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Sfm4cho medium

Manufactured by GE Healthcare
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Sourced in United Kingdom, United States

SFM4CHO medium is a serum-free, chemically defined medium designed for the culture of Chinese Hamster Ovary (CHO) cells. It provides the necessary nutrients and growth factors to support the proliferation and viability of CHO cells in suspension culture.

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Lab products found in correlation

1 13c glucose, by Cambridge Isotopes (1 mentions) 1 2 13c glucose, by Cambridge Isotopes (1 mentions) 3 13c glucose, by Cambridge Isotopes (1 mentions) 1 6 13c glucose, by Cambridge Isotopes (1 mentions) 2 13c glucose, by Cambridge Isotopes (1 mentions) 4 5 6 13c glucose, by Cambridge Isotopes (1 mentions) 1 2 13c xylose, by Cambridge Isotopes (1 mentions) M9 minimal medium, by Merck Group (1 mentions) Dmem medium, by Corning (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions)

3 protocols using sfm4cho medium

1

Serum-free Suspension Adapted CHO-K1

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A null CHO-K1 cell line (ATCC, Manassas, VA, USA) was adapted to serum-free, suspension culture in 125 mL shake flasks containing 20–30 mL SFM4CHO medium (GE Healthcare Life Sciences, Little Chalfont, UK). Following adaptation, the cells were subjected to extended culture with routine passaging at 3–5 day intervals in a 37 °C cell culture incubator at 5% CO2 and 80% relative humidity.
Chiu J., Valente K.N., Levy N.E., Min L., Lenhoff A.M, & Lee K.H. (2016). Knockout of a difficult-to-remove CHO host cell protein, lipoprotein lipase, for improved polysorbate stability in monoclonal antibody formulations. Biotechnology and bioengineering, 114(5), 1006-1015.
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2

Isotopic Tracer Analysis of Glucose Metabolism

Cited 1 time
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Chemicals and M9 minimal medium were purchased from Sigma-Aldrich (St. Louis, MO). Isotopic tracers were purchased from Cambridge Isotope Laboratories (Tewksbury, MA): [1,6-13C]glucose (99.2 % 13C), [1,2-13C]glucose (99.7 %), [1-13C]glucose (99.5 %), [2-13C]glucose (99.5 %), [3-13C]glucose (99.5 %), [4,5,6-13C]glucose (99.5 %), and [1,2-13C]xylose (99.2 %). The isotopic enrichment of all tracers and the composition of tracer mixtures used in parallel labeling experiments were validated by GC-MS analysis as described in (Sandberg et al., 2016 (link)) and (Cordova and Antoniewicz, 2016 (link)). SFM4CHO medium (GE Healthcare Life Sciences SH3054901) and DMEM medium (Corning 17-207-CV, without glucose, glutamine, and sodium pyruvate) were purchased from Fisher Scientific (Pittsburgh, PA).
Long C.P., Au J., Gonzalez J.E, & Antoniewicz M.R. (2016). 13C metabolic flux analysis of microbial and mammalian systems is enhanced with GC-MS measurements of glycogen and RNA labeling. Metabolic engineering, 38, 65-72.
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3

CHO DG44 Cell Culture Protocol

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The CHO DG44 cell line expressing an aggregation-prone mAb was used as model system. The cells were seeded at 2–4 × 105 mL−1 in SFM4CHO medium (GE Healthcare, Chicago, IL, USA), incubated at 37 °C and 5% CO2, and passaged every 3–4 days. Glucose and l-glutamine (Life Technologies, Carlsbad, CA, USA) were added to the medium upon usage. All chemicals were ordered from Roth (Karlsruhe, Germany) if not stated otherwise.
Schwab K, & Hesse F. (2017). Estimating Extrinsic Dyes for Fluorometric Online Monitoring of Antibody Aggregation in CHO Fed-Batch Cultivations. Bioengineering, 4(3), 65.
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