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4 protocols using rimonabant hydrochloride

1

Optimized Reagent Procurement for Research

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Dopamine hydrochloride (#H-8502), S-(-)3-PPP (#P-103), Terguride (#T-134), angiotensin II (#A9525), and buprenorphine hydrochloride (#B9275) were purchased from Sigma-Aldrich; sulpiride (#0895), quinpirole (#1061), bromocriptine (#0427), olmesartan (#4616), Dynorphin B (#3196), naloxone hydrochloride (#0599), WN552122 (#1038), and rimonabant hydrochloride (#0923) from Tocris; DAMGO (#024-10) from Phoenix Pharmaceuticals, and rapamycin (#tlrl-rap) from Invivogen and morphine sulfate (#M1167) from Spectrum Chemicals.
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2

Preparing Compound Stock Solutions

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ACEA, ghrelin (human), rimonabant hydrochloride, and YIL 781 hydrochloride were purchased from Tocris Bioscience (Bristol, United Kingdom). Concentrated (10 mM) stock solutions prepared in DMSO, milli-Q® H2O (Merck/Millipore, Darmstadt, Germany), or ethanol were stored at −20°C. In each experimental session, aliquots of concentrated solutions of compounds were thawed and conveniently diluted in the appropriate experimental solution.
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3

Quantifying Neurotransmitter Receptors

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Dopamine hydrochloride (#H-8502) and angiotensin II (#A9525) were purchased from Sigma-Aldrich (St. Louis, MO, USA); sulpiride (#0895), olmesartan (#4616), Dynorphin B (#3196), naloxone hydrochloride (#0599), WN552122 (#1038) and rimonabant hydrochloride (#0923) from Tocris (Bristol, UK); DAMGO (#024-10) from Phoenix Pharmaceuticals and rapamycin (#tlrl-rap) from Invivogen (San Diego, CA, USA).
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4

Cannabidiol Modulates Astrocytic Bioenergetics

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Primary astrocytes were isolated from C57BL mice and were then exposed to bacterial endotoxin lipopolysaccharide ((LPS; Escherichia coli O26:B6; No 297-473-0, Sigma-Aldrich, Darmstadt, Germany)) to induce in ammation. The ability of (CBD ≥ 99% (HPLC); Tocris Bioscience, UK) to regulate the astrocytic mitochondrial bioenergetic pro le, inhibit the release of pro-in ammatory cytokines and modulate intracellular and mitochondrial Reactive Oxygen Species (ROS) production was evaluated in LPSstimulated astrocytes. CB1 receptor antagonist (SR141716A or Rimonabant hydrochloride, no 0923, Tocris Bioscience, UK)) was used to evaluate whether binding of CBD to CB1 receptor is essential for the restoration of LPS-induced ROS production, metabolic aberration, and in ammation in primary astrocytes.
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