Sst ires cre mice

Manufactured by Jackson ImmunoResearch

SST-IRES-Cre mice are a genetically modified mouse line that expresses Cre recombinase under the control of the somatostatin (Sst) gene regulatory sequences. The Cre recombinase enzyme facilitates site-specific recombination, allowing for targeted genetic manipulation in somatostatin-expressing cells.

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Lab products found in correlation

C57bl 6 mice, by Charles River Laboratories (2 mentions) Pv ires cre mice, by Jackson ImmunoResearch (2 mentions) C57bl 6j, by Jackson ImmunoResearch (2 mentions) Vgat ires cre mice, by Jackson ImmunoResearch (1 mentions) Ai9 reporter mice, by Jackson ImmunoResearch (1 mentions) Rosa26 loxp stop loxp cag tdtomato ai9 mice, by Jackson ImmunoResearch (1 mentions) Pv cre mice, by Jackson ImmunoResearch (1 mentions)

Spelling variants of this product

SST-Cre (36 citations) SST-IRES-Cre (32 citations) SST-cre mice (7 citations) Sst-IRES-Cre knock-in mice (3 citations) SST-ires-cre knock-in homozygous mice (2 citations) SST-IRES-Cre mice (Ssttm2.1(cre)Zjh/J) (2 citations)

6 protocols using sst ires cre mice

Transgenic Zebrafish and Mouse Lines

Cited 1 time

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The Tg(pomca:EGFPras)^fr38Tg, Tg(pomca:KaITA4)^fr39Tg, Tg(sst1.1:EGFPras)^fr40Tg, and Tg(pomca(pit):CFP-nfsb;cmlc2:GFP)^fr41Tg zebrafish lines were generated during the course of this study (Supplemental Experimental Procedures). The Tg(UAS-E1b:nfsb-mCherry)^c264 line was previously described (Davison et al., 2007 (link)). All zebrafish experiments were approved by the national animal welfare committees (LANUV Nordrhein-Westfalen; 8.87–50.10.31.08.129; 84–02.04.2012.A251; 84–02.04.2012.A390; City of Cologne; 576.1.36.6.3.01.10 Be) and the University of Cologne. C57BL/6 mice were purchased from Charles River Laboratories, Sst-IRES-Cre mice (Taniguchi et al., 2011 (link)) from The Jackson laboratory (stock number 013044). For generation of R26-fl-rxΔ-ZsGreen transgenic and Sst-IRES-Cre; R26-fl-rxΔ-ZsGreen double transgenic mice, see Supplemental Experimental Procedures. Mice were housed under controlled environment in a 12h light/dark cycle, with food and water ad libitum. The procedures were approved by the Bezirksregierung (local authority in Cologne, Germany).

Löhr H., Hess S., Pereira M.M., Reinoß P., Leibold S., Schenkel C., Wunderlich C.M., Kloppenburg P., Brüning J.C, & Hammerschmidt M. (2018). Diet-Induced Growth Is Regulated via Acquired Leptin Resistance and Engages a Pomc-Somatostatin-Growth Hormone Circuit. Cell reports, 23(6), 1728-1741.

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Transgenic Zebrafish and Mouse Lines

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Mouse GABA Receptor Knock-In Models

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All experimental procedures were approved by the University of California Berkeley Animal Care and Use Committee, in compliance with all relevant regulatory standards.
Unless otherwise noted, mice were group housed by sex with free access to food and water. Mice aged 2 weeks - 6 months were used as indicated. Mice of both sex were used for electrophysiology experiments, and no influence of sex was observed on any results. Male mice were used for behavioral experiments, and were food restricted for 2 days prior to behavioral testing.
Mouse strains used in this study include C57BL/6J, referred to as wild type (WT) either obtained from Jackson Laboratory, or as litter-mate controls from genetically modified strain breeding; α1-GABAR-E125C knock in mice, referred to as α1-E125C-KI, generated by Lin et al., 2015 (link), since deposited and available from Jackson Laboratory; α5-GABAR-E125C knock in mice, referred to as α5-E125C-KI, generated in this paper by the UC Davis mouse biology program; SOM-Cre-α5-E125C-KI mice generated by crossing SST-IRES-CRE mice from Jackson Laboratory with α5-E125C mice; PV-Cre-α5-E125C-KI mice generated by crossing PV-IRES-CRE mice obtained from Jackson Laboratory with α5-E125C mice; α5-GABAR knockout mice (α5-KO) obtained from Uwe Rudolph at McLean Hospital, under agreement with the University of Zurich.

Davenport C.M., Rajappa R., Katchan L., Taylor C.R., Tsai M.C., Smith C.M., de Jong J., Arnold D.B., Lammel S, & Kramer R.H. (2020). Relocation of an extrasynaptic GABAA receptor to inhibitory synapses freezes excitatory synaptic strength and preserves memory. Neuron, 109(1), 123-134.e4.

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Electrophysiology and Anatomy of Cre-Mouse Lines

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All procedures were approved by the Animal Care Committee of the Montreal Neurological Institute at McGill University in accordance with Canadian Council on Animal Care guidelines (protocol MNI-7839). The subjects (n = 43 for electrophysiology and n = 6 for anatomy) were adult (>8 weeks old) male mice bred by crossing wild-type females on C57BL/6J background (Jackson Laboratories, 000664) with either homozygous male VGAT-IRES-Cre mice (Jackson Laboratories, 028862; n = 38), PV-IRES-Cre mice (Jackson Laboratories, 017320; n = 2) or SST-IRES-Cre mice (Jackson Laboratories, 013044; n = 2). One additional mouse implanted with a Neuropixel probe (Fig. 1a–c) was a cross-bred C57BL/6J and FVB (Jackson Laboratory, 001800). Mice were kept in standard conditions (room temperature and 50% humidity) on a 12-h light/12-h dark cycle and were housed in group cages (2–5 mice per cage) before electrode implantation surgery and individually afterwards.

Duszkiewicz A.J., Orhan P., Skromne Carrasco S., Brown E.H., Owczarek E., Vite G.R., Wood E.R, & Peyrache A. (2024). Local origin of excitatory–inhibitory tuning equivalence in a cortical network. Nature Neuroscience, 27(4), 782-792.

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Transgenic Mouse Breeding Protocol

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A total of 138 mice were used in these experiments. All experiments were approved by the Pennsylvania State University Institutional Animal Care and Use Committee. Adult (over 8 weeks of age) male and female C57BL/6 J (stock #000664, The Jackson Laboratory), hemizygous SST-IRES-Cre mice (stock #013044, The Jackson Laboratory) and Ai9 reporter mice (stock #007909, The Jackson Laboratory) on C57BL6/J background were bred in-house and genotyped by standard PCR protocol (additional detail available in supplemental methods).

Dao N.C., Brockway D.F., Suresh Nair M., Sicher A.R, & Crowley N.A. (2021). Somatostatin neurons control an alcohol binge drinking prelimbic microcircuit in mice. Neuropsychopharmacology, 46(11), 1906-1917.

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Transgenic Mouse Model Generation

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Mice were bred and housed in a controlled environment on a standard 12-hour light cycle (on at 6:00 am). Transgenic mice expressing tdTomato fluorescent protein in PFC interneurons were generated by crossing female PV-Cre mice (Jackson Laboratories, Stock No: 017320) or SST-IRES-Cre mice (Jackson Laboratories, Stock No: 028864) with male Rosa26-loxP-STOP-loxP-CAG-tdTomato "Ai9" mice (Jackson Laboratories, Stock No: 007909). All breeding strains were congenic on a C57BL/6J genetic background. Only female PV-Cre mice were used for breeding to mitigate PV-Cre driven recombination that can occur in sperm. All breeding mice were homozygous for the respective transgene, generating heterozygous PV-tdTomato and SST-tdTomato mice suitable for experimentation. Mice were defined as female or male by their external genitalia and the current studies are limited by this definition.

Joffe M.E., Winder D.G., & Conn P.J. (2020). Contrasting adaptations to synaptic physiology of prefrontal cortex interneuron subtypes in a mouse model of binge drinking.

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