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Adipogenic induction medium

Manufactured by ScienCell
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Sourced in United States, China

Adipogenic induction medium is a cell culture medium designed to support the differentiation of cells, such as mesenchymal stem cells, into adipocytes (fat cells). The medium provides the necessary growth factors and nutrients to promote the conversion of precursor cells into mature adipocytes.

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Lab products found in correlation

Alkaline phosphatase, by Beyotime (1 mentions) Oil red o, by Solarbio (1 mentions) Alizarin red s, by Solarbio (1 mentions) Chondrogenic differentiation medium, by Procell (1 mentions)

2 protocols using adipogenic induction medium

1

Multilineage Differentiation of ADMSCs

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After passage 3 ADMSCs were grown to a confluence of 80% to 90%, the medium was replaced with osteoblast induction medium (ScienCell, USA) or adipogenic induction medium (ScienCell, USA). Alkaline phosphatase (Beyotime, China) staining was performed on the 9th day of induction, and Alizarin red S (Solarbio, China) staining was performed on the 21st day of induction to evaluate osteogenesis. Oil red O (Solarbio, China) staining was performed on the 14th day of induction to evaluate lipid droplet formation. Staining was observed and imaged under a microscope (Olympus Corporation, Japan).
Li C., An Y., Sun Y., Yang F., Xu Q, & Wang Z. (2022). Adipose Mesenchymal Stem Cell-Derived Exosomes Promote Wound Healing Through the WNT/β-catenin Signaling Pathway in Dermal Fibroblasts. Stem Cell Reviews and Reports, 18(6), 2059-2073.
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2

Adipogenic and Chondrogenic Differentiation of MMSCs

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MMSCs-M were cultured with adipogenic induction medium (ScienCell, China) or Chondrogenic differentiation medium (Procell, China) for 14 days. Oil Red O staining assay (VivaCell Bioscience) and Chondro-dye (VisaCell Biosciences) were applied to detect the ability of adipogenic and Chondrogenic differentiation following the instruction. Finally, the pictures were observed by the microscope.
Li S., Gao L., Zhang W., Yu Y., Zheng J., Liang X., Xin S., Ren W, & Zhi K. (2022). MiR-152-5p suppresses osteogenic differentiation of mandible mesenchymal stem cells by regulating ATG14-mediated autophagy. Stem Cell Research & Therapy, 13, 359.
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