The mouse thigh was split into 4 sections between hip and knee, fixed in 10% buffered formalin, and embedded in paraffin. Tissues were cut at a 4 µm thickness using a Leica RM 2255 rotary microtome and the cross-sections were stained with Hematoxylin and Eosin or Prussian blue stain to analyze tissue morphology and locate SPIONs, respectively. Tissue sections were visualized with a NanoZoomer Slider Scanner/Digital Pathology System (Hamamatsu).
Nanozoomer slider scanner digital pathology system
Manufactured by Hamamatsu Photonics
The NanoZoomer Slider Scanner/Digital Pathology System is a high-resolution digital slide scanner designed for scanning and digitizing microscope slides. It captures high-quality images of slide samples at various magnification levels, enabling digital analysis and archiving of pathological specimens.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using nanozoomer slider scanner digital pathology system
1
Mouse Thigh Tissue Analysis
2
Quantifying Necrosis in Histological Samples
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After EBs had been fixed in 10% buffered formalin for four hours and embedded in paraffin, they were sectioned into 3 µm thick sections. The sections were stained with Hematoxylin and Eosin (H&E), and photographed with a NanoZoomer Slider Scanner/Digital Pathology System (Hamamatsu). Areas filled with uniform eosinophilic cellular debris, with lost differential staining of nuclear and cellular details, were recognized as areas of necrosis. Fraction of necrotic cells in histological section was quantified by counting numbers of positively stained pixels using NIH ImageJ software.
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