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Nf κb

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NF-κB is a protein complex that acts as a transcription factor. It plays a key role in regulating the immune response, cell survival, and inflammation.

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Lab products found in correlation

Synergy 2, by Agilent Technologies (1 mentions) Oneglo assay, by Promega (1 mentions) One glo reagent, by Promega (1 mentions) Synergy 2 plate reader, by Agilent Technologies (1 mentions)

Spelling variants of this product

PGF-NF-κB-mCMV-EF1-Puro (4 citations) NF-κB/Jurkat/GFP Transcriptional Reporter cells (2 citations) NF-κB/Jurkat/GFPTM Transcriptional Reporter cell line (2 citations) PGreenFire1-NF-κB (EF1α-puro) lentivector (2 citations) NF-κB/Jurkat/GFP™ Transcriptional Reporter cell line (2 citations)

2 protocols using nf κb

1

NF-κB Activation Monitoring in SUM159

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Lentiviral particles containing luciferase reporter construct driven by NF-κB (system biosciences) were obtained and transfected into SUM159 cell line. Briefly, 4 copies of NF-κB TRE sequences “GGGACTTTCC” were inserted upstream of minimal essential CMV (mCMV) promoter which drives GFP-T2A-luciferase. Polyprotein is cleaved at the T2A site to give rise to GFP and Luciferase. Cell lines were plated at a density of 3000 cells per well in a clear bottom, white 96-well plate and treated with the indicated concentration with sulforaphane. Two hours following incubation with sulforaphane, TNF-α concentration in media was brought to 50 ng/ml. After 6 h Luciferase activity was measured according to manufacturer’s instructions using oneGlo assay (Promega) on Bio-Tek Synergy 2 plate reader.
Burnett J.P., Lim G., Li Y., Shah R.B., Lim R., Paholak H.J., McDermott S.P., Sun L., Tsume Y., Bai S., Wicha M.S., Sun D, & Zhang T. (2017). Sulforaphane enhances the anticancer activity of taxanes against triple negative breast cancer by killing cancer stem cells. Cancer letters, 394, 52-64.
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2

NF-κB Luciferase Assay in BT474 Cells

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Lentiviral particles containing luciferase reporter construct driven by NF-κB (System Biosciences) were obtained and transfected into BT474 and BT474 PTEN− LTT cells. Two hours following incubation with SF, TNF-α concentration in media was brought to 50 ng/ml. After 6 hr Luciferase activity was measured according to manufacturer’s instructions with oneGlo reagent (promega) on Synergy 2 plate reader (BioTek).
Burnett J.P., Korkaya H., Ouzounova M.D., Jiang H., Conley S.J., Newman B.W., Sun L., Connarn J.N., Chen C.S., Zhang N., Wicha M.S, & Sun D. (2015). Trastuzumab resistance induces EMT to transform HER2+ PTEN− to a triple negative breast cancer that requires unique treatment options. Scientific Reports, 5, 15821.
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