Primary cardiomyocytes were exposed to A/R treatment for 0, 4, 8 or 12 h, and the medium was collected for LDH activity measurement using an LDH Quantification kit (cat no. K726-500; BioVision, Inc.) according to the manufacturer's protocol.
Ldh quantification kit
Manufactured by Abcam
Sourced in United States
The LDH Quantification kit is a laboratory assay designed to measure the activity of the enzyme Lactate Dehydrogenase (LDH) in biological samples. LDH is a common marker used to assess cell health and cytotoxicity. The kit provides a colorimetric readout that can be quantified using a spectrophotometer.
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3 protocols using ldh quantification kit
1
Cardiomyocyte A/R Damage Quantification
2
Glycolysis Evaluation in Cells
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Treated cells were harvested and washed with PBS twice. To evaluate the glycolysis of cells, relative glucose consumption, lactate generation and LDH activity were detected by using Amplex Red Glucose/Glucose Oxidase assay kit (Molecular Probes; Thermo Fisher Scientific, Inc. USA), Lactate Assay Kit (BioVision, Milpitas, CA, USA) and LDH Quantification Kit (Biovision), respectively.
3
Assessing Cell Viability and LDH Secretion
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The cell viability and LDH secretion were quantified in the SH-SY5Y cell line and cortical neuron cultures using the Cell Counting Kit-8 (Dojindo, Molecular Technologies, MD, USA) and the LDH quantification kit (Biovision, CA, USA) as per the manufacturer’s instructions.
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