Sodium cacodylate trihydrate

HPLC grade acetone, ethanol, and deionized water, phosphate-buffered saline (PBS) at a 10× solution, electron microscopy grade glutaraldehyde (GA) 25% solution, D-saccharose, glycine, biotin-free and molecular biology grade albumin fraction V (BSA), and sodium cacodylate trihydrate were obtained from Carl Roth GmbH + Co. KG, Karlsruhe, Germany. 0.01% poly(L-lysine) solution (PLL, M_r = 150,000–300,000, sterile-filtered, and cell-culture tested) was purchased from Sigma-Aldrich Chemie Gmbh, Munich, Germany. Sample support microchips with a central silicon nitride (SiN) membrane window of a dimension of 150 × 400 μm and a thickness of 50 nm were custom-made by DENSsolutions B.V. (Delft, Netherlands). The chips had outer dimensions of 2.00 × 2.60 × 0.30 mm³ fitting into the wells of a standard 96-well plate.

SKBR3 cells were obtained from ATCC. Biotin and fluorescein conjugated Anti-HER2 Affibody Molecule (HER2-AFF-B, termed: (ZHER2:477)2) was purchased from Affibody AB, Bromma, Sweden. Dulbecco’s Phosphate Buffered Saline (DPBS), Dulbecco’s Modified Eagle’s Medium GlutaMAX^TM with high glucose and pyruvate (DMEM), Fetal Bovine Serum, certified, heat inactivated (FBS), Minimum Essential Medium Non-Essential Amino Acids (NEAA) 100× solution, Phosphate Buffered Saline (PBS) 10× solution, CellStripper, Normal Goat Serum (GS), and Quantum Dot (QD) Qdot 655 Streptavidin Conjugate (Strep-QD) were from Fisher Scientific GmbH, Schwerte, Germany. D-Saccharose, Glycine, biotin free and molecular biology grade Albumin Fraktion V (BSA), and Sodium Cacodylate Trihydrate were from Carl Roth GmbH + Co. KG, Karlsruhe, Germany. Electron microscopy grade Formaldehyde (FA) 16% solution was from Science Services GmbH, Munich, Germany. HPLC grade deionized Water, 0.01% poly-L-lysine (PLL) solution (mol wt 70,000–150,000), Sodium Tetraborate, Boric Acid, and Fibronectin-like Engineered Protein Polymer-plus (FLEP), were from Sigma-Aldrich Chemie Gmbh, Munich, Germany. 35 mm-cell culture dishes with uncoated glass bottom were from Greiner bio-one, Frickenhausen, Germany (CELLviewTM four compartments), and from ibidi, Munich, Germany (μ-Dish, with imprinted 50 or 500 μm relocation grid).