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V. vulnificus is a bacterial strain maintained by the American Type Culture Collection (ATCC) for research purposes. It is a Gram-negative, halophilic, and potentially pathogenic bacterium. The core function of this strain is to serve as a reference culture for scientific investigations.

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Lab products found in correlation

V parahaemolyticus, by American Type Culture Collection (3 mentions) V alginolyticus, by American Type Culture Collection (2 mentions) S aureus, by American Type Culture Collection (1 mentions) E coli o157 h7, by American Type Culture Collection (1 mentions) L monocytogenes, by American Type Culture Collection (1 mentions) B cereus, by American Type Culture Collection (1 mentions) V cholerae, by American Type Culture Collection (1 mentions) C jejuni, by American Type Culture Collection (1 mentions) C perfringens, by American Type Culture Collection (1 mentions) Y enterocolitica, by American Type Culture Collection (1 mentions) Yeast extract, by HiMedia (1 mentions) Proteose peptone, by HiMedia (1 mentions)

3 protocols using v vulnificus

1

Microbial Strain-specific Capture Probe Design

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For the design of specific capture probes, 23 strains of 16 microbial species were used (Supporting information, Table S1): B. cereus (American Type Culture Collection (ATCC; Manassas, VA, USA) 11778, 13061, and 14579), C. jejuni (ATCC 33291), C. perfringens (ATCC 13124), E. coli (ATCC 25922), E. coli O157:H7 (ATCC 43894), L. monocytogenes (ATCC 15313), S. boydii (ATCC 8700 and 35966), S. dysenteriae (ATCC 13313), S. sonnei (ATCC 9290, 25931, and 29930), S. aureus (ATCC 6538), S. enterica subsp. enterica serotype Choleraesuis (ATCC 7001, 10708, and 13312), S. enterica subsp. enterica serotype Enteritidis (ATCC 31194), V. cholerae (ATCC 14035), V. parahaemolyticus (ATCC 17802), V. vulnificus (ATCC 27562), and Y. enterocolitica (ATCC 23751).
Shin H.H., Hwang B.H, & Cha H.J. (2016). Multiplex 16S rRNA‐derived geno‐biochip for detection of 16 bacterial pathogens from contaminated foods. Biotechnology Journal, 11(11), 1405-1414.
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2

Vibrio Strain Collection Protocols

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Five Vibrio strains were obtained from the collection of the Institute Pasteur (CIP): V. cholerae CIP 62.13; V. cholerae CIP 55.91 (O1 classical); V. cholerae CIP 106855 (O1 El Tor); V. cholerae CIP 104151 (O139); and V. natriegens CIP 103193.
The National Center for Disease Control (NCDC) in Tbilisi, Georgia kindly provided two strains of V. cholerae: V. cholerae N145/P-1 (O1 classical) and V. cholerae N890/M-878 (O1 El Tor).
The following reference strains were obtained from the American Type Culture Collection (ATCC): V. vulnificus ATCC 27562; V. parahaemolyticus ATCC 17802; V. metschnikovii ATCC 700040; V. fluvialis ATCC 33809; V. furnissii ATCC 35016; V. alginolyticus ATCC 17749 serotype XII; Photobacterium damselae subsp. damselae ATCC 33539; Grimontia (Vibrio) hollisae ATCC 33564; V. cincinnatiensis ATCC 35912; and V. harveyi ATCC 14126.
Kokashvili T., Whitehouse C.A., Tskhvediani A., Grim C.J., Elbakidze T., Mitaishvili N., Janelidze N., Jaiani E., Haley B.J., Lashkhi N., Huq A., Colwell R.R, & Tediashvili M. (2015). Occurrence and Diversity of Clinically Important Vibrio Species in the Aquatic Environment of Georgia. Frontiers in Public Health, 3, 232.
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3

Vibrio Strains Cultivation and Preservation

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Vibrio strains were bought from the Bioresource Collection and Research Center, Taiwan, including V. alginolyticus ATCC 17749, V. carchariae ATCC 35084, V. damsela ATCC 33536, V. harveyi ATCC 14126, V. parahaemolyticus ATCC 17802, V. pelagius ATCC 25916, and V. vulnificus BCRC15431. The Vibrio strains were maintained in Bacto Brain Heart Infusion medium (BHI; Becton-Dickinson Co, USA) supplemented with 3% NaCl (Panreac Quimica, France). For long-term preservation, bacteria were frozen at −80°C in BHI supplemented with 1% NaCl and 25% glycerol (Nihon Shiyaku, Japan). The strains were streaked onto modified seawater yeast extract (rich MSWYE) agar plates consisting of 23.4 g NaCl, 6.98 g MgSO4_7 H2O, and 0.75 g KCl in 1000 ml distilled water [18 (link)]. The pH was adjusted to 7.6 with 1 N NaOH, followed by the addition of 5.0 g of proteose peptone (HIMEDIA Lab, India), 3.0 g of yeast extract (HIMEDIA Lab, India), and 20.0 g of agar per liter.
Liu W., Lin Y.R., Lu M.W., Sung P.J., Wang W.H, & Lin C.S. (2014). Genome sequences characterizing five mutations in RNA polymerase and major capsid of phages ϕA318 and ϕAs51 of Vibrio alginolyticus with different burst efficiencies. BMC Genomics, 15(1), 505.
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