Zymutest pai 1 activity

Several coagulation and fibrinolytic parameters, not belonging to the classical thrombophilia screening, were measured in 10 family members with plasma available (3 cases and 7 controls). The 3 cases were on warfarin whereas none of the controls were on anticoagulant treatment at the time of the sampling.
Activated partial thrombin time (aPTT) and prothrombin time (PT), fibrinogen, factor II, factor V and factor X were measured in plasma with automated coagulometers (STA-Revolution, Diagnostica Stago, Asnières, France) using established commercial assays. Free tissue factor pathway inhibitor (f-TFPI) plasma levels were measured with the Asserachrom Free TFPI enzyme immunoassay from Diagnostica Stago (Asnieres, France) and plasma PAI-1 activity with the Zymutest PAI-1 activity from Hyphen Biomed (Neuville-sur-Oise, France). Urokinase (uPA) plasma levels were measured with the uPA ELISA kit (Abcam, Cambrige, UK). The euglobulin clot lysis time (ECLT) assay has been performed as previously described [29 (link)].

Platelet poor plasma was obtained by centrifugation of citrated blood at 2500 g for 20 min at room temperature. Aliquots of citrated plasma, 1 ml each, were stored, shortly after collection, at -80°C until PAI-1 antigen, PAI-1 activity, TAFI assay were performed.
D-dimer concentrations were measured in citrated plasma by the d-dimer HS kit on ACL TOP analyzer (Instrumentation Laboratory, IL, USA). PAI-1 antigen was analyzed in duplicate by the ELISA kit ZYMUTEST PAI-1 Antigen (HYPHEN Biomed France). PAI-1 activity was measured in duplicate by the ELISA kit ZYMUTEST PAI-1 Activity (HYPHEN Biomed France). TAFI antigen was analyzed in duplicate by the IMUCLONE TAFI ELISA (Sekisui Diagnostics USA).