Tau12

Manufactured by BioLegend

Sourced in United States

Tau12 is a monoclonal antibody that recognizes Tau protein. Tau protein is a microtubule-associated protein that plays a role in the stabilization of microtubules. The Tau12 antibody can be used for the detection and analysis of Tau protein in various applications.

Automatically generated - may contain errors

Lab products found in correlation

Paramagnetic beads, by Quanterix (3 mentions) Mn1050, by Thermo Fisher Scientific (2 mentions) To8 50fn, by Sino Biological (1 mentions) Simoa hd x analyzer, by Quanterix (1 mentions) Simoa assay, by Quanterix (1 mentions) Simoa nf light assay, by Quanterix (1 mentions) Hd x analyser, by Quanterix (1 mentions) Simoa hd x, by Quanterix (1 mentions) Cf633, by Merck Group (1 mentions) Nab228, by Merck Group (1 mentions) Cf488a, by Merck Group (1 mentions)

6 protocols using tau12

Quantification of Alzheimer's Biomarkers

Check if the same lab product or an alternative is used in the 5 most similar protocols

All biomarkers were measured on the Simoa HD-X platform. CSF and BD-tau was measured according to Gonzalez-Ortiz et al., using TauJ5.H3(Bioventix) as capture antibody and Tau12 (BioLegend, #SIG-39416) as partner antibody^{23 ,25}. Plasma p-tau181 was measured either with a commercial method from Quanterix Inc. (pTau-181 V2 Advantage Kit #103714;) or according to the Karikari et al., method^{27 (link)} using an anti p-tau181 Tau antibody (Thermo Fisher, catalog number: MN1050) as capture and Tau12 (BioLegend, #SIG-39416) as partner antibody. Plasma T-tau and NfL were measured using Quanterix commercial kits (#101552 and #103670). A dilution factor of four was used for the serum and plasma samples, while a dilution factor of thirty was used for measurements in CSF.

Gonzalez-Ortiz F., Kirsebom B.E., Contador J., Tanley J.E., Selnes P., Gísladóttir B., Pålhaugen L., Suhr Hemminghyth M., Jarholm J., Skogseth R., Bråthen G., Grøndtvedt G., Bjørnerud A., Tecelao S., Waterloo K., Aarsland D., Fernández-Lebrero A., García-Escobar G., Navalpotro-Gómez I., Turton M., Hesthamar A., Kac P.R., Nilsson J., Luchsinger J., Hayden K.M., Harrison P., Puig-Pijoan A., Zetterberg H., Hughes T.M., Suárez-Calvet M., Karikari T.K., Fladby T, & Blennow K. (2024). Plasma brain-derived tau is an amyloid-associated neurodegeneration biomarker in Alzheimer’s disease. Nature Communications, 15, 2908.

+ Open protocol

+ Expand

Phosphorylated Tau Protein Detection Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

A rabbit polyclonal antibody specific for p‐tau217 (#44‐744, Invitrogen) was used as capture, conjugated to paramagnetic beads (#103207, Quanterix). The mouse monoclonal antibody Tau12 (#806502, BioLegend) raised against the N‐terminal epitope 6‐18aa was used for detection.³⁶Antibody specificity was independently validated.³⁷ The assay calibrator was phosphorylated recombinant full‐length tau‐441 (#TO8‐50FN, SignalChem). Calibrators and specimens were diluted with assay diluent (Tau 2.0 buffer; #101556, Quanterix). Analytical validation and assay measurement protocol are described in supporting information.
The N‐p‐tau181 assay, validated for blood,¹² was further validated for CSF (supporting information).

Karikari T.K., Emeršič A., Vrillon A., Lantero‐Rodriguez J., Ashton N.J., Kramberger M.G., Dumurgier J., Hourregue C., Čučnik S., Brinkmalm G., Rot U., Zetterberg H., Paquet C, & Blennow K. (2020). Head‐to‐head comparison of clinical performance of CSF phospho‐tau T181 and T217 biomarkers for Alzheimer's disease diagnosis. Alzheimer's & Dementia, 17(5), 755-767.

+ Open protocol

+ Expand

Quantitative Protein Biomarkers Measurement

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

NfL concentrations were measured using the NF-light™ kit on a Single molecule array (Simoa) HD-X Analyzer (Quanterix, Billerica, MA, USA), following the recommendations by the manufacturer. Plasma p-tau181 levels were measured using an in house Simoa assay as previously described [10 (link)]. Briefly, an AT270 mouse monoclonal antibody (MN1050; Invitrogen, Waltham, MA, USA) was coupled to paramagnetic beads (103,207; Quanterix) and used for capture. As the detector, we used the anti-tau mouse monoclonal antibody Tau12 (806,502; BioLegend, San Diego, CA, USA), conjugated to biotin (A3959; Thermo Fisher Scientific, Waltham, MA, USA), while GSK-3β phosphorylated full-length recombinant tau441 (TO8–50FN; SignalChem, Vancouver, BC, Canada) was used as calibrator. Fluorescent signals were converted to average enzyme per bead numbers as described [19 (link)], and specimen concentrations extrapolated from four-parametric logistic curves generated with known calibrator concentrations.

Clark C., Lewczuk P., Kornhuber J., Richiardi J., Maréchal B., Karikari T.K., Blennow K., Zetterberg H, & Popp J. (2021). Plasma neurofilament light and phosphorylated tau 181 as biomarkers of Alzheimer’s disease pathology and clinical disease progression. Alzheimer's Research & Therapy, 13, 65.

+ Open protocol

+ Expand

Quantitative Plasma Biomarker Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

Plasma was collected by venipuncture, processed, and stored in aliquots at − 80 °C according to the standardised procedures. Plasma NfL concentration was measured using a commercial single-molecule array (Simoa) NF-Light® assay (Quanterix, Billerica, MA) according to the manufacturer’s instructions [25 ]. Plasma p-Tau₁₈₁ concentration was measured using an in-house Simoa assay developed at the University of Gothenburg [8 (link)]. In brief, the capture antibody (AT270, Invitrogen) which is specific for the threonine-181 phosphorylation site [26 (link)] was coupled to paramagnetic beads whilst the detector antibody (Tau12, BioLegend) was raised against the N-terminal epitope amino acid 6-QEFEVMEDHAGT-18 on human tau protein. Detailed analytical procedures and assay validation have been previously described [8 (link)]. Plasma GFAP, Aβ_1–42, and Aβ_1–40 concentrations were measured using commercial Simoa assays (Quanterix, Billerica, MA). All measurements were carried out using an HD-X analyser (Quanterix, Boston, MA) in one round of experiments, using one batch of reagents with operators blinded to clinical information.

Benussi A., Cantoni V., Rivolta J., Archetti S., Micheli A., Ashton N., Zetterberg H., Blennow K, & Borroni B. (2022). Classification accuracy of blood-based and neurophysiological markers in the differential diagnosis of Alzheimer’s disease and frontotemporal lobar degeneration. Alzheimer's Research & Therapy, 14, 155.

+ Open protocol

+ Expand

Plasma p-tau181 Measurement Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Blood samples were collected and processed according to the ADNI protocol (Kang et al., 2015 (link)). Plasma p-tau181 concentrations were measured at the Clinical Neurochemistry Laboratory, University of Gothenburg (Mölndal, Sweden) using an assay developed in-house on a Simoa HD-X (Quanterix) instrument, as described previously in detail (Karikari et al., 2020 (link)). In brief, the AT270 mouse monoclonal antibody (MN1050; Invitrogen) specific for the threonine-181 phosphorylation site, coupled to paramagnetic beads (103 207; Quanterix) was used for capture and the anti-tau mouse monoclonal antibody Tau12 (806 502; BioLegend), which binds the N-terminal epitope 6-QEFEVMEDHAGT-18 on human tau protein, for detection. All of the available samples were analysed in a single batch. We identified four participants (0.4%) with outlier values of plasma p-tau181 levels that were discarded from subsequent analyses (Supplementary Fig. 1). Longitudinal blood sampling was performed approximately every year, over a median follow-up time of 2.9 years in 938 subjects.

Moscoso A., Grothe M.J., Ashton N.J., Karikari T.K., Rodriguez J.L., Snellman A., Suárez-Calvet M., Zetterberg H., Blennow K, & Schöll M. (2020). Time course of phosphorylated-tau181 in blood across the Alzheimer’s disease spectrum. Brain, 144(1), 325-339.

+ Open protocol

+ Expand

Oligomer Detection with Fluorescent Antibodies

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

To detect oligomers in samples, we labeled the antibodies Nab228 (Sigma‐Aldrich) and Tau12 (BioLegend, San Diego, CA, USA) with the fluorescent dyes CF633 (Sigma‐Aldrich) and CF488A (Sigma‐Aldrich) according to manufacturer's protocol. The principle of reaction, the purification, and the determination of concentration and degree of labeling were previously described.
¹⁹ (link)

Blömeke L., Rehn F., Kraemer‐Schulien V., Kutzsche J., Pils M., Bujnicki T., Lewczuk P., Kornhuber J., Freiesleben S.D., Schneider L., Preis L., Priller J., Spruth E.J., Altenstein S., Lohse A., Schneider A., Fliessbach K., Wiltfang J., Hansen N., Rostamzadeh A., Düzel E., Glanz W., Incesoy E.I., Butryn M., Buerger K., Janowitz D., Ewers M., Perneczky R., Rauchmann B., Teipel S., Kilimann I., Goerss D., Laske C., Munk M.H., Sanzenbacher C., Spottke A., Roy‐Kluth N., Heneka M.T., Brosseron F., Wagner M., Wolfsgruber S., Kleineidam L., Stark M., Schmid M., Jessen F., Bannach O., Willbold D, & Peters O. (2024). Aβ oligomers peak in early stages of Alzheimer's disease preceding tau pathology. Alzheimer's & Dementia : Diagnosis, Assessment & Disease Monitoring, 16(2), e12589.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!