability Kit was obtained from Thermo Fisher Scientific.
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