Au480 clinical chemistry analyzer

Manufactured by Beckman Coulter

Sourced in United States

The AU480 Clinical Chemistry Analyzer is a fully automated, high-throughput instrument designed for clinical chemistry testing. It utilizes photometric and potentiometric detection methods to analyze a wide range of clinical chemistry parameters from various sample types. The AU480 is capable of performing routine and specialized clinical chemistry tests to support the diagnostic needs of medical laboratories.

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Spelling variants of this product

AU480 (221 citations) AU480 Chemistry Analyzer (113 citations) AU480 analyzer (29 citations) AU480 clinical analyzer (3 citations) Clinical Chemistry analyzer (3 citations)

20 protocols using au480 clinical chemistry analyzer

NEFA Measurement from Frozen Plasma

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Frozen EDTA-plasma aliquots were thawed in a fridge at 2–4 °C for 1–2 h. Subsequently, samples were shortly vortexed, centrifuged (5,000× g, 10 min, 8 °C, Biofuge Fresco, Heraeus) and analyzed within one hour. NEFA measurements were performed using an AU480 clinical chemistry analyzer (Beckman–Coulter) with the NEFA HR reagent kit (Wako Diagnostics) with corresponding calibrator and controls.

Backman M., Flenkenthaler F., Blutke A., Dahlhoff M., Ländström E., Renner S., Philippou-Massier J., Krebs S., Rathkolb B., Prehn C., Grzybek M., Coskun Ü., Rothe M., Adamski J., de Angelis M.H., Wanke R., Fröhlich T., Arnold G.J., Blum H, & Wolf E. (2019). Multi-omics insights into functional alterations of the liver in insulin-deficient diabetes mellitus. Molecular Metabolism, 26, 30-44.

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Neonatal Piglet Metabolic Profiling

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For clinical-chemical analysis, frozen plasma samples derived from non-fasted 3-day-old piglets were thawed for 1 h at room temperature, mixed thoroughly and then centrifuged (10 min, 5000×g at 8 °C) and afterwards analyzed immediately using an AU480 clinical chemistry analyzer (Beckman Coulter) and adapted reagent kits from Beckman Coulter, Randox (Glycerol) or FUJIFILM Wako Chemicals Gmbh (NEFA) as described previously [38 ]. Insulin concentration was determined with ultrasensitive insulin ELISA from EDTA plasma (#10-1132-01, Mercodia) collected from newborn piglets before first milk intake. The homeostatic model assessment for insulin resistance index (HOMA-IR) [39 (link)] for estimating insulin resistance at fasting conditions was calculated using the formula: HOMA-IR = fasting plasma insulin (μU/mL) × fasting plasma glucose (mg/dL)/405. The ‘QUantitative Insulin sensitivity ChecK’ (QUICKI) index [40 (link)] was calculated with the formula: QUICKI = 1/[log(insulin (mU/L)) + log(glucose (mg/dL))].

Shashikadze B., Valla L., Lombardo S.D., Prehn C., Haid M., Riols F., Stöckl J.B., Elkhateib R., Renner S., Rathkolb B., Menche J., Hrabĕ de Angelis M., Wolf E., Kemter E, & Fröhlich T. (2023). Maternal hyperglycemia induces alterations in hepatic amino acid, glucose and lipid metabolism of neonatal offspring: Multi-omics insights from a diabetic pig model. Molecular Metabolism, 75, 101768.

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Analyzing Serum Biomarkers in Anesthetized Barramundi

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Blood collection protocols from three fish/tank were described in our earlier study (1 (link)). Following blood collection using from anesthetized barramundi (8 mg/L of AQUI-S®) by puncturing the caudal vein, blood was allowed to clot for 4 h on ice and then centrifuged to separate the serum. The collected sera were preserved at −80°C for further analysis. Creatine kinase (CK; OSR6179) calcium (OSR60117), Mg (OSR6189), inorganic phos (OSR6122), iron (OSR6186), gamma-glutamyltransferase (GGT; OSR6020), and albumin (catalog code OSR6102) were run on a AU480 Clinical Chemistry Analyzer (Beckman Coulter Australia Pty Ltd., Lane Cove West, NSW) using Beckman Coulter clinical chemistry kits. A phase haptoglobin assay kit was used to analyze serum haptoglobin following the manufacturer’s instructions (Tridelta Development Ltd., Co. Wicklow, Ireland).

Chaklader M.R., Howieson J., Foysal M.J., Hanif M.A., Abdel-Latif H.M, & Fotedar R. (2023). Fish waste to sustainable additives: Fish protein hydrolysates alleviate intestinal dysbiosis and muscle atrophy induced by poultry by-product meal in Lates calcarifer juvenile. Frontiers in Nutrition, 10, 1145068.

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Liver and Metabolic Biomarkers Assay

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Plasma albumin, bilirubin, blood urea nitrogen, creatinine, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were measured using an AU480 Clinical Chemistry Analyzer (Beckman Coulter, Providence, RI). Basal blood glucose and plasma insulin were assessed using an Accu‐Chek glucometer (Roche Diagnostics, Basel, Switzerland) and the Ultrasensitive Mouse Insulin Enzyme‐Linked Immunosorbent Assay kit (Crystal Chem, Downers Grove, IL), respectively. Plasma levels of TAG and cholesterol were measured using the Infinity TAG and the Infinity Cholesterol kits, respectively (Thermo Fisher Scientific, Waltham, MA), with Multiconstituent Calibrator 1E65‐04 (Abbott, North Chicago, IL) used as reference. For hepatic TAG measurements, lipids were extracted from liver samples and analyzed using straight‐phase high‐performance liquid chromatography as described.21 Hepatic collagen deposition was assessed in liver homogenates using the Hydroxyproline Colorimetric Assay kit (Sigma‐Aldrich, St. Louis, MO).

Nuñez‐Durán E., Aghajan M., Amrutkar M., Sütt S., Cansby E., Booten S.L., Watt A., Ståhlman M., Stefan N., Häring H., Staiger H., Borén J., Marschall H, & Mahlapuu M. (2017). Serine/threonine protein kinase 25 antisense oligonucleotide treatment reverses glucose intolerance, insulin resistance, and nonalcoholic fatty liver disease in mice. Hepatology Communications, 2(1), 69-83.

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Plasma Chemistry Analysis via Cardiac Puncture

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Blood samples were collected by cardiac puncture at time of sacrifice. Plasma chemistry values were measured on the AU480 Clinical Chemistry Analyzer (Beckman Coulter).

Huang L., Damle S.S., Booten S., Singh P., Sabripour M., Hsu J., Jo M., Katz M., Watt A., Hart C.E., Freier S.M., Monia B.P, & Guo S. (2015). Partial Hepatectomy Induced Long Noncoding RNA Inhibits Hepatocyte Proliferation during Liver Regeneration. PLoS ONE, 10(7), e0132798.

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Serum Lipid and Liver Enzyme Analysis

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Blood was collected at necropsy into Becton Dickinson Vacutainer serum separating tubes (ref # 367981) and serum prepared according to manufacturer’s directions. Samples of serum (10 μL) were analyzed for cholesterol (total cholesterol, triglycerides, LDL, and HDL), liver enzymes (ALP, ALT, and AST), and glucose using an AU480 clinical chemistry analyzer (Beckman Coulter Inc., Brea, CA, USA). Reagents and calibrators used to measure alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), glucose (Glu), triglyceride (Trig), high density lipoprotein (HDL), and cholesterol (Chol), were purchased from Beckman Coulter Inc. (Melville, NY, USA). Reagents used to measure low density lipoprotein (LDL) were purchased from Diazyme Laboratories (Poway, CA, USA). Lipid panel measurements were conducted on serum samples collected at PND 22, Week 6, and Week 18. Liver enzymes were measured for Week 18 samples only.

Cope H.A., Blake B.E., Love C., McCord J., Elmore S.A., Harvey J.B., Chappell V.A, & Fenton S.E. (2021). Latent, sex-specific metabolic health effects in CD-1 mouse offspring exposed to PFOA or HFPO-DA (GenX) during gestation. Emerging contaminants, 7, 219-235.

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Multiplex Plasma Biomarkers Analysis

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Plasma lipid and glucose levels were determined using an AU480 clinical chemistry analyzer (Beckman–Coulter) and adapted reagents from Beckman–Coulter (glucose, cholesterol, triglycerides and lactate) evtl Wako-Chemicals (NEFA) and Randox (Glycerol) as previously described (82 (link)). We use a multiplex assay platform to measure the concentration of leptin, FGF21 and insulin in plasma samples. It is an electroluminescence-linked immunosorbent assay based on the Mesoscale Discovery technology (U-Plex, Mesoscale Diagnostics, Rockville, MD, USA). A 10 spot MSD plate is coated with anti-insulin, anti-FGF21 and anti-leptin antibodies (previously treated with the corresponding spot-linkers). Plasma samples are diluted 1:2 and incubated for 1 h. After that, the samples are incubated for 1 h with Sulfotag conjugated detection antibodies (second antibodies) before they are analyzed in the MSD plate reader. Coated and detection antibodies for leptin, FGF21 and insulin are provided as U-Plex antibodies from MSD. MSD discovery workbench is used as analysis software.

Lucienne M., Gerlini R., Rathkolb B., Calzada-Wack J., Forny P., Wueest S., Kaech A., Traversi F., Forny M., Bürer C., Aguilar-Pimentel A., Irmler M., Beckers J., Sauer S., Kölker S., Dewulf J.P., Bommer G.T., Hoces D., Gailus-Durner V., Fuchs H., Rozman J., Froese D.S., Baumgartner M.R, & de Angelis M.H. (2023). Insights into energy balance dysregulation from a mouse model of methylmalonic aciduria. Human Molecular Genetics, 32(17), 2717-2734.

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Plasma Biomarker Quantification Protocol

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Plasma was collected from every subject as previously described (Barbayianni, Ninou, et al. 2018). In brief, after euthanasia of the animals, the blood was collected through the inferior vena cava, and EDTA was added to a final concentration of 10%. The samples were then centrifuged for 20 min at 2000 g at 4 °C. Plasma was transferred and stored at 4 °C until biochemical analysis was performed using a Beckman Coulter AU480 Clinical Chemistry Analyzer based at the BSRC ‘Alexander Fleming’ phenotyping facility for the estimation of Alanine Transaminase (ALT) (OSR6107, Beckman Coulter, Brea, CA, USA) and Aspartate Transaminase (AST) (OSR6109, Beckman Coulter, Brea, CA, USA) levels.

Galaris A., Fanidis D., Stylianaki E.A., Harokopos V., Kalantzi A.S., Moulos P., Dimas A.S., Hatzis P, & Aidinis V. (2022). Obesity Reshapes the Microbial Population Structure along the Gut-Liver-Lung Axis in Mice. Biomedicines, 10(2), 494.

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Plasma and Urine Electrolyte Analysis

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Plasma and urine electrolytes (K⁺, Na⁺, Cl⁻) were measured at the Animal Phenotyping Facility at Max-Delbrück-Centrum (MDC) Berlin using an AU480 Clinical Chemistry Analyzer (Beckman Coulter).

Schewe J., Seidel E., Forslund S., Marko L., Peters J., Muller D.N., Fahlke C., Stölting G, & Scholl U. (2019). Elevated aldosterone and blood pressure in a mouse model of familial hyperaldosteronism with ClC-2 mutation. Nature Communications, 10, 5155.

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Plasma Chemistry Analysis via Cardiac Puncture

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Blood samples were collected by cardiac puncture at time of sacrifice. Plasma chemistry values were measured on the AU480 Clinical Chemistry Analyzer (Beckman Coulter).

Huang L., Low A., Damle S.S., Keenan M.M., Kuntz S., Murray S.F., Monia B.P, & Guo S. (2018). Antisense suppression of the nonsense mediated decay factor Upf3b as a potential treatment for diseases caused by nonsense mutations. Genome Biology, 19, 4.

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