Mouse anti-rodent proinsulin (CCI-17) antibody was from Novus Biologicals (catalog no.: NB100-73013). Mouse anti-beta actin antibody was from Proteintech (catalog no.: 66009-1-Ig). Mouse anti-tubulin antibody was from Sigma (catalog no.: T5168). Rabbit anti-phosphorylation eIF2α antibody was from Cell Signaling Technology (catalog no.: 9721S). Rabbit anti-GM130 antibody was from Abcam (catalog no.: ab52649). DTT, MG-132, CHX, and N-ethylmaleimide were from Sigma. GCN2 inhibitor was from MedChemExpress (catalog no.: HY-112654). CB-5083 was from APExBIO (catalog no.: B6032). Met/Cys-deficient Dulbecco's modified Eagle's medium (DMEM) and all other tissue culture reagents were from Invitrogen. 35S-amino acid mixture was from PerkinElmer. Protein A-Agarose was from Repligen.
Gcn2 inhibitor
Manufactured by MedChemExpress
GCN2 inhibitor is a chemical compound that specifically inhibits the activity of the GCN2 (General Control Nonderepressible 2) protein. GCN2 is a serine/threonine-protein kinase that plays a role in the cellular response to amino acid deprivation. The GCN2 inhibitor can be used as a research tool to study the biological functions of the GCN2 protein.
Automatically generated - may contain errors
Lab products found in correlation
Cycloheximide (chx), by Merck Group (2 mentions)
Mg132, by Merck Group (2 mentions)
Dithiothreitol (dtt), by Merck Group (2 mentions)
Mouse anti tubulin antibody, by Merck Group (1 mentions)
Cb 5083, by Apexbio (1 mentions)
Mouse anti beta actin antibody, by Proteintech (1 mentions)
Rabbit anti gm130 antibody, by Abcam (1 mentions)
Met cys deficient dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
35s amino acid mixture, by PerkinElmer (1 mentions)
Protein a agarose, by Repligen (1 mentions)
N ethylmaleimide, by Merck Group (1 mentions)
Rabbit anti phospho eif2α ser51, by Cell Signaling Technology (1 mentions)
Rabbit mab anti hsp90, by Cell Signaling Technology (1 mentions)
Mouse anti α tubulin mab, by Merck Group (1 mentions)
Rneasy plus mini kit, by Qiagen (1 mentions)
2 protocols using gcn2 inhibitor
1
Antibody and Reagent Identification for Cellular Assays
2
Antibody Characterization for Insulin Detection
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Antibodies included guinea pig anti-insulin (Covance, RRID:AB_10013624 ), mouse mAb anti-rodent proinsulin (Novus Biologicals, RRID:AB_1107982 ), mouse mAb anti-human proinsulin B-C junction sequence KTRREAEDLQ (Abmart, RRID:AB_2921300 ), a custom-made rabbit polyclonal antibody against mouse C-peptide-2 (C-PQVAQLELGGGPGAGDLQT, Abmart, RRID:AB_2921302 ), mouse mAb anti-α-tubulin (Sigma-Aldrich, RRID:AB_477579 ), mouse mAb anti-beta actin (Proteintech, RRID:AB_2919667 ), rabbit mAb anti-HSP90 (Cell Signaling, RRID:AB_2233307), and rabbit anti-phospho-eIF2α (Ser51) (Cell Signaling, RRID:AB_330951 ). All tissue culture reagents were from Invitrogen. Dithiothreitol, MG132, and CHX were from Sigma. GCN2 inhibitor was from MedChemExpress (HY-112654). Mycolactone was isolated from Mycobacterium ulcerans, as described (49 ). RNeasy Plus Mini Kit was from Qiagen (cat. #74134). Anti-flag magnetic agarose beads were from Fisher Scientific.
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