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Sciclone liquid handling robot

Manufactured by PerkinElmer
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Sourced in United States

The Sciclone liquid handling robot is a versatile laboratory instrument designed for automated liquid handling tasks. It features precise liquid transfer capabilities and can be configured with various pipetting tools to accommodate a wide range of applications. The core function of the Sciclone is to automate liquid handling processes, enabling efficient and accurate sample preparation, dilution, and distribution. The product's technical specifications and capabilities are presented without interpretation or extrapolation on its intended use.

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Lab products found in correlation

Truseq stranded mrna reagents, by Illumina (7 mentions) Fragment analyzer, by Agilent Technologies (5 mentions) Hiseq 3000 4000 sbs kit reagents, by Illumina (3 mentions) Truseq sbs kit v4 reagents, by Illumina (3 mentions) Hiseq 2500, by Illumina (3 mentions) Truseq sr cluster kit v4 reagents, by Illumina (3 mentions) Hiseq 3000 4000 sr cluster kit reagents, by Illumina (2 mentions) Bcl2fastq2 conversion software, by Illumina (2 mentions) Hiseq 4000, by Illumina (2 mentions) Smart seq v4 ultra low input rna reagents, by Takara Bio (1 mentions) Nextera xt dna library reagents, by Illumina (1 mentions) Rneasy plus mini kit, by Qiagen (1 mentions) Rnaeasy minielute spin column, by Qiagen (1 mentions) Anti cd3 and anti cd28 antibody, by Thermo Fisher Scientific (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Rnaeasy mini kit, by Qiagen (1 mentions) Fragment analyzer, by Thermo Fisher Scientific (1 mentions) Bcl2fastq conversion software, by Illumina (1 mentions) Dnase 1, by Qiagen (1 mentions) Hiseq sr cluster kit v4 reagents, by Illumina (1 mentions)

7 protocols using sciclone liquid handling robot

1

Transcriptomic Analysis of Primary Brain Endothelial Cells

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Ch25hfl/fl and Ch25hECKO female mice were injected with tamoxifen. Nine brains per genotype were pooled and primary brain microvascular endothelial cells (pMBMEC) were isolated and plated in a 96‐well plate (2 wells/brain). Confluent pMBMEC were left unstimulated or stimulated IL‐1β for 24 h. RNA of three wells was pooled to obtain one replicate for RNA sequencing. The Lausanne Genomic Technologies Facility performed the RNA‐seq. RNA quality was assessed on a Fragment Analyzer (Agilent Technologies), and all RNAs had a RQN between 8.7 and 10. RNA‐seq libraries were prepared from 500 ng of total RNA with the Illumina TruSeq Stranded mRNA reagents (Illumina) using a unique dual indexing strategy, and following the official protocol automated on a Sciclone liquid handling robot (PerkinElmer). Libraries were quantified by a fluorometric method (QubIT, Life Technologies) and their quality assessed on a Fragment Analyzer (Agilent Technologies).
Cluster generation was performed with 2 nM of an equimolar pool from the resulting libraries using the Illumina HiSeq 3000/4000 SR Cluster Kit reagents and sequenced on the Illumina HiSeq 4000 using HiSeq 3000/4000 SBS Kit reagents for 150 cycles (single end). Sequencing data were demultiplexed using the bcl2fastq2 Conversion Software (version 2.20, Illumina).
Ruiz F., Peter B., Rebeaud J., Vigne S., Bressoud V., Roumain M., Wyss T., Yersin Y., Wagner I., Kreutzfeldt M., Pimentel Mendes M., Kowalski C., Boivin G., Roth L., Schwaninger M., Merkler D., Muccioli G.G., Hugues S., Petrova T.V, & Pot C. (2023). Endothelial cell‐derived oxysterol ablation attenuates experimental autoimmune encephalomyelitis. EMBO Reports, 24(3), e55328.
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2

RNA-seq Library Preparation for Splenic and Bone Marrow NK Cells

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Total RNA was extracted from sorted cells using the RNeasy plus mini kit (Qiagen) according to the manufacturer's instructions. Clontech RNA-seq libraries were prepared for the splenic NK cell preparations. Double stranded cDNA for RNA-seq library preparation was generated using SMART-Seq v4 Ultra Low Input RNA reagents (Catalog Number 634888, Clontech) according to the protocol provided with the reagents beginning with 10 ng of total RNA and using 9 cycles of PCR. 150 pg of the resulting cDNA were used for library preparation with the Illumina Nextera XT DNA Library reagents (Catalog Number 15032354, Illumina) using the single cell RNA-seq library preparation protocol developed for the Fluidigm C1 (Fluidigm).
TruSeq stranded mRNA-seq libraries were prepared for the bone marrow-derived NK cells. RNA-seq libraries were prepared using 400 ng of total RNA and the Illumina TruSeq Stranded mRNA reagents (Illumina) according to the protocol supplied with the reagents and starting with 100ng of total RNA on a Sciclone liquid handling robot (PerkinElmer) using a PerkinElmer-developed automated script.
Library sequencing cluster generation was performed with the resulting libraries using the Illumina TruSeq SR Cluster Kit v4 reagents and sequenced on the Illumina HiSeq 2500 using TruSeq SBS Kit v4 reagents. Sequencing data were processed using the Illumina Pipeline Software version 1.82.
Castro W., Chelbi S.T., Niogret C., Ramon-Barros C., Welten S.P., Osterheld K., Wang H., Rota G., Morgado L., Vivier E., Raeber M.E., Boyman O., Delorenzi M., Barras D., Ho P.C., Oxenius A, & Guarda G. (2018). The transcription factor Rfx7 limits metabolism of NK cells and promotes their maintenance and immunity. Nature immunology, 19(8), 809-820.
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3

RNA Extraction and RNA-Seq Analysis in Mice Kidneys

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RNA from frozen half-kidneys of 72 mice were extracted and purified using RNAeasy MiniElute Spin Column (Qiagen). RNA quality was assessed on a Fragment Analyzer (Agilent Technologies). All RNAs had an RNA quality number (RQN) between 7.5 and 9.7. RNA-Seq libraries were prepared from 200 ng of total RNA with the Illumina TruSeq Stranded mRNA reagents (Illumina) using a unique dual indexing strategy and following the official protocol automated on a Sciclone liquid handling robot (PerkinElmer). Libraries were quantified by a fluorometric method (QubIT, Life Technologies) and their quality assessed on a Fragment Analyzer (Agilent Technologies). Clusters were generated with 2 nM of an equimolar pool from the resulting libraries using the Illumina HiSeq 3000/4000 SR Cluster Kit reagents. Sequencing was performed on the Illumina HiSeq 4000 using HiSeq 3000/4000 SBS Kit reagents for 150 cycles (single read). Sequencing data were demultiplexed, filtered for failed reads, and written to FASTQ files using the bcl2fastq2 conversion software (version 2.20, Illumina). Details for RNA-Seq reads mapping are described in Supplemental Methods.
Bignon Y., Wigger L., Ansermet C., Weger B.D., Lagarrigue S., Centeno G., Durussel F., Götz L., Ibberson M., Pradervand S., Quadroni M., Weger M., Amati F., Gachon F, & Firsov D. (2023). Multiomics reveals multilevel control of renal and systemic metabolism by the renal tubular circadian clock. The Journal of Clinical Investigation, 133(8), e167133.
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4

RNA-seq analysis of STING-deficient T cells

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For RNA sequencing naïve T cells were sorted from the spleens of wild-type mice and STINGgt/gt mice and expanded in the presence of anti-CD3 and anti-CD28 antibodies (eBioscience) for 2 days. Cells were rested 1 day in RPMI-1640 medium supplemented with 10% (v/v) FCS, 5% HEPES buffer (Amimed), 2% L-glutamine (Life Technologies) and Ciprofloxacin (Bayer Schering Pharma), without antibodies and stimulated with either DMSO or 125 μg/ml CMA overnight. Total RNA was isolated from the cells using the RNAeasy Mini Kit (Qiagen). RNA-seq libraries were prepared using 1000 ng of total RNA and the Illumina TruSeq Stranded mRNA reagents (Illumina) on a Sciclone liquid handling robot (PerkinElmer) using a PerkinElmer-developed automated script. Cluster generation was performed with the resulting libraries using the Illumina TruSeq SR Cluster Kit v4 reagents and sequenced on the Illumina HiSeq 2500 using TruSeq SBS Kit v4 reagents. Sequencing data were processed using the Illumina Pipeline Software version 1.82. Heat maps were produced from normalised expression data using Cluster 3.0 for computation and JTreeview for visualisation.
Gulen M.F., Koch U., Haag S.M., Schuler F., Apetoh L., Villunger A., Radtke F, & Ablasser A. (2017). Signalling strength determines proapoptotic functions of STING. Nature Communications, 8, 427.
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5

RNA Extraction and RNA-seq Library Preparation

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RNA was extracted using the Total RNA Isolation RNeasy Mini Kit with the DNAse I (QIAGEN), on-column digestion step. Snap-frozen pieces of tumor and normal tissue samples (≈30 mg) were directly submerged in 350 µl of RLT buffer (second RNA wash buffer with ethanol) supplemented with 40 µM dithiothreitol. Tissues were completely homogenized on ice using a pestle and passed through a 26G needle syringe 5×. Centrifugation was performed in a table-top centrifuge at 4 °C for 3 min at 18,213g before the supernatant was removed and directly used for RNA extraction.
RNA quality was assessed on a Fragment Analyzer (Agilent Technologies). RNA-seq libraries were prepared from 500 ng of total RNA with the Illumina TruSeq Stranded mRNA reagents using a unique dual indexing strategy and following the official protocol automated on a Sciclone liquid handling robot (PerkinElmer). Libraries were quantified by a fluorimetric method (QubIT, Life Technologies) and their quality assessed on a Fragment Analyzer.
Cluster generation was performed with the resulting libraries using Illumina HiSeq 3000/4000 PE Cluster Kit reagents. Libraries were sequenced on the Illumina HiSeq 4000 with HiSeq 3000/4000 SBS Kit reagents for 2× 150 cycles. Sequencing data were de-multiplexed with the bcl2fastq Conversion Software (v.2.20, Illumina).
Kraemer A.I., Chong C., Huber F., Pak H., Stevenson B.J., Müller M., Michaux J., Altimiras E.R., Rusakiewicz S., Simó-Riudalbas L., Planet E., Wiznerowicz M., Dagher J., Trono D., Coukos G., Tissot S, & Bassani-Sternberg M. (2023). The immunopeptidome landscape associated with T cell infiltration, inflammation and immune editing in lung cancer. Nature Cancer, 4(5), 608-628.
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6

Automated RNA-seq Library Preparation

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RNA‐seq libraries were prepared as described in Jan et al (2019). In brief, RNA quality was assessed on a Fragment Analyzer (Advanced Analytical Technologies, Inc., Ankeny, IA, USA). RNA‐seq libraries were prepared using 1,000 ng of total RNA and the Illumina TruSeq Stranded mRNA reagents (Illumina; San Diego, California, USA) on a Sciclone liquid handling robot (PerkinElmer; Waltham, Massachusetts, USA) using a PerkinElmer‐developed automated script. Cluster generation was performed with the resulting libraries using the Illumina TruSeq SR Cluster Kit v4 reagents and sequenced on the Illumina HiSeq 2500 using TruSeq SBS Kit v4 reagents. Sequencing data were processed using the Illumina Pipeline Software version 2.20.
Fujita S., De Bellis D., Edel K.H., Köster P., Andersen T.G., Schmid‐Siegert E., Dénervaud Tendon V., Pfister A., Marhavý P., Ursache R., Doblas V.G., Barberon M., Daraspe J., Creff A., Ingram G., Kudla J, & Geldner N. (2020). SCHENGEN receptor module drives localized ROS production and lignification in plant roots. The EMBO Journal, 39(9), e103894.
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7

RNA Extraction and RNA-seq Library Preparation from Fly Heads

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Fly heads were quickly collected at ZT16 using liquid nitrogen. Total RNA was isolated using Trizol Reagent (Gibco) according to the manufacturer’s instructions to extract total RNA. RNA quality was evaluated on a Fragment Analyzer (Agilent Technologies, Inc., Santa Clara, CA 95051, USA) and the RQN were between 8.5 and 9.7. RNA-seq libraries were prepared using 500 ng of total RNA and Illumina TruSeq Stranded mRNA reagents (Illumina; San Diego, California, USA) on a Sciclone liquid handling robot (PerkinElmer; Waltham, Massachusetts, USA) using a PerkinElmer-developed automated script. Cluster generation was performed with the resulting libraries using the Illumina HiSeq SR Cluster Kit v4 reagents and sequenced on the Illumina HiSeq 2500 using HiSeq SBS Kit v4 reagents. Sequencing data were demultiplexed using the bcl2fastq Conversion Software (v. 2.20, Illumina; San Diego, California, USA).
Mariano V., Kanellopoulos A.K., Aiello G., Lo A.C., Legius E., Achsel T, & Bagni C. (2023). SREBP modulates the NADP+/NADPH cycle to control night sleep in Drosophila. Nature Communications, 14, 763.
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