Upright lsm 510 2 photon confocal microscope

Manufactured by Zeiss

The Upright LSM 510 2-Photon confocal microscope is a high-performance laboratory instrument designed for advanced imaging applications. It utilizes a 2-photon excitation method to capture detailed images of biological samples with improved depth penetration and reduced photodamage compared to conventional confocal microscopy. The core function of this microscope is to provide researchers with a powerful tool for visualizing and analyzing complex 3D structures within living cells and tissues.

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Lab products found in correlation

γh2ax antibody, by Merck Group (1 mentions) Oxiselect comet assay kit, by Cell Biolabs (1 mentions) Tsa biotin system, by PerkinElmer (1 mentions)

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LSM 510 (2630 citations) LSM 510 confocal microscope (1169 citations) LSM 510 microscope (264 citations) Zeiss 510 confocal microscope (104 citations) LSM 510 upright confocal microscope (19 citations) Confocal LSM 510 (10 citations) Upright LSM 510 2-photon microscope (2 citations) LSM 510 upright microscope (2 citations) 510 upright confocal microscope (2 citations) 510 Upright Microscope (2 citations)

2 protocols using upright lsm 510 2 photon confocal microscope

DNA Damage Quantification via Immunofluorescence and Comet Assay

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IF was performed using a γ-H2AX antibody (EMD Millipore; Billerica, MA) and a Cyclin A antibody (Abcam; Cambridge, MA) as described previously (25 (link)). For comet assay, an OxiSelect comet assay kit (Cell Biolabs; San Diego, CA) was used under a neutral condition following the manufacturer's protocol. Fluorescent images were captured using a Princeton Instruments cooled CCD digital camera from a Zeiss upright LSM 510 2-Photon confocal microscope. Olive tail moment was calculated using the formula tail DNA% × tail moment length.

Liu L., Zhou W., Cheng C.T., Ren X., Somlo G., Fong M.Y., Chin A.R., Li H., Yu Y., Xu Y., O'Connor S.T., O'Connor T.R., Ann D.K., Stark J.M, & Wang S.E. (2014). TGFβ Induces ‘BRCAness’ and Sensitivity to PARP Inhibition in Breast Cancer by Regulating DNA Repair Genes. Molecular cancer research : MCR, 12(11), 1597-1609.

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Immunohistochemistry Using Formaldehyde-Fixed Tissue

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The frozen section slides were fixed in 3.7% formaldehyde, blocked with 10% normal horse serum from the ABC kit (VECTASTAIN Elite PK-6200; Vector), and incubated overnight with primary antibody at 4°C. The biotinylated universal secondary antibody (ABC Kit) complex method and the TSA Biotin system (Perkin Elmer, catalog number: NEL744001KT) were used for detection. In the final step, the slides were counter-stained with DAPI and observed under a Zeiss Upright LSM510 2-Photon confocal microscope.

Wang J., Guo X., Lui M., Chu P.J., Yoo J., Chang M, & Yen Y. (2014). Identification of a Distinct Small Cell Population from Human Bone Marrow Reveals Its Multipotency In Vivo and In Vitro. PLoS ONE, 9(1), e85112.

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