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Ceacam6

Manufactured by Santa Cruz Biotechnology
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CEACAM6 is a protein that is involved in cell adhesion and is expressed on the surface of various cell types. It belongs to the carcinoembryonic antigen (CEA) family of proteins. CEACAM6 plays a role in cell-cell interactions and may be involved in processes such as immune regulation and cancer progression.

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Lab products found in correlation

Aldob, by Proteintech (1 mentions) Phospho pdk1, by Cell Signaling Technology (1 mentions) Puromycin, by Thermo Fisher Scientific (1 mentions) Blasticidin, by Thermo Fisher Scientific (1 mentions) Lenti x 293t cells, by Takara Bio (1 mentions) C fos, by Cell Signaling Technology (1 mentions) P src y416, by Cell Signaling Technology (1 mentions) Celltiter glo, by Promega (1 mentions) Brms1, by Abcam (1 mentions) V5 epitope, by Abcam (1 mentions) P jun s63, by Cell Signaling Technology (1 mentions) Ramp1, by Thermo Fisher Scientific (1 mentions) Src inhibitor saracatinib, by Selleck Chemicals (1 mentions) C jun, by Cell Signaling Technology (1 mentions) β actin, by Merck Group (1 mentions) Ceacam5, by Abcam (1 mentions) Phosphor fak, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Mouse anti-CEACAM6 monoclonal antibody (9A6, (2 citations)

4 protocols using ceacam6

1

Western Blot Analysis of Metabolic Enzymes

Cited 2 times
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Western blots were conducted following previously described methods [30 (link)]. The rabbit polyclonal antibodies against ALDOB (Proteintech, Rosemont, IL, USA, Cat. 18065-1-AP, diluted 1:5000), LDHA (Proteintech, Cat. 21799-1-AP, diluted 1:1000), and LDHB (Proteintech, Cat. 14824-1-AP, diluted 1:5000), along with the rabbit monoclonal antibody against ACTB (Sigma-Aldrich, St. Louis, MO, USA, Cat. A5441, diluted 1:10000), PDK1 (Cell Signaling Technology, Danvers, MA, USA, Cat. #5662, diluted 1:1000), phospho-PDK1 (Cell Signaling Technology, Cat. #3438, diluted 1:1000), and lactyl-lysine (Cat. PTM-1401RM, PTM BIO, Chicago, IL, USA), and the mouse monoclonal antibodies against pan-CEA (Santa Cruz Biotechnology, Dallas, TX, USA, Cat. Sc-48364, diluted 1:100), and CEACAM6 (Santa Cruz Biotechnology, Cat. sc59899, diluted 1:100) were used for Western blot analysis. Lysate preparation and immunoprecipitation were performed according to previously established methods [31 (link)]. In each reaction, 1 μg of the mouse monoclonal antibody against CEACAM6 (Santa Cruz Biotechnology, Cat. sc59899) was employed. After thorough washing, the immunoprecipitates were utilized for subsequent western blot analysis.
Chu Y.D., Cheng L.C., Lim S.N., Lai M.W., Yeh C.T, & Lin W.R. (2023). Aldolase B-driven lactagenesis and CEACAM6 activation promote cell renewal and chemoresistance in colorectal cancer through the Warburg effect. Cell Death & Disease, 14(10), 660.
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2

LUAD Tissue and Cell Line Protocol

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Fresh resected human LUAD and matched adjacent noncancerous tissue were obtained after informed consent and approval by the institutional review board (16-1514, 16-107, and 12-245) at Memorial Sloan Kettering Cancer Center. Human LUAD cell lines, human lung fibroblast cells and a normal bronchial epithelial cell line (NL20) were obtained from American Type Culture Collection; Lenti-X 293T cells were purchased from Takara Bio. All cells were tested for mycoplasma. The primary antibodies used were BRMS1 (Abcam, ab134968), c-fos (Cell Signaling, #2250), c-Jun (Cell Signaling, #9165), V5-epitope (Abcam, ab15828), CEACAM6 (Santa Cruz, SC-59899), Src (Cell Signaling, #2109), p-Src Y416 (Cell Signaling, #59548), p-fos S32 (Cell Signaling, #5248), actin (Santa Cruz, sc-8432), p-Jun S63 (Cell Signaling, #2361), RAMP1 (Thermo Fisher, 10327-1-AP), and F5 (Thermo Fisher, PA5-103046). The reagents used—c-fos inhibitor T5224 (S8966) and Src inhibitor Saracatinib (AZD0530, S1006)—were purchased from Selleck Chemicals. Collagen Type IV (C6745), Collagen Type I solution (C3867), and TCN (T7760) were purchased from Millipore Sigma; puromycin (A1113803) and blasticidin (A1113903) were purchased from Thermo Fisher; CellTiter-Glo was obtained from Promega (G9241); and poly-HEMA solution was purchased from Sigma-Aldrich (P3932).
Liu Y., Chudgar N., Mastrogiacomo B., He D., Lankadasari M.B., Bapat S., Jones G.D., Sanchez-Vega F., Tan K.S., Schultz N., Mukherjee S., Offit K., Bao Y., Bott M.J., Rekhtman N., Adusumilli P.S., Li B.T., Mayo M.W, & Jones D.R. (2022). A germline SNP in BRMS1 predisposes patients with lung adenocarcinoma to metastasis and can be ameliorated by targeting c-fos. Science translational medicine, 14(665), eabo1050.
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3

Antibody Procurement for Cell Signaling

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Antibodies against ZO-1 were purchased from Invitrogen; CEACAM5 from Abcam (Cambridge, UK); CEACAM6 from Santa Cruz Biotechnology (Dallas, TX); β-actin from Sigma; phosphor-FAK from Invitrogen; FAK from BD Bioscience (San Jose, CA); and integrin β4 from Novous Biological (Littleton, CO).
Sato Y., Tateno H., Adachi J., Okuyama H., Endo H., Tomonaga T, & Inoue M. (2016). Generation of a monoclonal antibody recognizing the CEACAM glycan structure and inhibiting adhesion using cancer tissue-originated spheroid as an antigen. Scientific Reports, 6, 24823.
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4

Immunohistochemical Quantification of ALDOB and CEACAM6

Cited 1 time
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IHC was conducted following previously described methods [32 (link)]. A rabbit monoclonal antibody against ALDOB (Proteintech, Rosemont, IL, USA, Cat. 14824-1-AP) and CEACAM6 (Santa Cruz Biotechnology, Cat. sc59899) were used for staining at dilutions of 1:200 and 1:50, respectively. The intensity of the staining signal was quantified using Image J (National Institutes of Health, Bethesda, MD, USA, Fiji edition) and subsequently analyzed.
Chu Y.D., Cheng L.C., Lim S.N., Lai M.W., Yeh C.T, & Lin W.R. (2023). Aldolase B-driven lactagenesis and CEACAM6 activation promote cell renewal and chemoresistance in colorectal cancer through the Warburg effect. Cell Death & Disease, 14(10), 660.
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