T4375

Twenty-four hours after surgery, the animals were sacrificed, and the brains were collected with cerebellum and overlying membranes being removed. The brains were sectioned into 8 slices using a brain-sectioning block, each with 2 mm in thickness. The brain slices were incubated for 30 min in a 0.1% solution of 2,3,4-triphenyltetrazolium chloride (TTC) (T4375, Sigma-Aldrich, MI, USA) at 37 °C. The sections were scanned using HP Scanjet G3110 photo scanner (HP Inc., CA, USA), and the infarct size was analyzed using ImageJ software (NIH, DC, USA). Edema-corrected lesion was calculated accordingly [9 (link)].

Twenty-four hours after surgery, the animals were sacrificed, and the brains were collected with cerebellum and overlying membranes being removed. The brains were sectioned into 8 slices using a brain-sectioning block, each with 2 mm in thickness. The brain slices were incubated for 30 min in a 0.1% solution of 2,3,4-triphenyltetrazolium chloride (TTC) (T4375, Sigma-Aldrich, MI, USA) at 37 °C. The sections were scanned, and the infarct size was analysed using an image analyser system (HP Scanjet G3110, HP Inc, CA, USA). Calculation of oedema-corrected lesion was performed as described previously [16 (link)].

2,3,5-Triphenyltetrazolium chloride (TTC) staining is a convenient procedure for detection of brain infarcts in stroke. 24 h after surgery, the brains were collected. After removing the cerebellum and overlying membranes, the brains were sectioned into 8 slices with 2 mm in thickness. The slices were then incubated in a 0.1% solution of TTC (T4375, Sigma-Aldrich, MI, USA) for 30 min at 37 °C. After the slices were scanned and analysed using an image analyser system (HP Scanjet G3110, HP Inc, CA, USA), infarct volume was calculated with a correction of oedema as described previously^{26 (link)}.
The motor functions in animals after surgery were evaluated using a Rotarod produced by Ugo Basile, Gemonio, Italy. Before operation, all rats received 3 training trials with 15-min intervals for 5 consecutive days. The Rotarod was set to accelerate from 4 to 80 rpm within 10 min. The mean duration of time that the animals remained on the device 1 day before operation was recorded and set as baseline control. One day following surgery, the mean duration of latency was recorded and compared with baseline. Animals with the performance higher than baseline were excluded from the analysis.