C57BL/6J mice and C57BL/10ScNJ mice (carrying a spontaneous deletion of the Tlr4 gene) were obtained from The Jackson Laboratory and bred in the animal facility at Leloir Institute. All mice were bred under specific pathogen-free conditions and were used at 8–10 week of age. B16-F1 melanoma (ATCC CRL-6323), syngeneic from C57BL/6 mice was a kind gift from Dr José Mordoh´s lab and was cultured at 37°C under 5% CO2 in endotoxin-free RPMI 1640 medium supplemented with 10% FBS (Gibco; Grand Island, NY, USA), penicillin and streptomycin, 1 mM pyruvate and 4 mM L-glutamine. OVA-expressing B16-F1 melanoma (B16-OVA) was kindly provided by Dr Paolo Dellabona [41 (link)] and was cultured in the same media with the addition of 100 μg/ml hygromycin B (Roche Diagnostics, Mannheim, Germany).
B16 f1 melanoma
Manufactured by American Type Culture Collection
Sourced in United States
B16-F1 melanoma is a murine melanoma cell line derived from the B16 melanoma. It is a widely used model for studying melanoma and its progression.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
C57bl 6j mice, by Jackson ImmunoResearch (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
C57bl 10scnj mice, by Jackson ImmunoResearch (1 mentions)
Hygromycin b, by Roche (1 mentions)
Dulbecco modified eagle medium (dmem), by Promega (1 mentions)
Pdon 5 neo vector, by Takara Bio (1 mentions)
Ht1080, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
H1299, by American Type Culture Collection (1 mentions)
Ht1080, by American Type Culture Collection (1 mentions)
4 protocols using b16 f1 melanoma
1
Murine Melanoma Propagation and Culture
2
Syngeneic Melanoma Tumor Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
C57BL/6J mice were obtained from The Jackson Laboratory and bred in the animal facility at Leloir Institute. All mice were bred under specific pathogen-free conditions and were used at 8–10 week of age. B16-F1 melanoma (ATCC CRL-6323, Manassas, Virginia, United States), syngeneic from C57BL/6 mice was a kind gift from Dr José Mordoh´s lab and was cultured at 37 °C under 5% CO2 in endotoxin-free RPMI 1640 medium supplemented with 10% FBS (Gibco; Grand Island, NY, USA), penicillin and streptomycin, 1 mM pyruvate and 4 mM l -glutamine.
3
APN/CD13 Overexpression in B16-F1 Melanoma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HT1080, B16-F1 melanoma, H1299 and A549 cells were obtained from American Type Culture Collection (ATCC; Manassas, VA, USA). PC14 cells were obtained from Immuno-Biological Laboratories (Gunma, Japan). HT1080, B16-F1, and A549 cells were cultured in DMEM (Gibco, Grand Island, NY, USA), and H1299 and PC14 cells were cultured in RPMI 1640 Medium (Gibco) supplemented with 10% FBS and 1% penicillin-streptomycin. All cells were incubated at 37°C in an atmosphere containing 5% CO2.
A retroviral vector (pDON-5neoCD13; Takara Bio, Shiga, Japan) containing APN/CD13 cDNA and a control pDON-5neo vector were transfected into B16-F1 melanoma cells according to the manufacturer’s protocol. Transfectants resistant to 1 mg/mL G-418 (Promega, Madison, WI, USA) were selected and maintained in DMEM with 10% FBS and 1 mg/mL G-418. From these B16-F1 transfectants, one cell line that expressed high levels of APN/CD13 (APN-B16 cells) and another cell line that did not express APN/CD13 (control-B16 cells) were selected. The expression levels of APN/CD13 were determined by flow cytometry and real-time PCR.
A retroviral vector (pDON-5neoCD13; Takara Bio, Shiga, Japan) containing APN/CD13 cDNA and a control pDON-5neo vector were transfected into B16-F1 melanoma cells according to the manufacturer’s protocol. Transfectants resistant to 1 mg/mL G-418 (Promega, Madison, WI, USA) were selected and maintained in DMEM with 10% FBS and 1 mg/mL G-418. From these B16-F1 transfectants, one cell line that expressed high levels of APN/CD13 (APN-B16 cells) and another cell line that did not express APN/CD13 (control-B16 cells) were selected. The expression levels of APN/CD13 were determined by flow cytometry and real-time PCR.
4
Syngeneic Tumor Models for Cancer Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The B16-F1 melanoma and E.G7 OVA-expressing thymoma47 (link) were obtained from the American Type Culture Collection, Manassas, VA. The B16.OVA melanoma48 (link) was kindly provided by Dr Roslyn Kemp, Trudeau Institute. EL-4 thymoma cells were a gift from Prof. Michael Berridge, Malaghan Institute of Medical Research. All cell lines were maintained in complete Iscove's modified Dulbecco medium as described.19,49 (link) Extended in vitro passaging was avoided. Mice were injected with either 105 (B16-F1, B16.OVA) or 5 × 105 (E.G7-OVA, EL-4) cells s.c. into the flank. Tumor size and survival were calculated as described.19 (link)
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!