The spared nerve injury (SNI) model of neuropathic pain was performed under Avertine (2,2,2-tribromoethanol, Sigma-Aldrich) general anesthesia, as previously described (7 (link), 41 (link)). Briefly, skin and muscle incisions were made on the left hind leg at mid-thigh level, revealing the sciatic nerve and its three branches. The common peroneal and sural nerves were carefully ligated with 6.0 silk suture (Ethicon, Johnson & Johnson Intl.), transected, and a 1–2mm sections of each of these nerves were removed. The tibial nerve was left intact. Skin was then closed and stitched with silk 4.0 sutures (Ethicon, Johnson & Johnson Intl). Sham operated mice were exposed to the same procedure but all nerves were left intact.
Avertine
Manufactured by Merck Group
Avertine is a laboratory equipment product developed by Merck Group. It is designed for precise and efficient liquid handling tasks in various scientific and research applications.
Automatically generated - may contain errors
5 protocols using avertine
1
Spared Nerve Injury Model of Neuropathic Pain
2
Genistein Modulation of Gliadin-Induced Celiac Disease
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
BALB/c mice (background BALB/cAnNCrl) [35 (link)] were purchased from Charles River (Varese, Italy). Three-generation gluten-free diet (Mucedola srl, Milan), male and female, were challenged with gliadin for 4 weeks [34 (link)–37 (link)]. To assess the effects of Genistein or VX-770 into a controlled environment, mice were challenged via gavage for 4 weeks with i) vehicle alone or ii) gliadin (Sigma-Aldich, G3375) (5 mg/daily for one week and then 5 mg/daily thrice a week for 3 weeks) [12 (link),34 (link)–37 (link)] in the presence or absence of intraperitoneal genistein (25mg\kg in 100µl DMSO, Sigma-Aldrich) administered 15 minutes prior each gliadin challenge (n=10 mice per group of treatment).
At the end of the last daily treatment, mice were anesthetized with Avertine (tribromoethanol, 250 mg/kg, Sigma Aldrich, T48402) and then killed; the intestines were collected for CFTR function analysis or stored for all described techniques.
These studies and procedures were approved by the local Ethics Committee for Animal Welfare (IACUC No849) and conformed to the European Community regulations for animal use in research (2010/63 UE).
At the end of the last daily treatment, mice were anesthetized with Avertine (tribromoethanol, 250 mg/kg, Sigma Aldrich, T48402) and then killed; the intestines were collected for CFTR function analysis or stored for all described techniques.
These studies and procedures were approved by the local Ethics Committee for Animal Welfare (IACUC No849) and conformed to the European Community regulations for animal use in research (2010/63 UE).
3
Perfusion-Based Brain Histology
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice were anesthetized by intraperitoneal injection of 10 mg/ml avertine (Sigma) and transcardially perfused with 4% paraformaldehyde in 0.1 M phosphate buffer (pH 7.4). Dissected brains were further fixed for 48 h at 4 °C before paraffin embedding and sectioning. Four micrometers sections were stained with hematoxylin and eosin and examined under the optical microscope.
4
Sciatic Nerve Conduction Velocity Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Neurophysiology was performed as described [38 (link)]. The dy2J mice were analyzed before (45 days of age) and after (85 days of age) MAB treatment (see scheme in Fig. 5a ). Mice were anesthetized with tribromoethanole (Avertine; Sigma) and placed under a heating lamp to avoid hypothermia. Sciatic nerve conduction velocities (NCV) were obtained by stimulating the nerve with steel monopolar needle electrodes. A pair of stimulating electrodes was inserted subcutaneously near the nerve at the ankle, and then they were moved to the sciatic notch to obtain two distinct sites of stimulation, proximal and distal along the nerve, in order to obtain the measurement of NCV between them. The muscular response to the electrical nerve stimulation (compound motor action potentials (cMAP)) was recorded with a pair of needle electrodes; the active electrode was inserted in muscles in the middle of the paw, while the reference was placed in the skin between the first and second digit. The cMAP peak-to-peak amplitude was considered for analysis. The examiner was blind as respect to the scheme of treatment.
5
Investigating Vitamin D Supplementation in High-Fat Diet-Induced Metabolic Alterations
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Juvenile C57BL/6 mice (5weeks; Charles River, Calco, LC) were liberally fed with either a standard diet (STD) or high fat diet (HFD; 60% energy from fat, Mucedola S.r.l, Settimo Milanese, MI, Italy) supplemented with 1000 IU vitamin D3 (10,000 IU/mL; 100 u l/mouse; Abiogen Pharma SpA, Pisa, PI, Italy), or vehicle (STD, n = 7; STDVD, n = 7; HFD, n = 5; HFDVD, n = 5) via gavage three times a week (wk) for up to 6 wks. The dosage of vitamin D3 (VD3) and intermittent administration are based on previous published studies, with a minimal dosage selected for the present investigation [35 (link)]. Baseline weights (gm) as well as weight gain (gm) and glucose measurements (mg/dl; caudal vein puncture with Breeze 2 measurements; Bayer, Leverkusen, Germany), were taken in each mouse each week up to the time of sacrifice. A second “long term” group of mice (STD; n = 5; STDVD; n = 5: HFD; n = 5: HFDVD; n = 5) performed the same protocol for up to 22 weeks. At the completion of the protocol, the mice were anesthetized with Avertine (tribromoethanol, 250 mg/kg, Sigma) and sacrificed, with plasma and liver tissue samples collected, snap frozen and stored at −80 °C. All the procedures in mice were approved by the local Ethics Committee for Animal Welfare (IACUC No. 583; IRCCS Oespedale San Raffaelle) and were carried out in compliance with the European and National regulations.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!