Rea isotype control

Manufactured by Miltenyi Biotec

The REA Isotype control is a laboratory reagent used in flow cytometry experiments. It serves as a control to help identify non-specific binding of antibodies to cells. The REA Isotype control is designed to match the isotype of the primary antibody being used in the experiment, ensuring a proper comparison and interpretation of the specific antibody staining.

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Lab products found in correlation

Cd44v6, by Thermo Fisher Scientific (2 mentions) Tspan8, by Miltenyi Biotec (2 mentions) Iggk2b, by BioLegend (2 mentions) Cd104, by Miltenyi Biotec (2 mentions) Iggk1, by Miltenyi Biotec (2 mentions) C met, by Miltenyi Biotec (2 mentions) Cd44v6, by Miltenyi Biotec (2 mentions) Cd104, by Thermo Fisher Scientific (2 mentions) C met, by Thermo Fisher Scientific (2 mentions) Epcam, by Miltenyi Biotec (2 mentions) Epcam, by BioLegend (2 mentions)

2 protocols using rea isotype control

Multimarker Flow Cytometry Profiling

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The following antibodies were used, adding 1 µL of each antibody to our sample per our previously published work^{6 (link)}: EpCAM (Biolegend), CD104 (Thermo Fisher), c-Met (Thermo Fisher), CD44v6 (Thermo Fisher), Tspan8 (Miltenyi). The isotype controls used were the Biolegend IgGk2b (to match EpCAM, CD104) and IgGK1 (c-Met, CD44v6) alongside the Miltenyi REA Isotype control (to match Tspan8). Samples were run at a flow rate of ɸ = 2.5 mL/h.

Lin A.A., Shen H., Spychalski G., Carpenter E.L, & Issadore D. (2023). Modeling and optimization of parallelized immunomagnetic nanopore sorting for surface marker specific isolation of extracellular vesicles from complex media. Scientific Reports, 13, 13292.

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Characterization of Circulating Tumor Cells

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the following antibodies were used, adding 1 μL of each antibody to our sample per our previously published work [6 ]: EpCAM (Biolegend), CD104 (Thermo Fisher), c-Met (Thermo Fisher), CD44v6 (Thermo Fisher), Tspan8 (Miltenyi). The isotype controls used were the Biolegend IgGk2b (to match EpCAM, CD104) and IgGK1 (c-Met, CD44v6) alongside the Miltenyi REA Isotype control (to match Tspan8). Samples were run at a flow rate of = 2.5 mL/hr.

Lin A.A., Shen H., Spychalski G., Carpenter E.L, & Issadore D. (2023). Parallelized immunomagnetic nanopore sorting: modeling, scaling, and optimization of surface marker specific isolation of extracellular vesicles from complex media. Research Square.

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