Immunohistochemical studies were performed essentially as previously described [7 ]. Briefly, prior to immunostaining tissue underwent deparaffinization in xylene, followed by antigen retrieval, and blocking of endogenous peroxidase. The immunohistochemical staining was done using a standard labeled streptavidin-biotin method (VECTASTAINVR ABC Kit, # PK-4001, Vector Laboratories, CA, USA) followed by 3,3′-diaminobenzidine enzymatic development. The following antibodies were used: anti-PRMT1 antibody (Millipore, #07-404, 1:100 dilution) and anti-MYCN antibody (Santa Cruz, #sc-53993, 1:100 dilution). Normal rabbit or mouse IgG serves as the negative control. Slides were imaged with an Olympus VS120 slide scanner system. Immunoreactivity for PRMT1 and MYCN was independently scored by two investigators (JH and LL), both blinded as to the identity of each specimen, based on the previously described scoring method [7 ]. The statistical analysis was done by a two-tailed Fisher's exact test with a 95% confidence interval; P values < 0.05 were considered significant.
Anti prmt1 antibody
Manufactured by Merck Group
The Anti-PRMT1 antibody is a laboratory research tool used to detect and study the protein arginine N-methyltransferase 1 (PRMT1) in various biological samples. PRMT1 is an enzyme involved in the post-translational modification of proteins through the addition of methyl groups to arginine residues. This antibody can be used in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to investigate the expression, localization, and function of PRMT1 in cells and tissues.
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Lab products found in correlation
Vs120 slide scanner system, by Olympus (1 mentions)
Anti mycn antibody, by Santa Cruz Biotechnology (1 mentions)
Vectastainvr abc kit, by Vector Laboratories (1 mentions)
Anti dimethyl arginine antibody, by Merck Group (1 mentions)
Anti flag antibody, by Merck Group (1 mentions)
Anti vinculin antibody, by Merck Group (1 mentions)
2 protocols using anti prmt1 antibody
1
Immunohistochemical Analysis of PRMT1 and MYCN
2
Detection of NS5 Protein by Western Blot
Check if the same lab product or an alternative is used in the 5 most similar protocols
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To detect NS5 in Western blots, a rabbit polyclonal antiserum was applied that was raised against the recombinant, purified full-length protein (Eurogentec). Anti-Vinculin antibody and anti-FLAG antibody were purchased from Sigma. Anti-PRMT1 antibody and anti-dimethyl-arginine antibody were purchased from Millipore. Secondary antibodies were purchased from GE Healthcare and LICOR. Western blots were performed using standard protocols and by following the instructions of the manufacturers.
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