The structural characteristics and average particle sizes were determined using transmission electron microscopy (TEM) on a Phillips CM-12 operated at 100 kV. Samples for TEM studies were prepared by placing a drop of the irradiated solutions on a copper TEM grid. Prior to microscopy, the samples were allowed to dry naturally on the grids for several hours. Fourier transform infrared spectroscopy (FT-IR) spectra were recorded in the range 650–4,000 cm−1 on a Spectrum 400 FTIR spectrophotometer (Perkin Elmer) with the samples embedded in KBr pellets. The crystal structure of the Fe3O4 nanoparticles was characterized using X-ray diffractometry (XRD), performed at a scanning rate of 0.025°/0.1 s over the 2θ range 20–90° on a D8 Advance diffractometer (Bruker) with graphite-monochromatized Cu-Kα radiation (λ = 1.5406 Å). The magnetic properties of nanoparticles were determined at 25°C using a model 9500 (LDJ) computerized vibrating sample magnetometer.
Spectrum 400 ftir spectrophotometer
Manufactured by PerkinElmer
Sourced in United States
The Spectrum 400 FTIR spectrophotometer is an analytical instrument designed to measure the infrared absorption spectrum of a sample. It uses Fourier-transform infrared (FTIR) technology to analyze the composition and structure of materials by detecting the infrared absorption patterns of their chemical bonds.
Automatically generated - may contain errors
Lab products found in correlation
D8 advance diffractometer, by Bruker (1 mentions)
Vario micro cube elemental analyzer, by Elementar (1 mentions)
Hacat, by National Centre for Cell Science (1 mentions)
Ribonuclease a, by Merck Group (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
400 mhz spectrometer, by Bruker (1 mentions)
Spotlight 400, by PerkinElmer (1 mentions)
Lambda 900 spectrophotometer, by PerkinElmer (1 mentions)
Nano zs90, by Malvern Panalytical (1 mentions)
Laser scanning confocal microscope, by Nikon (1 mentions)
Fl 8500 fluorescence spectrophotometer, by PerkinElmer (1 mentions)
5 protocols using spectrum 400 ftir spectrophotometer
1
Characterization of Fe3O4 Nanoparticles
2
FT-IR Analysis of Sophoricoside Forms
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
FT-IR absorption spectra were recorded with a Spectrum 400 FT-IR spectrophotometer (PerkinElmer, USA). The spectra were used to identify the functional groups in the four forms of sophoricoside. The spectra were collected in the range 650–4000 cm−1 with a 4 cm−1 resolution. An attenuated total reflectance sampling accessory with a diamond window was used.
3
Synthesis and Characterization of Novel Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All chemicals and anhydrous solvents used in this work were purchased from commercial sources and used without further purification. FTIR spectra were recorded in a PerkinElmer Spectrum 400 FT-IR spectrophotometer. 1H and 31P{1H} NMR spectra were recorded on Bruker 400 MHz spectrometer. Elemental analyses were carried out using an Elementar Vario Micro Cube elemental analyzer. ESI-MS analysis was performed using a Bruker Impact ESI-Q-TOF system. Theoretical calculations of PM6 semiempirical molecular orbital method were carried out with Gaussian 09. A549 (human lung cancer cell line), KB (human oral cancer cell line) and HaCaT (human skin keratinocyte cell line) were procured from National Centre for Cell Science (NCCS), Pune. MTT [(3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrasodium bromide] was purchased from SigmaAldrich, USA. Ethidium homodimer-1 in 2 mL pbs, propidium iodide, Ribonuclease A were also purchased from SigmaAldrich.
4
ATR-FTIR Imaging of Wood Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ATR-FTIR images were produced from stem cross-sections by a mid-IR light (1,800–850 cm–1) at a 4 cm–1 spectral resolution using a Spotlight 400 FTIR imaging system coupled to a Spectrum 400 FTIR spectrophotometer (PERKIN ELMER, Wellesley, USA). According to the manufacturer’s procedure, the wood section was pushed by pressure into direct contact with the tip of the 600-µm diameter plane Germanium crystal. Supplementary Figure 1
5
Characterization of MOF Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The characterization method is a slight modification of what we have reported in our previous articles [54 (link)]. The MOF was diluted with ultra-pure water and dropped directly onto the copper network for TEM observation and elemental mappings. The MOF was diluted with ultra-pure water and then dropped into the conductive glass and dried and sprayed with gold for scanning electron microscope observation. The zeta potentials and particle size of MOF nanoparticles were detected by Malvern Nano ZS90. Fourier transforms infrared (FT-IR) spectra of functionalized MOF were obtained by an Spectrum 400FT-IR spectrophotometer (PerkinElmer, USA), as reported previously [55 (link)]. Lambda 900 spectrophotometer (PerkinElmer, USA) was used to measure the UV–Vis absorption spectra of ICG-CpG@MOF. FL-8500 fluorescence spectrophotometer (PerkinElmer, USA) was used to record the fluorescence excitation and emission spectra of ICG-CpG@MOF, as we reported earlier [46 (link)]. A Nikon confocal laser scanning microscope was used to observe the internalization of fluorescently labeled MOF with a slight change from previous article [56 (link)]. The photothermal effects of ICG-CpG@MOF were evaluated using reported methods [57 (link),58 (link)].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!