Thinprep slide

Manufactured by Hologic

Sourced in United States

The ThinPrep slides are a type of laboratory equipment used for the preparation and analysis of cell samples. The slides provide a consistent and uniform presentation of cells, facilitating accurate examination and interpretation by medical professionals.

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Lab products found in correlation

Thinprep processor, by Hologic (1 mentions) Benchmark ultra system, by Roche (1 mentions) Aquatex, by Merck Group (1 mentions) Zen lite software, by Zeiss (1 mentions) Prolong gold antifade reagent, by Thermo Fisher Scientific (1 mentions) Preservcyt solution, by Hologic (1 mentions) Thinprep 2000, by Hologic (1 mentions) Ht1197, by American Type Culture Collection (1 mentions) Preservcyt, by Hologic (1 mentions) Cytorich red solution, by Thermo Fisher Scientific (1 mentions)

8 protocols using thinprep slide

Cervical Cytology Dual-Staining Evaluation

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Slides were prepared on a ThinPrep processor (T5000, Hologic Inc) using special ThinPrep slides (Hologic, Inc) and stained using CINtec PLUS test kits within 48 hours and processed on BenchMark ULTRA system (Roche Diagnostics) per manufacturer’s instructions.
The CINtec PLUS slides were initially evaluated independently by one of two experienced cytotechnologists who were trained to read these slides. Smears were determined to be positive if at least one cervical epithelial cell showed both a brownish cytoplasmic immunostaining for p16 and a red nuclear immunostaining for Ki-67 regardless of cellular morphology. If the dual staining was not observed, the smear was considered negative. Smears were deemed unsatisfactory if they did not contain an adequate number of cells (

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4 cells per field with a minimum of 10 fields with a 40x objective). All slides were independently reviewed by a study pathologist trained to read CINtec PLUS slides, and the results recorded using the same criteria. Discrepant slides were either internally reviewed by another reader and reconciled or adjudicated independently by an external expert.

Gilbert L., Ratnam S., Jang D., Alaghehbandan R., Schell M., Needle R., Ecobichon-Morris A., Wadhawan A., Costescu D., Elit L., Wang P., Zahariadis G, & Chernesky M. (2022). Comparison of CINtec PLUS cytology and cobas HPV test for triaging Canadian patients with LSIL cytology referred to colposcopy: A two-year prospective study. Cancer Biomarkers, 34(3), 347-358.

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Cytological Characteristics of Pancreatoblastoma

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After approval by the institutional review boards of the participating institutions (all primarily focused on adult pathology), a search of the archival pathology files was conducted, which yielded 12 fine-needle aspiration biopsies (FNABs) from 11 patients. Rapid on-site evaluation was performed on all cases that were deemed adequate at the time of evaluation. The cytology material, including smears, ThinPrep slides (Hologic Inc), and cell blocks, as well as immunostains, where available, were reviewed for the presence or absence of cytologic characteristics of PBL that were previously described in the literature, including hypercellularity, clusters, papillae, nuclear grooves, nuclear molding, pleomorphism, mitotic figures, necrosis, squamoid morules, stromal fragments, plasmacytoid tumor cells, tumor cells with high nuclear-to-cytoplasmic ratio, fine chromatin, and small size (similar to that of red blood cells [RBCs]). For the purpose of this study, these small tumor cells with high nuclear-to-cytoplasmic ratios and fine chromatin were characterized as blast-like pancreatoblastoma cells. The presence of cytoplasmic microvesicles and red granules was also noted.
In addition, 10 patients had histologic material (resection (n = 10) plus biopsy (n = 1)) which were also reviewed with confirmation of the diagnosis. The patients’ clinicopathologic information was also collated.

Reid M.D., Bhattarai S., Graham R.P., Pehlivanoglu B., Sigel C.S., Shi J., Saqi A., Shirazi M., Xue Y., Basturk O, & Adsay V. (2019). Pancreatoblastoma: Cytologic and Histologic Analysis of 12 Adult Cases Reveals Helpful Criteria in Their Diagnosis and Distinction From Common Mimics. Cancer cytopathology, 127(11), 708-719.

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Immunohistochemical Detection of HPV L1 Protein

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ThinPrep slides (Hologic, Bedford, MA, USA) were immunochemically stained as part of a Cytoactiv^® HPV L1 screening set (Cytoimmun Diagnostics, Pirmasens, Germany) to detect L1 capsid protein of all known HPV types, as previously described [8 (link)9 (link)]. Staining was performed according to the manufacturer's protocol. Briefly, slides were unmounted without prior destaining and sections were microwaved to achieve antigen unmasking. Cytoactiv^® HPV L1 capsid antibody was applied and slides were incubated for 30 minutes at 20°C. Following incubation, slides were treated with the detection reagents for 10 minutes and 3-amino-9-ethylcarbazole chromogen for 5 minutes. After counterstaining with hematoxylin, slides were mounted with Aquatex (Merck, Darmstadt, Germany) and a cover slip. Slides with a distinct nuclear staining for at least one epithelial cell were scored as positive. The slides were reviewed by 2 (link) pathologists for final diagnosis.

Choi Y.J., Lee A., Kim T.J., Jin H.T., Seo Y.B., Park J.S, & Lee S.J. (2018). E2/E6 ratio and L1 immunoreactivity as biomarkers to determine HPV16-positive high-grade squamous intraepithelial lesions (CIN2 and 3) and cervical squamous cell carcinoma. Journal of Gynecologic Oncology, 29(3), e38.

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Immunofluorescence Staining of ThinPrep Slides

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ThinPrep slides (Hologic Inc.) were immersed in 1X PBS for 5 minutes and then permeabilized with 0.2% PBS-T at room temperature for 30 minutes. After washing with 1X PBS, sections were blocked with 2% goat serum for 1 hour and then incubated with the primary antibody mixture, wherein MRS was diluted at a ratio of 1:250 and CD45 at a ratio of 1:100 in PBS, for 90 min. After washing, sections were incubated with a 1:1000 diluted secondary antibody mixture containing anti-Rabbit-AF488 and anti-Mouse-AF555 at room temperature for 1 hour and then reacted with 4′6-diamidino-2-phenylindole for 1 minute to counterstain the nucleus. Slides were mounted with coverslips using ProLong Gold Antifade Reagent® (P36930, Invitrogen, Carlsbad, CA, USA) and slides were shaded and stored frozen at −20℃. Stained slides were observed using a Carl-Zeiss Imager M2 fluorescence microscope (Imager M2, Carl Zeiss, Oberkochen, Germany) and a Carl-Zeiss LSM 750 confocal microscope (Carl Zeiss), and images were analyzed using ZEN-lite software (Carl-Zeiss).

Lee J.M., Kim T., Kim E.Y., Kim A., Lee D.K., Kwon N.H., Kim S, & Chang Y.S. (2019). Methionyl-tRNA Synthetase is a Useful Diagnostic Marker for Lymph Node Metastasis in Non-Small Cell Lung Cancer. Yonsei Medical Journal, 60(11), 1005-1012.

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Liquid-based NSCLC Specimens for ICC and Molecular Testing

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A consecutive series of NSCLC patients evaluated in the Department of Pathology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences, Beijing between November 2013 and October 2016 comprised the study cohort. Specimens from the patients were selected for analysis on the following basis: (1) fine-needle aspirate (FNA) specimens were able to be obtained from metastatic lesions; (2) at least 15 mL of PreservCyt solution (Hologic Inc., Marlborough, MA, USA) was left in the specimen bottles; and (3) more than 100 tumor cells were present in Papanicolaou-stained ThinPrep slides (Hologic Inc, Marlborough, MA, USA) [17 ]. The liquid-based materials were stored at 4°C and submitted for ICC and molecular testing within 3 months.
The study protocol was reviewed and approved by the ethics committee of the National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences. All patients provided informed consent to participate in the study.

Guo H.Q., Jia J., Zhao L.L., Zhao H., Wang C., Sun Y., Ying J.M., Guo L., Cao J, & Zhang Z.H. (2018). Application of Ventana immunocytochemical analysis on ThinPrep cytology slides for detection of ALK rearrangement in patients with advanced non–small-cell lung cancer. BMC Cancer, 18, 1277.

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Trichomonas Identification in Urine Cytology

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Our cytopathology archives were queried for urine cytology specimens over a 30-year period from 1985 to 2015. Specimens with Trichomonas were identified using a keyword search. Clinical information from men who had Trichomonas identified by urine cytology was obtained retrospectively from the electronic medical record. If available, Pap-stained ThinPrep slides (Hologic, Bedford, Mass) were reviewed. The following parameters were recorded: the number of organisms per 10 high-power fields (HPFs) and per 1 HPF, morphologic characteristics, and acute inflammatory reaction (defined as none [mild to few neutrophils], moderate [many neutrophils], or severe [numerous neutrophils]).

Doxtader E.E, & Elsheikh T.M. (2017). Diagnosis of trichomoniasis in men by urine cytology. Cancer cytopathology, 125(1).

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Bladder Cancer Cell Culture and Preservation

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High (HT1197; ATCC) and low (RT112, Sigma-Aldrich) grade urinary bladder carcinoma epithelium cells were cultured in 1:1 mixture of DMEM and Hams-F12 medium supplemented with 5% fetal bovine serum and 2 mM LGlutamine. Flasks were maintained in a humidified atmosphere with 5% CO

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at 37

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C. When the cells reached 80% confluency, the culture medium was removed, and the cells were rinsed with sterile PBS. Trypsin-EDTA (0.5%) was added to the flask, which was incubated at 37

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C until the cells had detached. An equal volume of 5% serum-containing medium was added to the flask to neutralise the trypsin enzyme. The cells were collected in medium and transferred into a sterile container, and centrifuged at 1200 rpm for 5 min. The supernatant was removed, and the cell pellet was resuspended in fresh medium. This solution was centrifuged at 1200 rpm for 5 min, and the medium was decanted and resuspended in 1 mL PBS. This step was repeated and the cell pellets were resuspended into a vial containing 20 mL of a methanol-based fixative (PreservCyt; Hologic, Marlborough, MA, USA) and left at room temperature for 15 min. The vial was inserted into a ThinPrep 2000 (T2; Hologic, Marlborough, MA, USA) machine, and the cells were transferred to a CaF

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(Raman Grade; Crystran, UK) slide, or a glass slide (ThinPrep slide; Hologic, Marlborough, MA, USA). No coverslips were applied.

O’Dwyer K., Domijan K., Dignam A., Butler M, & Hennelly B.M. (2021). Automated Raman Micro-Spectroscopy of Epithelial Cell Nuclei for High-Throughput Classification. Cancers, 13(19), 4767.

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FNA Specimen Processing for Cytology

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For FNA specimens, both direct air-dried (Diff-Quik stain) and alcohol-fixed (Papanicolaou stain) smears were prepared. The aspiration needles were then rinsed in CytoRich Red solution (Thermo Scientific, Hanover Park, IL), which was used to prepare a ThinPrep slide (Hologic, Marlborough, MA), and a CB, either a Cellient CB on the Cellient automated cell block system for low cellularity specimens (Hologic, Marlborough, MA) or a traditional HistoGel-based formalin-fixed paraffin embedded CB for cases with a significant amount of cell pellet. An aliquot of FNA specimen was set aside in PreservCyt solution at room temperature for potential high-risk HPV testing.

Abi-Raad R., Prasad M.L., Gilani S., Garritano J., Barlow D., Cai G, & Adeniran A.J. (2020). Quantitative Assessment of p16 Expression in FNA Specimens From Head and Neck Squamous Cell Carcinoma and Correlation With HPV Status. Cancer cytopathology, 129(5), 394-404.

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