Phenyl ether

Anhydrous iron (II) chloride (98%, Aldrich), anhydrous cobalt (II) chloride (99.7%, Alfa Aesar), sulfur powder (99.998%, Aldrich), chloroform (99.5%, Aldrich), anhydrous ethanol (99.5%, Aldrich), octadecylamine (90%, Acros), phenyl ether (99%, Acros) and argon gas (99.999%, Praxair) were used as received.

Iron(III) acetylacetonate (≥99%), absolute ethanol, n-hexane, toluene, acetonitrile, and C18 ziptips were purchased from Merck (Darmstadt, Germany). Standard proteins (fetuin, horseradish peroxidase (HRP)), phosphate buffer saline (PBS), acetic acid (AA), formic acid (FA), α-cyano-4-hydroxycinnamic acid (CHCA), ammonia (25%), 1,2-hexadecanediol (90%), (3-aminopropyl) trimethoxysilane (97%), sodium hydroxide (97%), tris(2-carboxyethyl) phosphine (≥98%), triethylammonium bicarbonate (TEABC), ninhydrin, and chloroform were obtained from Sigma Aldrich (St. Louis, MI, USA). Tetraethyl orthosilicate (≥98%), phenyl ether (99%), and oleylamine (80–90%) were purchased from Acros Organics (Fair Lawn, NJ, USA). N-dodecylphosphocholine was obtained from Anatrace (Maumee, OH USA). Trifluoroacetic acid (TFA) and ethyl acetate were purchased from Wako pure chemical industry (Osaka, Japan). Dithiothreitol (DTT) and iodoacetamide (IAM) were purchased from JT Baker (Phillipsburg, NJ, USA). Modified sequencing-grade trypsin was purchased from Promega (Madison, WI, USA). Protease inhibitor was purchased from Roche (Mannheim, Germany). SDB-XC Empore^TM disk membranes were purchased from 3M^TM (St. Paul, MN, USA).

Iron acetylacetonate (Fe(acac)₃), cobalt acetylacetonate (Co(acac)₂), phenyl ether, benzyl ether, oleic acid (OA), and absolute ethanol were obtained from (ACROS ORGANIC, Morris Plains, NJ, USA), Tungsten hexachloride (WCl₆) and 1-octadecene were purchased from (Alfa Aesar Inc., Haverhill, MA, USA), Oleylamine (OAm), hexane, ethylene glycol, 3-(3,4-dihydroxy)hydrocinnamic acid (DHCA), tetrahydrofuran (THF), NaOH, and phosphate buffered saline (PBS, concentration of PO₄²⁻ ions (0.0067 M) were acquired from Tokyo Chemical Industry (TCI Co, Tokyo, Japan), (Fisher chemicals, Pittsburgh, PA, USA), (J.T.Baker, Phillipsburg, NJ, USA), (Sigma Aldrich, St. Louis, MO, USA) (ECHO CHEMICAL CO., LTD., Taipei, Taiwan), (SHOWA Chemical, Tokyo, Japan), and HyClone^TM, Marlborough, MA, USA) respectively. The above chemicals were used without further purification. The 3-[4,5-dimethylthiazol-2yl]-2,5-diphenyl tetrazolium bromide (MTT) and Dulbecco’s Phosphate Buffered Saline (DPBS) were purchased from (Sigma Aldrich, St. Louis, MO, USA).