4-µm wax sections were deparaffinized, hydrated, and preincubated with 10% goat serum in PBS for 1 h at RT. Sections were incubated overnight at 4°C with primary antibodies (FGF-2, Abcam; ab8880 at 1:100 dilution; perlecan, Invitrogen; MA5-14641 at 1:100 dilution; and aggrecan, Abcam; ab3778 at 1:150) in 10% goat serum followed by a wash and incubation with their corresponding secondary antibodies conjugated with Alexa Fluor 488, 594, and 647 (Life Technologies; 1;100 dilution) for 1 h at RT. Images were captured with a Zeiss LSM700 confocal microscope using Zen blue software. A 20×/0.8-NA objective was used, with air medium at RT.
Perlecan
Manufactured by Thermo Fisher Scientific
Perlecan is a lab equipment product designed for use in various research and scientific applications. It functions as a versatile and durable component for laboratory equipment and instrumentation. The core purpose of Perlecan is to provide reliable performance and support for the efficient operation of laboratory workflows.
Automatically generated - may contain errors
Lab products found in correlation
Fibroblast growth factor 2 (fgf2), by Abcam (1 mentions)
Aggrecan, by Abcam (1 mentions)
Lsm 700 confocal microscope, by Zeiss (1 mentions)
Ab3778, by Abcam (1 mentions)
Axiovert 40 cfl inverted microscope, by Zeiss (1 mentions)
Laminin, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488 conjugated anti rat, by Thermo Fisher Scientific (1 mentions)
2 protocols using perlecan
1
Immunofluorescent Localization of ECM Proteins
2
Immunofluorescence Analysis of C-dECM Components
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The C-dECMs and expanded human cells (to prepare C-dECMs) treated with 1% bovine serum albumin (BSA) (cat. no. BP1600, Fisher Scientific) for 1 h were incubated with primary antibodies against fibronectin [Clone 7.1; cat. no. HFN-3, Developmental Studies Hybridoma Bank (DSHB), Iowa City, IA], type IV collagen (cat. no. M3F7, DSHB), laminin (cat. no. Pa5-16287, Invitrogen, Waltham, MA), and perlecan (cat. no. sc-33707, Santa Cruz Biotechnology, Inc., Dallas, TX). Alexa Fluor Plus 555-conjugated anti-mouse secondary antibody (cat. no. A-32773, Invitrogen) was used to detect fibronectin and type IV collagen. Alexa Fluor 488-conjugated anti-rabbit (cat. no. A-11008, Invitrogen) and Alexa Fluor 488-conjugated anti-Rat (cat. no. A-11006, Invitrogen) were used as secondary antibodies to detect laminin and perlecan, respectively. Fluorescent intensity was visualized under a Zeiss Axiovert 40 CFL Inverted Microscope (Zeiss Oberkochen, Germany) using a 20 × objective lens.
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