Dp71 color camera

Manufactured by Olympus

Sourced in Japan

The DP71 is a color camera from Olympus designed for use in microscopy applications. The camera features a high-resolution CCD sensor and delivers accurate color reproduction. It is capable of capturing detailed images and facilitating scientific observation and analysis.

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Lab products found in correlation

Eclipse 90i, by Nikon (1 mentions) Bx61 microscope, by Olympus (1 mentions) Optimal cutting temperature compound, by Thermo Fisher Scientific (1 mentions) Eclipse microscope, by Olympus (1 mentions)

Spelling variants of this product

DP71 camera (288 citations) DP71 High Resolution Color Digital Camera (2 citations) DP71 color digital camera (2 citations)

3 protocols using dp71 color camera

Morphological Impacts of ZnO Nanoparticles on Zebrafish Embryos

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Morphological characteristics were examined at 96 hpf for embryos exposed to the highest concentration of ZnO NPs (100 µg/mL). The characteristics analyzed were: (1) body length (µm); (2) eye size (area normalized to body length); (3) morphology of the lower jaw (presence of protruding mouth); (4) pigmentation pattern (higher or lower pigmentation compared to controls). After euthanasia, embryos were transferred to 4% paraformaldehyde in 0.1 M phosphate buffer for at least 24 h. Embryos were then mounted in the lateral view in 1% low melting point agarose and images were taken using a 4X Plan Apo lens (0.2 NA; Nikon, Tokyo, Japan) of a bright field microscope (Eclipse 90i; Nikon) equipped with an Olympus DP71 color camera. Body length was considered as the distance from the mouth to the start of the caudal fin. The area of the eye was measured using Fiji [82 (link)], selecting a region of interest (ROI), and was normalized to body size (area to body length ratio).

Valdiglesias V., Alba-González A., Fernández-Bertólez N., Touzani A., Ramos-Pan L., Reis A.T., Moreda-Piñeiro J., Yáñez J., Laffon B, & Folgueira M. (2023). Effects of Zinc Oxide Nanoparticle Exposure on Human Glial Cells and Zebrafish Embryos. International Journal of Molecular Sciences, 24(15), 12297.

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Histological Characterization of Tissue Samples

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For histological characterization, one-third of each tissue was embedded in optimal cutting temperature compound (Thermo Fisher Scientific, Waltham, MA) and sectioned at 8 μm. Sections were stained with hematoxylin and eosin (H&E) to characterize the groups of study. Images were captured with an Olympus BX61 microscope connected to a DP71 color camera (Olympus, Tokyo, Japan).

Baldan-Martin M., Martin-Rojas T., Corbacho-Alonso N., Lopez J.A., Sastre-Oliva T., Gil-Dones F., Vazquez J., Arevalo J.M., Mourino-Alvarez L, & Barderas M.G. (2020). Comprehensive Proteomic Profiling of Pressure Ulcers in Patients with Spinal Cord Injury Identifies a Specific Protein Pattern of Pathology. Advances in Wound Care, 9(5), 277-294.

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Chikungunya Virus RNA Detection in Mouse Spleen

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Four-week-old vehicle or AV-treated WT mice were inoculated subcutaneously in the foot with 10³ FFU of CHIKV LR 2006. At 3 dpi, animals were euthanized and perfused extensively with PBS. Spleens were harvested and fixed in 4% paraformaldehyde (PFA) for 24 h. Tissues were embedded in paraffin, and sections were stained with hematoxylin and eosin. RNA in situ hybridization was performed using the RNAscope 2.5 HD Assay (Brown Kit) according to the manufacturer’s instructions (Advanced Cell Diagnostics). Briefly, sections were deparaffinized, treated with H₂O₂ and Protease Plus prior to probe hybridization. A probe specifically targeting the CHIKV RNA (strain La Reunion) (Advanced Cell Diagnostics, 481891) was custom-designed and used for in situ hybridization experiments. Tissues were counterstained with Gill’s hematoxylin. An uninfected mouse was used as a negative control and stained in parallel. Tissue sections were visualized using a Nikon Eclipse microscope equipped with an Olympus DP71 color camera.

Winkler E.S., Shrihari S., Hykes BL J.r., Handley S.A., Andhey P.S., Huang Y.J., Swain A., Droit L., Chebrolu K.K., Mack M., Vanlandingham D.L., Thackray L.B., Cella M., Colonna M., Artyomov M.N., Stappenbeck T.S, & Diamond M.S. (2020). The intestinal microbiome restricts alphavirus infection and dissemination through a bile acid-type I IFN signaling axis. Cell, 182(4), 901-918.e18.

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