C57bl 6 wt

Specific-pathogen-free animals were bred and maintained at the animal facilities of the Department of Biomedicine (University of Basel, Switzerland). The animal experiments received the approval of the Cantonal Veterinary Office of the city of Basel, Switzerland. Organ donors for in vitro experiments were 7–17 weeks of age. For in vivo experiments, age- and sex-matched animals were used. Animals were 8–11 weeks of age at the initiation of in vivo experiments.
C57BL/6 WT and Rag2^−/− mice^{44 (link)} were purchased from Janvier Labs and Taconic, respectively. RORc(γt)-Cre^tg mice^{45 (link)} and Rag2^−/−Ly5.1 were ordered from The Jackson Laboratory. Rptor^fl/fl and Rictor^fl/fl mice^{46 (link)} were kindly provided by M.N.H. (Biozentrum, University of Basel).
To generate Rptor^ΔRORγt mice, RORc(γt)-Cre^tg mice were crossed with Rptor^fl/fl mice and then bred to Rag2^−/− mice. To receive Rictor^ΔRORγt mice, RORc(γt)-Cre^tg mice were bred to Rictor^fl/fl mice and backcrossed to Rag2^−/− mice. In experiments with Rptor^ΔRORγt mice and Rictor^ΔRORγt mice, we used Cre⁻ littermates as controls. To generate Rag2^−/−Ly5.1/Ly5.2 mice, Rag2^−/− mice were mated with Rag2^−/−Ly5.1 mice. Rorγt^fm+ mice were received by breeding RORc(γt)-Cre^tg mice to Rosa26R^eYFP/+ mice^{45 (link),47 (link)}. Rorγt^fm+ mice were backcrossed to Rag2^−/− mice.

Age- and sex-matched animals were used for the experiments. C57BL/6 WT were obtained from Janvier (Le Genest Saint Isle, France), and C57BL/6 Cyld^−/− mice were kindly provided by Dr. Ramin Massoumi (Department of Laboratory Medicine, Malmö, Sweden) (9 (link)). The C57BL/6 Cyld^−/− mice were backcrossed with C57BL/6 mice for 10 generations. All animals were kept under conventional conditions in an isolation facility of the Otto-von-Guericke University Magdeburg. Experiments were approved and supervised by local governmental institutions.